Genetic AML1/ETO FISH DNA Test
A positive result (AML1-ETO fusion) is reported when the percent of cells with an abnormality exceeds the normal reference range for the probes. The detection of an abnormal clone indicates a diagnosis of AML with the 8;21 translocation. A negative result indicates no 8;21translocation was observed but does not rule out the presence of a neopl
A dual-color, dual-fusion FISH analysis performed on interphase cells using a probe for the AML1 (RUNX1) gene on chromosome 21q22 and a probe for the ETO (RUNX1T1) gene on chromosome 8q22; analysis of 200 interphase cells for diagnostic specimen; analysis of 500 interphase cells for minimal residual disease assessment
Fluorescence in-situ Hybridization (FISH) is a branch of cancer genetics that focuses on detecting and locating the presence or absence of specific DNA sequences on chromosomes. FISH is also referred to as Molecular Cytogenetics.
To perform FISH, cells are fixed onto the surface of a slide, and then the slide is treated so that the chromosomal DNA is denatured into single strands.
Then, special fluorescently labeled DNA probes are applied onto the slide. The DNA probes are small pieces of single stranded DNA with a sequence from the gene of interest. Probes are only able to hybridize with their complimentary sequence, which is the gene or locus of interest on a particular chromosome. The DNA probes are allowed to hybridize with the denatured chromosomal DNA, and any excess probes are washed away.
After the excess probes are washed away, the slide is then viewed under a fluorescence microscope. The fluorescently labeled molecules reveal the physical location of the gene or locus of interest. Pathologists will then use specified cut off values to assess the results of the FISH test.
Fluorescence In Situ Hybridization (FISH)
Fluorescence in situ hybridization (FISH) is a molecular diagnostic technique utilizing labeled DNA probes to detect or confirm gene or chromosome abnormalities. It is often used in cancer diagnosis. The sample DNA (metaphase chromosomes or interphase nuclei) is first denatured, a fluorescently labeled probe of interest is then added to the denatured sample mixture and hybridizes with the sample DNA at the target site as it re-anneals back into a double stranded DNA. The probe signal can then be seen through a fluorescent microscope and the sample DNA can be scored for the presence or absence of the signal. Unlike most other techniques used to study chromosomes, FISH does not have to be performed on cells that are actively dividing. This makes it a very versatile procedure. Uses encompass a wide range of applications such as the detection of aneuploidy, constitutional microdeletion syndromes as well as rearrangements. These aberrations have clinical implications for numerous genetic diseases such as leukemia, lymphoma, solid tumors, autism and other developmental syndromes. FISH probes are commonly made from BAC clones.
Leukemias, lymphomas, other hematopoietic malignancies and some types of solid tumors can often be characterized by specific chromosomal and genetic abnormalities. Interphase FISH does not require cell division. FISH can be used to identify those specific abnormalities that are more common. Chromosome analysis may be ordered in conjunction with or prior to FISH testing.
FISH studies are used to determine the presence of a known or suspected abnormality. This is particularly useful when there are few or no dividing cells in the sample for cytogenetic analysis.
- When requested, fluorescence in situ hybridization (FISH) can be performed as an adjunct to a Integrated Oncology cytogenetics study
- Interpretation and report (added to all Interphase or Metaphase FISH)
- New probes/FISH tests are frequently added to this list. Please contact Client Services if the test you desire is not included in the list. For additional probes, refer to our FISH Probe Library.
|Reporting Time :||3 Days (From the day sample reaches our Lab)|
|Whole blood:||Send in Sodium Heparin Vacutainer (2ml)|
|Bone Marrow / Peripheral blood||Bone Marrow / Peripheral blood|
At DNA Labs India, we are dedicated to quality DNA testing. We know that you need accurate, reliable results for such an important test, and we are committed to providing you with high quality DNA testing services. By choosing DNA Labs India, you will receive the highest quality, most conclusive results in the industry.
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