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2x150 Hiseq Sequencing- 3 GB Test

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2x150 Hiseq Sequencing- 3 GB Test

Short Name: 2x150 Hiseq Sequencing

Also known as: High-Throughput Sequencing, NGS 2x150, Whole Exome Sequencing (if applicable)

2x150 Hiseq Sequencing- 3 GB Test test available at DNA Labs India for ₹7,500. Uses Next-Generation Sequencing (NGS), Illumina HiSeq Platform on Extracted DNA samples. Results in Results are typically available within 4 weeks from the time the sample is received at the laboratory.. Free home collection in 300+ cities across India.

Next-Generation Sequencing🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

The purpose of the 2x150 Hiseq Sequencing test is to detect genetic variations that may be responsible for a patient's symptoms or disease. It helps in: 1) Diagnosing genetic disorders, 2) Identifying carrier status for recessive conditions, 3) Guiding treatment decisions (pharmacogenomics), 4) Assessing risk for hereditary cancers, 5) Providing information for family planning, 6) Understanding unexplained developmental delays or congenital anomalies.

Test Code
6442
CPT Code
81479
ICD Code
Z13.89
Price
₹7,500
Sample Type
Extracted DNA
Result Time
Results are typically available within 4 weeks from the time the sample is received at the laboratory.
Fasting Required
No
Method
Next-Generation Sequencing (NGS), Illumina HiSeq Platform
Step 1

Sample Collection

No special preparation is required. However, if providing a blood sample, it is advisable to stay well-hydrated. Inform your doctor about any medications you are taking.

Method: Blood or saliva sample (DNA extraction required)

Step 2

Laboratory Analysis

A blood sample will be drawn from a vein in your arm, or a saliva sample may be collected. The procedure is quick and minimally invasive.

Step 3

Report Delivery

You can resume normal activities immediately. There are no restrictions after sample collection.

Timeline: Results are typically available within 4 weeks from the time the sample is received at the laboratory.

Patient Instructions

1
Before the Test:No special preparation is needed. However, it is important to provide a detailed clinical history and any prior genetic testing results to aid in interpretation.
2
During the Test:The test involves sequencing of your DNA sample. You do not need to do anything during the test.
3
After the Test:After the test, you will receive a comprehensive report. It is recommended to discuss the results with a genetic counselor or your physician.

About This Test

Who Should Get This Test

The purpose of the 2x150 Hiseq Sequencing test is to detect genetic variations that may be responsible for a patient's symptoms or disease. It helps in: 1) Diagnosing genetic disorders, 2) Identifying carrier status for recessive conditions, 3) Guiding treatment decisions (pharmacogenomics), 4) Assessing risk for hereditary cancers, 5) Providing information for family planning, 6) Understanding unexplained developmental delays or congenital anomalies.

How to Prepare

  • For blood sample: Use EDTA tube, mix gently.
  • For saliva sample: Use provided collection kit, avoid eating/drinking 30 minutes prior.
  • Label the sample with patient ID and date.
  • Store at room temperature if shipping within 24 hours, otherwise refrigerate.

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"This sequencing test provides a comprehensive view of the exome, aiding in the diagnosis of rare genetic disorders. It is particularly useful when clinical presentation is non-specific."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeExtracted DNA
Sample Volume1-2 µg
ContainerEppendorf tube
Collection MethodBlood or saliva sample (DNA extraction required)

Sample Stability

Blood: 7 days at 2-8°C
Extracted DNA: 1 year at -20°C
Saliva: 7 days at room temperature
Sample Rejection Criteria:
  • Hemolyzed blood sample
  • Clotted blood sample
  • Insufficient sample volume
  • Sample not labeled correctly
  • Sample received after prolonged storage

Understanding Your Results

The results of the 2x150 Hiseq Sequencing test are interpreted by clinical geneticists. Variants are classified according to ACMG guidelines as pathogenic, likely pathogenic, uncertain significance, likely benign, or benign. The report includes a list of clinically significant variants and their implications for the patient's health.
📊

Pathogenic

Clinical action: May explain the patient's condition; genetic counseling recommended.

📊

Likely Pathogenic

Clinical action: Likely disease-causing; further testing may be needed.

📊

Uncertain Significance

Clinical action: Insufficient evidence; additional family studies may help.

📊

Benign/Likely Benign

Clinical action: No clinical significance.

⚠️ When to Consult a Doctor:

If you have symptoms suggestive of a genetic disorder, or if you have a family history of a genetic condition, consult a geneticist or your primary care physician to discuss whether this test is appropriate for you.

Limitations

  • ⚠May not detect all types of mutations (e.g., large deletions, repeat expansions)
  • ⚠Variant interpretation may be limited by current knowledge
  • ⚠Not a whole-genome sequencing test; only targets exonic regions
  • ⚠Results may require confirmation by Sanger sequencing
  • ⚠Cannot detect all genetic causes of disease

Risks & Considerations

  • ●No physical risks associated with the test itself.
  • ●Possible psychological impact of learning genetic information.
  • ●Risk of incidental findings unrelated to the original reason for testing.

Interfering Factors

  • ●Poor quality or degraded DNA sample
  • ●Contamination with foreign DNA
  • ●Insufficient DNA quantity
  • ●Presence of PCR inhibitors
  • ●Low sequencing coverage in GC-rich regions

Compare With Similar Tests

Test2x150 Hiseq Sequencing- 3 GBWhole Exome Sequencing (WES)Whole Genome Sequencing (WGS)Targeted Gene Panel
Comparison2x150 Hiseq Sequencing- 3 GB

Frequently Asked Questions

What is 2x150 Hiseq Sequencing?
2x150 Hiseq Sequencing is a next-generation sequencing method that reads 150 base pairs from both ends of DNA fragments, producing 300 base pair reads. It is used for high-throughput genetic analysis.
What is the cost of the test?
The test costs INR 7500, which includes sequencing of 3GB data and a comprehensive report.
What is the turnaround time?
Results are typically available within 4 weeks after sample submission.
What sample is required?
Extracted DNA is required. We provide a free home sample collection service for blood or saliva samples.
Is fasting required?
No, fasting is not required for this test.
What kind of results will I get?
You will receive a report detailing any disease-causing mutations, carrier status, and potential health risks, along with recommendations for personalized treatment.
Can this test detect all genetic disorders?
No, it may not detect all types of mutations, such as large deletions or repeat expansions. It is best used for identifying single nucleotide variants and small indels in coding regions.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across many cities in India.
How should I prepare for the test?
No special preparation is needed. Just ensure you provide a good quality sample as per instructions.
Will my insurance cover this test?
Insurance coverage varies. Please check with your insurance provider. We also offer affordable self-pay options.
What is the difference between this and Whole Exome Sequencing?
This test may be a targeted or limited sequencing approach, while Whole Exome Sequencing covers all coding regions of the genome. The choice depends on clinical indication.
How accurate is this test?
The test is highly accurate for detecting variants in the targeted regions, with a mean depth of coverage ?30x. However, all clinically significant variants are confirmed by Sanger sequencing.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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