COLLECTION OF SAMPLES

     

PERIPHERAL BLOOD:

Most commonly used for diagnosis of different chromosome disorders. (Collection should be done aseptically in sodium heparin vaccutainer).

DNA LABS INDIA is the first laboratory in India to use the latest PCR method with a mass spectrometer. This is the one of the most reliable technology available. This technique contrasts to the older technique of singleplexing PCR. It takes a significant investment of time and money to use the newer method. Therefore, the older technique is what is used by most laboratories today. Another important thing is that our entire process is completely automated.

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PRODUCTS OF CONCEPTION:

Important in case of abortions. Should be collected in sterile container having tissue culture media/normal saline with few drops of antibiotics. (Few bits of placental tissue are required. Tissue should be collected aseptically using ovum forceps. Cervical portion should be cleaned by normal saline. Suction material is always contaminated and cannot be used for genetic studies).

AMNIOTIC FLUID:

Collected between 15th and 17th week of pregnancy (always collected in sterile container recommended for tissue culture).

CHORIONIC VILLUS SAMPLING:

Collected between 8th and 10th week of pregnancy (always collected in tissue culture media containing few drops of antibiotics)

BONE MARROW CULTURES:

Performed in blood disorders (collect in sodium heparin vaccutainer containing transport media (if available) / sodium heparin vaccutainer. Sample should be transported immediately to obtain quality metaphases. Outstation samples should reach the lab within 24 hours of collection of bone marrow samples (NB: in cases of leukemia peripheral blood should only be sent in case of unavailability of bone marrow due to the failure rate of around 40%).

FISH (FLUORESCENCE IN SITU HYBRIDIZATION):

This is routinely performed on prenatal samples for immediate reporting for common aneuploidies (13, 18, 21, X & Y). FISH for leukemia’s are performed on peripheral blood/bone marrow samples collected in sodium heparin vaccutainer (BCR/ABL, PML/RARA, etc).

DNA STUDIES:

  • For all the DNA studies, the blood sample should be collected in EDTA vaccutainer.
  • The tissue samples should be collected in sterile container having normal saline.
  • Vaginal swabs should be transported directly in sterile packs for HPV (human Papilloma virus).
  • All the samples should be transported at ambient temperature.