2x250 Miseq Sequencing-Flow Cell Test
Short Name: 2x250 MiSeq Flow Cell
Also known as: MiSeq 2x250 Sequencing, 2x250 Flow Cell Sequencing, NGS Flow Cell 2x250
2x250 Miseq Sequencing-Flow Cell Test test available at DNA Labs India for ₹250,000. Uses Next-Generation Sequencing (NGS) on Illumina MiSeq platform, Paired-end 2x250 bp reads on Extracted DNA samples. Results in Reports are delivered within 2 weeks from the date of sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The primary purpose of this test is to provide high-throughput DNA sequencing for clinical and research applications. It helps in identifying disease-causing mutations, guiding targeted therapy, and understanding genetic predispositions. It is also used for pathogen identification and antimicrobial resistance profiling.
- Test Code
- 6440
- CPT Code
- 81479
- ICD Code
- Z01.89
- Price
- ₹250,000
- Sample Type
- Extracted DNA
- Result Time
- Reports are delivered within 2 weeks from the date of sample receipt.
- Fasting Required
- No
- Method
- Next-Generation Sequencing (NGS) on Illumina MiSeq platform, Paired-end 2x250 bp reads
Sample Collection
No special preparation required. However, if blood sample is to be collected, inform the lab about any anticoagulant therapy.
Method: Blood or tissue sample for DNA extraction (performed by lab or referring facility)
Laboratory Analysis
Blood sample collection is routine; no special precautions.
Report Delivery
No restrictions. The extracted DNA will be used for sequencing.
Timeline: Reports are delivered within 2 weeks from the date of sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of this test is to provide high-throughput DNA sequencing for clinical and research applications. It helps in identifying disease-causing mutations, guiding targeted therapy, and understanding genetic predispositions. It is also used for pathogen identification and antimicrobial resistance profiling.
How to Prepare
- Provide a blood sample (5-10 mL in EDTA tube) or tissue biopsy as per clinical requirement.
- DNA extraction will be performed at the laboratory; ensure sample is transported at 2-8°C if not processed immediately.
- For outstation samples, use dry ice shipment to maintain DNA integrity.
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"This high-throughput sequencing service is essential for identifying clinically significant genetic variants, enabling precise diagnosis and targeted therapy planning."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed blood sample
- Insufficient DNA quantity (<100 ng)
- DNA degradation (fragmentation)
- Improper labeling or missing requisition form
Understanding Your Results
Consult a genetic counselor or physician if you have a family history of genetic disorders, if you are considering carrier screening, or if you have been diagnosed with cancer and need targeted therapy options.
Limitations
- ⚠Not suitable for whole-genome sequencing due to limited output
- ⚠May not detect large structural variants or repeat expansions
- ⚠Requires high-quality DNA; poor quality may lead to failed runs
- ⚠Bioinformatics interpretation requires expert review
- ⚠Variant pathogenicity may be uncertain in some cases
Risks & Considerations
- ●No direct physical risks as the test is performed on extracted DNA.
- ●Blood collection may cause minor bruising or discomfort.
- ●Psychological impact of unexpected genetic findings.
Interfering Factors
- ●Low DNA concentration or degraded DNA
- ●Contamination with RNA or proteins
- ●PCR inhibitors in the sample
- ●Incorrect library preparation
- ●Insufficient sequencing depth for rare variants
Compare With Similar Tests
| Test | 2x250 Miseq Sequencing-Flow Cell | 2x150 MiSeq Sequencing | NextSeq 550 Sequencing | Sanger Sequencing |
|---|---|---|---|---|
| Comparison | 2x250 Miseq Sequencing-Flow Cell |
Frequently Asked Questions
What is the cost of the 2x250 MiSeq Sequencing Flow Cell?
What sample type is required for this test?
How long does it take to get results?
Is home sample collection available?
What is the turnaround time for outstation samples?
Can this test be used for cancer diagnosis?
Is fasting required before sample collection?
What is the difference between 2x250 and 2x150 sequencing?
Are there any additional charges for data analysis?
Can I get a discount for multiple samples?
Is this test NABL accredited?
How should I interpret my results?
Related Tests
Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
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