Cattle Genome Sequencing and Variant Calling-10X Test
Short Name: Cattle Genome Seq 10X
Also known as: Bovine Whole Genome Sequencing, Cattle WGS with Variant Calling
Cattle Genome Sequencing and Variant Calling-10X Test test available at DNA Labs India for ₹225,000. Uses Next-Generation Sequencing (NGS), Whole Genome Sequencing (WGS) at 10X coverage, Bioinformatics variant calling pipeline on Extracted DNA samples. Results in Results are typically available within 8 weeks from the date of sample receipt. You will be notified via email or SMS when the report is ready.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The primary purpose of Cattle Genome Sequencing and Variant Calling is to provide a complete genetic blueprint of an individual animal. This enables the identification of beneficial and deleterious genetic variants that influence economically important traits. By understanding the genetic makeup, breeders can select animals with superior genetics for breeding programs, reduce the incidence of hereditary diseases, and optimize herd management strategies. The test also supports research initiatives aimed at improving cattle health and productivity.
- Test Code
- 6346
- CPT Code
- 81435
- ICD Code
- Z13.9
- Price
- ₹225,000
- Sample Type
- Extracted DNA
- Result Time
- Results are typically available within 8 weeks from the date of sample receipt. You will be notified via email or SMS when the report is ready.
- Fasting Required
- No
- Method
- Next-Generation Sequencing (NGS), Whole Genome Sequencing (WGS) at 10X coverage, Bioinformatics variant calling pipeline
Sample Collection
Ensure the animal is properly restrained. For blood collection, use sterile equipment. For hair samples, pull hair follicles from the tail or mane. For tissue biopsy, follow veterinary guidance. Avoid contamination by wearing gloves.
Method: Blood, hair follicles, or tissue biopsy
Laboratory Analysis
Collect the sample as per instructions. For blood, fill the EDTA tube to the indicated mark. For hair, ensure follicles are present. For tissue, place in a sterile container with appropriate preservative.
Report Delivery
Label the sample with animal ID and date. Store blood at 2-8°C if shipping within 24 hours, otherwise freeze. Hair and tissue samples can be stored at room temperature for short periods but should be shipped promptly.
Timeline: Results are typically available within 8 weeks from the date of sample receipt. You will be notified via email or SMS when the report is ready.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of Cattle Genome Sequencing and Variant Calling is to provide a complete genetic blueprint of an individual animal. This enables the identification of beneficial and deleterious genetic variants that influence economically important traits. By understanding the genetic makeup, breeders can select animals with superior genetics for breeding programs, reduce the incidence of hereditary diseases, and optimize herd management strategies. The test also supports research initiatives aimed at improving cattle health and productivity.
How to Prepare
- Blood: Collect 5-10 mL in EDTA tube, mix gently to prevent clotting.
- Hair: Pluck 20-30 hair follicles with roots, place in a clean envelope or tube.
- Tissue: Obtain a small biopsy (ear notch) and place in a sterile tube with DNA stabilizer.
- Ship samples to the lab within 48 hours; use ice packs for blood samples.
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"Genomic sequencing in cattle enables precision breeding, early disease risk assessment, and improved herd management. This comprehensive 10X coverage test provides high-confidence variant calls for actionable insights."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed blood samples
- Insufficient DNA quantity or quality
- Improper labeling or missing sample ID
- Samples received after prolonged storage without proper preservation
Understanding Your Results
Animal is a carrier. Breeding with another carrier may produce affected offspring. Avoid mating with carriers.
Recommendation: Select non-carrier mates to eliminate the risk of affected progeny.
Elevated inbreeding levels, which may reduce fertility and increase genetic disorders.
Recommendation: Introduce unrelated genetics to increase diversity.
Animal carries alleles associated with improved milk yield or composition.
Recommendation: Use in breeding programs to enhance these traits.
No variants associated with common hereditary diseases were found.
Recommendation: Continue routine health monitoring and maintain good management practices.
If you are a livestock owner or breeder, consult with a veterinary geneticist or animal breeding specialist to discuss the results and their implications for your herd. For research applications, consult with a genomics expert for detailed interpretation.
Limitations
- ⚠10X coverage may not detect all structural variants or low-level mosaicism
- ⚠Variant interpretation is based on current knowledge; some variants may be of unknown significance
- ⚠Not a substitute for clinical diagnosis of infectious diseases
- ⚠Results are for research and breeding purposes; not intended for human consumption decisions
- ⚠Requires high-quality DNA; degraded samples may fail
Risks & Considerations
- ●Minimal risk of infection at the sample collection site
- ●Bruising or hematoma at blood collection site
- ●Stress to the animal during handling
Interfering Factors
- ●Poor DNA quality or quantity due to improper sample collection or storage
- ●Contamination with microbial or other animal DNA
- ●Low sequencing coverage (<10X) leading to missed variants
- ●Reference genome mismatches for rare breeds
- ●Bioinformatics pipeline variations affecting variant calling accuracy
Compare With Similar Tests
| Test | Cattle Genome Sequencing and Variant Calling-10X | Cattle Microarray SNP Chip | Targeted Gene Panel (e.g., for specific diseases) | Whole Genome Sequencing (30X) |
|---|---|---|---|---|
| Comparison | Cattle Genome Sequencing and Variant Calling-10X |
Frequently Asked Questions
What is the cost of Cattle Genome Sequencing and Variant Calling-10X at DNA Labs India?
What sample types are accepted for this test?
How long does it take to get the results?
Is home sample collection available?
What is the difference between 10X and 30X genome sequencing?
Can this test identify all genetic diseases in cattle?
Do I need to fast the animal before sample collection?
Will the report include recommendations for breeding?
Is this test suitable for all cattle breeds?
How should I store the sample before shipping?
Can this test be used for research purposes?
What is the accuracy of the variant calling?
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