Genetic Inv(16) (AML M4Eo) DNA Test


Genetic Inv(16) (AML M4Eo) DNA Test

19867 reviews
5.00 out of 5

Cost 6,000/- Rs

Extensive immunologic marker analysis was performed to characterize the various leukemic cell populations in eight patients with inv(16) in association with acute myeloid leukemia with abnormal bone marrow eosinophilia (AML-M4Eo).

Call: (079) 30925079
Virtually all AML-M4Eo cells were positive for the pan-myeloid marker . In addition, the AML were partly positive for terminal deoxynucleotidyl transferase (TdT), and HLA-DR. Double immunofluorescence stainings demonstrated coexpression of the CD2 antigen and myeloid markers and allowed the recognition of multiple AML subpopulations. The CD2 antigen was expressed by immature AML cells and more mature monocytic AML cells , whereas TdT expression was exclusively found in the cell population. The eight AML-M4Eo cases not only expressed the CD2 antigen, but also its ligand . Culturing of AML-M4Eo cell samples showed a high spontaneous proliferation in all three patients tested. Addition of a mixture of CD2 antibodies against the epitopes diminished cell proliferation in two patients with high CD2 expression, but no inhibitory effects were found in the third patient with low frequency and low density of CD2 expression. These results suggest that high expression of the CD2 molecule in AML-M4Eo stimulates proliferation of the leukemic cells, which might explain the high white blood cell count often found in this type of AML.