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Alkaline Phosphatase (ALP) Isoenzymes Test

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Alkaline Phosphatase (ALP) Isoenzymes Test

Short Name: ALP Isoenzymes Test

Also known as: ALP Isoforms Test, Alkaline Phosphatase Fractionation

Alkaline Phosphatase (ALP) Isoenzymes Test test available at DNA Labs India for ₹8,000. Uses Agarose Gel Electrophoresis on Serum samples. Results in Next day. Free home collection in 300+ cities across India.

Specialized Diagnostic Test🏠 Home Collection

🩺 Medically Reviewed By

Overview

To measure and differentiate between various forms of alkaline phosphatase in the blood for diagnosing liver and bone disorders.

Test Code
76
Price
₹8,000
Sample Type
Serum
Result Time
Next day
Fasting Required
Yes (8 hours)
Method
Agarose Gel Electrophoresis
Step 1

Sample Collection

Fast overnight (8-12 hours) before sample collection. Inform your doctor about any medications.

Method: Venipuncture

Step 2

Laboratory Analysis

A blood sample will be drawn from a vein in your arm by a trained phlebotomist.

Step 3

Report Delivery

Apply pressure to the puncture site to stop bleeding. You may resume normal activities.

Timeline: Next day

Patient Instructions

1
Before the Test:Fast overnight (8-12 hours) before sample collection. Inform your doctor about any medications.
2
During the Test:A blood sample will be drawn from a vein in your arm by a trained phlebotomist.
3
After the Test:Apply pressure to the puncture site to stop bleeding. You may resume normal activities.

About This Test

Who Should Get This Test

To measure and differentiate between various forms of alkaline phosphatase in the blood for diagnosing liver and bone disorders.

How to Prepare

  • Collect 2 mL serum in SST tube
  • Avoid hemolysis during collection
  • Ship refrigerated or frozen to lab

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"This test is crucial for differentiating between liver and bone disorders. Consult your doctor if you have symptoms like jaundice or bone pain for accurate diagnosis and treatment."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeSerum
Sample Volume2 mL (1.5 mL min.)
ContainerSST (Serum Separator Tube)
Collection MethodVenipuncture

Sample Stability

Room Temperature4 hours
Refrigerator48 hours
Frozen4 weeks
Sample Rejection Criteria:
  • Hemolyzed samples
  • Insufficient sample volume
  • Improper storage or handling

Understanding Your Results

Interpretation of ALP Isoenzymes Test results involves analyzing the pattern of isoenzymes to identify the source of elevated ALP.
📊

High liver ALP

📊

High bone ALP

📊

Mixed pattern

⚠️ When to Consult a Doctor:

If you have symptoms like jaundice, bone pain, or abnormal test results, consult a gastroenterologist or rheumatologist for further evaluation.

Limitations

  • May not distinguish between all causes of elevated ALP
  • Requires skilled interpretation for accurate diagnosis

Risks & Considerations

  • Pain at puncture site
  • Bruising
  • Infection (rare)

Interfering Factors

  • Hemolysis of sample
  • Recent high-fat meal
  • Certain medications like antibiotics or anticonvulsants

Compare With Similar Tests

TestAlkaline Phosphatase (ALP) Isoenzymes TestLiver Function Test (LFT)Bone Profile Test
ComparisonAlkaline Phosphatase (ALP) Isoenzymes Test

Frequently Asked Questions

What is the Alkaline Phosphatase (ALP) Isoenzymes Test?
It is a blood test that measures different forms of the ALP enzyme to help diagnose liver and bone disorders.
Why is the ALP Isoenzymes Test recommended?
It is recommended to differentiate between liver and bone issues when ALP levels are elevated or symptoms are present.
What symptoms indicate the need for this test?
Symptoms like jaundice, abdominal pain, bone pain, fractures, dark urine, or fatigue may prompt this test.
How is the test performed?
A blood sample is drawn from your arm and analyzed using agarose gel electrophoresis to separate ALP isoenzymes.
What is the cost of the ALP Isoenzymes Test at DNA Labs India?
The test costs INR 8000 with free home sample collection across India.
Is fasting required before the test?
Yes, overnight fasting (8-12 hours) is preferred for accurate results.
How long does it take to get the test results?
Results are typically available the next day after sample collection.
What do high levels of ALP isoenzymes mean?
High levels can indicate liver disorders like hepatitis or bone conditions like osteoporosis, depending on the isoenzyme type.
Are there any risks associated with the test?
Risks are minimal and include pain at the puncture site, bruising, or rare infection.
Can this test detect bone disorders?
Yes, by identifying elevated bone-type ALP, it can help diagnose bone disorders like fractures or osteoporosis.
How is this test different from a standard ALP test?
This test separates ALP into isoenzymes to pinpoint the source, while a standard ALP test measures total levels only.
Where can I get the ALP Isoenzymes Test done in India?
At DNA Labs India, with home collection available in numerous cities across India. Book online for convenience.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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