12S rRNA Sequencing Test
Also known as: 12S rRNA gene sequencing, Mitochondrial DNA sequencing
12S rRNA Sequencing Test test available at DNA Labs India for ₹5,600. Uses PCR, Sequencing on Extracted DNA samples. Results in 2 weeks. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The purpose of 12S rRNA sequencing is to detect mutations in the 12S ribosomal RNA gene of the mitochondrial genome, which can help diagnose mitochondrial disorders. These disorders often present with diverse symptoms, and this test provides a targeted approach to identify genetic causes, aiding in clinical management and family counseling.
- Test Code
- 3389
- Price
- ₹5,600
- Sample Type
- Extracted DNA
- Result Time
- 2 weeks
- Fasting Required
- No
- Method
- PCR, Sequencing
Sample Collection
No special preparation is required. Ensure the sample is collected as per standard protocols.
Method: DNA extraction from blood or tissue sample
Laboratory Analysis
A trained professional will collect a blood or tissue sample for DNA extraction.
Report Delivery
The sample will be processed in the laboratory for DNA extraction and sequencing.
Timeline: 2 weeks
Patient Instructions
About This Test
Who Should Get This Test
The purpose of 12S rRNA sequencing is to detect mutations in the 12S ribosomal RNA gene of the mitochondrial genome, which can help diagnose mitochondrial disorders. These disorders often present with diverse symptoms, and this test provides a targeted approach to identify genetic causes, aiding in clinical management and family counseling.
How to Prepare
- Provide a blood sample or tissue as directed by the healthcare provider
- Ensure proper labeling and handling of the sample
- Avoid contamination during collection
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"This test is crucial for patients with suspected mitochondrial disorders, aiding in accurate diagnosis and management, especially in cases with family history or unexplained symptoms."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed or contaminated sample
- Insufficient sample volume
- Improperly labeled samples
Understanding Your Results
Consult a doctor if you experience symptoms of mitochondrial disorders such as persistent fatigue, muscle weakness, or neurological issues, or if the test results are abnormal.
Limitations
- ⚠May not detect all mitochondrial mutations
- ⚠Requires confirmation with additional tests for comprehensive diagnosis
- ⚠Limited to the 12S rRNA gene region
Risks & Considerations
- ●Minimal risks associated with sample collection, such as slight discomfort or bruising
- ●No significant health risks from the test itself
Interfering Factors
- ●Sample contamination
- ●Degraded DNA quality
- ●Technical errors during sequencing
Compare With Similar Tests
| Test | 12S rRNA Sequencing | Whole Mitochondrial Genome Sequencing | Next-Generation Sequencing (NGS) Panel | Lactate/Pyruvate Ratio Test |
|---|---|---|---|---|
| Comparison | 12S rRNA Sequencing |
Frequently Asked Questions
What is 12S rRNA sequencing?
How much does 12S rRNA sequencing cost in India?
What are the symptoms of mitochondrial disorders?
How is the test performed?
Is fasting required for this test?
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Is the test covered by insurance?
What sample is needed for the test?
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How accurate is 12S rRNA sequencing?
Can this test detect all mitochondrial disorders?
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