Genetic CHROMOSOMES 13, 18, 21, X & Y DNA Test


Genetic CHROMOSOMES 13, 18, 21, X & Y DNA Test

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The nucleus from each blastomere was fixed separately and fluorescence in-situ hybridization (FISH) using seven probes (13, 16, 18, 21, 22, X and Y) was performed.

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The pre-embryos were classified as uniformly normal. Abnormality rate was significantly increased in the pre-embryos with unevenly sized blastomeres and with increasing degree of fragmentation after fertilization. Applying criteria for good embryo quality significantly increased the rate of chromosomally normal pre-embryos.
The data demonstrate the high degree of genetic heterogeneity in a randomly selected pool of donated pre-embryos from an IVF programme. Further, we found that uniformity of blastomere size, degree of fragmentation and cleavage kinetics reflect the cytogenetic status of the pre-embryo and are therefore important in the selection of pre-embryos.