Anti A Titre IgG Test
Short Name: Anti-A Titre IgG
Also known as: Anti-A IgG Antibody Titre, IgG Anti-A Titre, ABO Antibody Titre IgG, Anti A Antibody Test IgG
Anti A Titre IgG Test test available at DNA Labs India for ₹1,170. Uses Erythrocyte Magnetized Technology (EMT) on Serum from SST tube and Whole Blood from EDTA tube samples. Results in Sample collected daily by 9:00 AM. Report available by the next day via online portal, email, or WhatsApp.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The primary purpose of the Anti A Titre IgG test is to measure and quantify IgG-class antibodies against the A blood group antigen. This measurement is clinically important for assessing the risk of ABO-incompatible hemolytic transfusion reactions and for monitoring pregnancies where ABO incompatibility between mother and fetus may lead to hemolytic disease of the fetus and newborn (HDFN). The test helps clinicians make informed decisions regarding blood product selection, pregnancy surveillance, and neonatal management.
- Test Code
- 141
- CPT Code
- 86886
- ICD Code
- O36.09
- Price
- ₹1,170
- Sample Type
- Serum from SST tube and Whole Blood from EDTA tube
- Result Time
- Sample collected daily by 9:00 AM. Report available by the next day via online portal, email, or WhatsApp.
- Fasting Required
- No
- Method
- Erythrocyte Magnetized Technology (EMT)
Sample Collection
No special preparation or fasting is required. Inform the phlebotomist about any recent blood transfusions, immunoglobulin therapy, or current medications that may affect immune function.
Method: Venipuncture
Laboratory Analysis
A trained phlebotomist will collect blood via venipuncture. Two tubes are required: a serum separator tube (SST) for serum and a Lavender Top (EDTA) tube for whole blood. The collection process typically takes 5 to 10 minutes.
Report Delivery
Apply gentle pressure to the puncture site with a cotton ball or gauze for 3 to 5 minutes. Avoid heavy lifting with the affected arm for a few hours. Mild bruising at the site is normal and resolves on its own.
Timeline: Sample collected daily by 9:00 AM. Report available by the next day via online portal, email, or WhatsApp.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the Anti A Titre IgG test is to measure and quantify IgG-class antibodies against the A blood group antigen. This measurement is clinically important for assessing the risk of ABO-incompatible hemolytic transfusion reactions and for monitoring pregnancies where ABO incompatibility between mother and fetus may lead to hemolytic disease of the fetus and newborn (HDFN). The test helps clinicians make informed decisions regarding blood product selection, pregnancy surveillance, and neonatal management.
How to Prepare
- Collect 2 mL (1 mL minimum) of serum from 1 SST tube
- Collect 3 mL (2 mL minimum) of whole blood in 1 Lavender Top (EDTA) tube
- Ship samples refrigerated at 2 to 8 degrees Celsius
- Do NOT freeze the samples at any point
- Label all tubes clearly with patient name, date of collection, and sample type
- Ensure samples reach the laboratory within the specified stability window
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"In clinical practice, the Anti A Titre IgG test is indispensable when managing pregnancies at risk of ABO hemolytic disease. When a mother with blood group B or O carries a fetus with blood group A, IgG anti-A antibodies produced by the mother can cross the placenta and potentially cause hemolysis of fetal red blood cells. Serial monitoring of antibody titres helps assess the risk of hemolytic disease of the fetus and newborn (HDFN). In the transfusion medicine setting, knowledge of a patient's IgG anti-A titre is critical for safe blood product selection, especially in patients with prior sensitization through transfusion or pregnancy. I recommend this test for any patient with a history of adverse transfusion reactions, sensitized pregnancies, or when ABO-incompatible transplantation is being considered. Early detection and monitoring allow timely clinical intervention and can significantly improve patient outcomes."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed serum sample
- Lipemic or severely icteric sample
- Insufficient sample volume below the minimum requirement
- Improperly labeled or unlabeled tubes
- Frozen sample
- Sample received beyond the 72-hour refrigerated stability window
- Clotted sample in the EDTA tube
Understanding Your Results
No clinically significant IgG anti-A antibodies detected. Low or absent risk of ABO-mediated hemolysis in transfusion or pregnancy settings.
Status: Normal
Range: Negative (< 1:16)
Low levels of IgG anti-A antibodies present. May indicate early sensitization. Clinical correlation is recommended, and repeat testing may be advised in pregnancy.
Status: Borderline
Range: Low Titre (1:16 to 1:64)
Clinically significant levels of IgG anti-A antibodies detected. In pregnancy, this may indicate an increased risk of ABO hemolytic disease of the fetus and newborn. In transfusion settings, specialized blood product selection may be required. Clinical consultation and monitoring are strongly recommended.
Status: Elevated
Range: High Titre (≥ 1:64)
Consult your doctor if your Anti-A IgG titre is elevated (≥ 1:64), especially during pregnancy. Your doctor may recommend serial monitoring, Doppler assessments, or neonatal management planning. If you have experienced symptoms such as fever, chills, back pain, blood in urine, or jaundice following a blood transfusion, seek immediate medical attention as these may indicate a hemolytic transfusion reaction.
Limitations
- ⚠The test measures antibody titre but cannot independently predict the severity of hemolytic disease in the fetus or newborn
- ⚠A single titre measurement reflects a snapshot in time; serial measurements may be needed for trend assessment
- ⚠Results should always be interpreted in conjunction with clinical history, blood grouping, and other relevant laboratory findings
- ⚠IgM anti-A antibodies may co-exist and, if not properly separated, may affect IgG titre measurement
- ⚠The test does not differentiate between subclasses of IgG antibodies (e.g., IgG1, IgG3), which may have different clinical significance
Risks & Considerations
- ●Minor bruising or hematoma at the venipuncture site
- ●Slight pain or discomfort during needle insertion
- ●Rare risk of infection at the puncture site
- ●Very rare risk of fainting or dizziness during blood draw
Interfering Factors
- ●Recent blood transfusion within the past 3 months may alter antibody levels and produce misleading results
- ●Hemolyzed or lipemic serum samples may interfere with test accuracy
- ●Recent administration of intravenous immunoglobulin (IVIG) may cause false elevation
- ●Administration of anti-D immunoglobulin may produce cross-reactive interference
- ●Certain medications that affect immune function may influence antibody production
Compare With Similar Tests
| Test | Anti A Titre IgG Test | Anti B Titre IgG Test | Indirect Antiglobulin Test (IAT) | Direct Antiglobulin Test (DAT) |
|---|---|---|---|---|
| Comparison | Anti A Titre IgG Test | While the Anti A Titre IgG test detects IgG antibodies against the A antigen, the Anti B Titre IgG test measures IgG antibodies against the B antigen. Both tests are relevant for ABO incompatibility assessment but target different antigens. They are often ordered together for comprehensive evaluation. | The IAT detects the presence of unexpected antibodies in the patient's serum, including antibodies to ABO and non-ABO antigens. The Anti A Titre IgG test is more specific, quantifying only IgG antibodies against the A antigen. IAT is used as a screening test, while Anti-A IgG titre is a targeted quantification. | DAT detects antibodies or complement bound to the patient's red blood cells in vivo, indicating active hemolysis. In contrast, the Anti A Titre IgG test measures circulating free antibodies in the serum against the A antigen. DAT is useful for diagnosing ongoing hemolysis, while Anti-A IgG titre assesses sensitization risk. |
Frequently Asked Questions
What is the Anti A Titre IgG test?
Why is the Anti A Titre IgG test important during pregnancy?
Do I need to fast before the Anti A Titre IgG test?
How is the sample collected for this test?
What do elevated Anti-A IgG levels indicate?
What is the difference between IgG and IgM anti-A antibodies?
Who should get the Anti A Titre IgG test done?
What is the cost of the Anti A Titre IgG test at DNA Labs India?
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Is the Anti A Titre IgG test covered under insurance or government schemes?
What happens if my Anti-A IgG titre is high?
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