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Chlamydia Trachomatis and Neisseria Gonorrhoeae PCR Qualitative Test

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Chlamydia Trachomatis and Neisseria Gonorrhoeae PCR Qualitative Test

Short Name: CT/NG PCR Qualitative

Also known as: CT/NG PCR Test, Chlamydia Gonorrhea NAAT Test, STI Dual Panel PCR Test, Chlamydia Gonorrhea DNA Test, Dual STI PCR Screen

Chlamydia Trachomatis and Neisseria Gonorrhoeae PCR Qualitative Test test available at DNA Labs India for ₹6,500. Uses Real Time PCR, Nucleic Acid Amplification Test (NAAT) on Urine / Vaginal Swab / Endocervical Swab / Conjunctival Swab samples. Results in Sample daily by 11:00 AM; Report available next day. Results are delivered via Online Portal, Email, and WhatsApp.. Free home collection in 300+ cities across India.

PCR Qualitative15 years and above🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Kothari Ramesh Shantilal

Dr Kothari Ramesh Shantilal

Consultant Microbiologist · Reg: 27670

Last reviewed: September 7, 2026

Overview

This test is used for the qualitative detection and differentiation of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acid in clinical specimens. It serves as a primary diagnostic tool for confirming active infections caused by these two organisms, which are the leading bacterial causes of sexually transmitted infections worldwide. The test aids physicians in initiating appropriate antibiotic therapy and helps in contact tracing and partner notification to prevent further transmission.

Test Code
308
CPT Code
87491, 87591
ICD Code
A56.0, A54.0
Price
₹6,500
Sample Type
Urine / Vaginal Swab / Endocervical Swab / Conjunctival Swab
Result Time
Sample daily by 11:00 AM; Report available next day. Results are delivered via Online Portal, Email, and WhatsApp.
Fasting Required
No
Method
Real Time PCR, Nucleic Acid Amplification Test (NAAT)
Step 1

Sample Collection

Avoid antibiotics for at least 2 weeks prior to testing unless directed otherwise by your physician. First morning urine sample is preferred for highest accuracy. For urine collection, do not urinate for at least 1-2 hours before providing the sample. Avoid using douches, vaginal creams, antiseptics, or lubricants 24 hours before sample collection. Inform your healthcare provider about any medications currently being taken.

Method: First morning urine collection or sterile swab collection by healthcare professional

Step 2

Laboratory Analysis

For urine sample: Clean the genital area with the antiseptic wipe provided in the kit. Collect the first 15-30 mL of the urine stream in a sterile screw-capped container. Do not collect midstream urine. For swab collection: A qualified healthcare professional will collect the sample using a sterile swab from the vagina, endocervix, or conjunctiva as clinically indicated. The swab will be placed in normal saline for transport.

Step 3

Report Delivery

Ensure the sample container is properly sealed and labeled with your name, date, and time of collection. Store the sample refrigerated if it cannot be transported immediately. The sample will be transported to the laboratory under appropriate cold chain conditions. Results will be available within 24 hours of the sample reaching the laboratory. You can access your reports online through the DNA Labs India portal, email, or WhatsApp.

Timeline: Sample daily by 11:00 AM; Report available next day. Results are delivered via Online Portal, Email, and WhatsApp.

Patient Instructions

1
Before the Test:No fasting is required before this test. A first morning urine sample is preferred for optimal accuracy. Avoid urinating for at least 1-2 hours before providing the urine sample. Discontinue antibiotics at least 2 weeks before testing unless instructed otherwise by your physician. Avoid douching, using vaginal creams, or applying antiseptics near the collection site 24 hours before the test. Inform your doctor about all current medications.
2
During the Test:The sample collection process is simple and painless for urine samples. You will be asked to provide the first 15-30 mL of your urine stream in a sterile container. For swab samples, a healthcare professional will gently collect the specimen from the appropriate site (vagina, endocervix, or conjunctiva). The entire collection process takes only a few minutes.
3
After the Test:After sample collection, the specimen is sealed, labeled, and transported to the laboratory under controlled temperature conditions. You may resume normal activities immediately. Results are typically available within 24 hours and can be accessed through the DNA Labs India online portal, via email, or through WhatsApp. If results are positive, consult your physician for treatment without delay.

About This Test

Who Should Get This Test

This test is used for the qualitative detection and differentiation of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acid in clinical specimens. It serves as a primary diagnostic tool for confirming active infections caused by these two organisms, which are the leading bacterial causes of sexually transmitted infections worldwide. The test aids physicians in initiating appropriate antibiotic therapy and helps in contact tracing and partner notification to prevent further transmission.

How to Prepare

  • First morning urine sample is preferred for highest test accuracy
  • Collect at least 8 mL of urine in a 15 mL sterile screw-capped container
  • For swab samples, use only the sterile swab kit provided by the laboratory
  • Do not touch or contaminate the inside of the container or swab tip
  • Label the sample clearly with your full name, date, and time of collection
  • Store the sample refrigerated (2-8°C) if not being transported immediately
  • For female patients, avoid collecting vaginal swab samples during menstruation if possible

Doctor's Notes

Reviewed by — MBBS, MD (Microbiology) · Reg. No. 27670

"As an infectious disease specialist, I strongly recommend PCR-based testing for Chlamydia trachomatis and Neisseria gonorrhoeae as it offers the highest sensitivity and specificity among available diagnostic methods. These infections are frequently asymptomatic, and undiagnosed cases can lead to severe reproductive health complications including pelvic inflammatory disease, tubal infertility, and ectopic pregnancy. The dual-panel PCR test is particularly valuable as co-infection with both organisms is common. Early detection through this test enables timely antibiotic treatment, prevents transmission to sexual partners, and significantly reduces the risk of long-term health consequences. I recommend routine screening for all sexually active individuals, especially those with multiple partners or a history of STIs."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeUrine / Vaginal Swab / Endocervical Swab / Conjunctival Swab
Sample Volume15 mL (8 mL minimum)
ContainerSterile screw-capped container for urine; Swab in normal saline for swab specimens
Collection MethodFirst morning urine collection or sterile swab collection by healthcare professional

Sample Stability

Room Temperature8 hours
Refrigerated (2-8°C)1 week
Frozen (-20°C)4 weeks
Sample Rejection Criteria:
  • Unlabeled or improperly labeled samples
  • Samples received beyond the recommended stability period
  • Insufficient sample volume (less than 8 mL urine)
  • Leaking or visibly contaminated containers
  • Specimens collected with antiseptic preservatives
  • Samples transported without appropriate temperature control

Understanding Your Results

The Chlamydia Trachomatis and Neisseria Gonorrhoeae PCR Qualitative Test detects the presence or absence of bacterial DNA from each organism. A 'Detected' result for either organism indicates active infection requiring medical treatment. A 'Not Detected' result for both organisms indicates no evidence of infection at the time of testing. Results should always be interpreted in the context of clinical symptoms, sexual history, and exposure risk by a qualified healthcare provider.
📊

CT: Not Detected, NG: Not Detected

Normal

📊

CT: Detected, NG: Not Detected

Abnormal

📊

CT: Not Detected, NG: Detected

Abnormal

📊

CT: Detected, NG: Detected

Abnormal

📊

Indeterminate / Invalid

Borderline

⚠️ When to Consult a Doctor:

Consult your doctor immediately if your test results are positive for either Chlamydia trachomatis or Neisseria gonorrhoeae. You should also consult a physician if you experience symptoms such as painful urination, abnormal genital discharge, pelvic or abdominal pain, pain during intercourse, bleeding between periods, or testicular pain. Sexual partners should be notified, tested, and treated to prevent reinfection and further transmission. If you are pregnant and test positive, prompt treatment is essential to prevent neonatal complications.

Limitations

  • ⚠This is a qualitative test and does not quantify the bacterial load
  • ⚠Cannot determine antibiotic susceptibility or resistance patterns
  • ⚠May not distinguish between viable and non-viable organisms after recent treatment
  • ⚠False-negative results are possible in very early infections with low bacterial DNA levels
  • ⚠Cannot detect co-infections with other STI pathogens unless specifically ordered
  • ⚠Cross-reactivity with other Neisseria species may rarely occur, though modern assays have excellent specificity

Risks & Considerations

  • ●Minimal to no discomfort during urine sample collection
  • ●Slight discomfort possible during swab collection from the genital area
  • ●No significant risks associated with this non-invasive diagnostic test
  • ●Rare possibility of false-negative results in very early infections with low bacterial DNA
  • ●Extremely rare risk of sample contamination during self-collection

Interfering Factors

  • ●Recent antibiotic use (within 2 weeks) may suppress bacterial DNA and cause false-negative results
  • ●Use of vaginal douches, creams, or antiseptics within 24 hours before sample collection
  • ●Improper sample collection technique or insufficient sample volume
  • ●Contamination of the specimen during collection or transport
  • ●Urine sample collected too long after the first morning void may have reduced sensitivity
  • ●Menstrual blood in endocervical swab samples may contain PCR inhibitors

Compare With Similar Tests

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Frequently Asked Questions

What is the Chlamydia Trachomatis and Neisseria Gonorrhoeae PCR Qualitative Test?
This is a molecular diagnostic test that uses Real Time PCR (Polymerase Chain Reaction) technology to detect the DNA of Chlamydia trachomatis and Neisseria gonorrhoeae bacteria in urine or swab samples. It is recognized as the gold standard method for diagnosing these sexually transmitted infections due to its high sensitivity (>99%) and specificity (>99%). The test provides a qualitative result, indicating whether each organism is detected or not detected.
Why is PCR testing preferred over other methods for STI detection?
PCR testing is preferred because it offers superior sensitivity and specificity compared to traditional culture methods, antigen tests, or serological tests. PCR can detect very small amounts of bacterial DNA, making it capable of identifying infections even in early stages or in asymptomatic individuals. Additionally, PCR provides faster results (typically within 24 hours) compared to culture methods which may take 3-7 days. The dual-panel format allows simultaneous detection of both Chlamydia and Gonorrhea from a single sample.
What sample types are accepted for this test?
The test accepts multiple sample types: first morning urine (preferred, minimum 8 mL in a sterile screw-capped container), vaginal swab, endocervical swab, or conjunctival swab. Swab samples are collected in normal saline. Urine samples are generally preferred due to their non-invasive nature and ease of collection. Your healthcare provider will recommend the most appropriate sample type based on your clinical situation.
Is fasting required before taking this test?
No, fasting is not required for this test. However, a first morning urine sample is preferred for optimal accuracy. If providing a urine sample, you should avoid urinating for at least 1-2 hours before collection to ensure adequate concentration of bacterial DNA in the specimen. For swab samples, no special preparation is needed beyond avoiding douches and vaginal creams for 24 hours before collection.
How long does it take to get the results?
Results are typically available within 24 hours from the time the sample reaches the laboratory. If your sample is collected before 11:00 AM, you can expect your report by the next day. Results are delivered through multiple channels including the DNA Labs India online portal, email, and WhatsApp for your convenience.
What does a positive test result mean?
A positive result means that the DNA of Chlamydia trachomatis, Neisseria gonorrhoeae, or both has been detected in your sample, indicating an active infection. This requires prompt medical treatment with appropriate antibiotics. Your sexual partner(s) should also be notified, tested, and treated to prevent reinfection and further spread. It is important to consult your physician immediately and complete the full course of prescribed treatment.
What does a negative test result mean?
A negative result means that no DNA from Chlamydia trachomatis or Neisseria gonorrhoeae was detected in your sample. This generally indicates no active infection at the time of testing. However, if testing was performed very soon after potential exposure (within the window period), a repeat test after 2 weeks may be recommended. If you continue to experience symptoms despite a negative result, consult your physician for further evaluation.
Can this test be performed with home sample collection?
Yes, DNA Labs India offers free home sample collection for this test across major cities in India. A trained phlebotomist or healthcare professional will visit your home to collect the sample. For urine samples, you will be provided with a sterile collection kit. For swab samples, the healthcare professional will perform the collection at your home. This ensures convenience, privacy, and comfort during the sample collection process.
How accurate is the PCR test for Chlamydia and Gonorrhea?
The PCR (Real Time PCR) test is considered the most accurate method for detecting Chlamydia trachomatis and Neisseria gonorrhoeae, with both sensitivity and specificity exceeding 99%. This means the test has an extremely low rate of false positives and false negatives. PCR technology can detect even very small quantities of bacterial DNA, making it effective for diagnosing infections in asymptomatic individuals and in early-stage infections where bacterial counts may be low.
Who should get tested for Chlamydia and Gonorrhea?
Testing is recommended for all sexually active individuals, particularly those with multiple sexual partners, a history of STIs, or unprotected sexual intercourse. The CDC recommends annual Chlamydia and Gonorrhea screening for all sexually active women under 25 years of age and for women over 25 with new or multiple sexual partners. Men who have sex with men (MSM) should also be screened annually. Testing is also recommended during pregnancy, before gynecological procedures, and for anyone experiencing symptoms of an STI.
Can I have Chlamydia or Gonorrhea without any symptoms?
Yes, both Chlamydia and Gonorrhoeae infections are often called 'silent infections' because the majority of infected individuals do not experience any symptoms. Approximately 70-80% of women and 50% of men with Chlamydia infections are asymptomatic. Similarly, many Gonorrhea infections may present without noticeable symptoms. Despite the absence of symptoms, these infections can still cause significant damage to the reproductive system and can be transmitted to sexual partners. This is why regular screening is so important.
What are the complications of untreated Chlamydia and Gonorrhea infections?
Untreated Chlamydia and Gonorrhea infections can lead to serious and potentially permanent health complications. In women, these include pelvic inflammatory disease (PID), chronic pelvic pain, tubal factor infertility, and ectopic pregnancy. In men, complications include epididymitis, urethral stricture, and rarely, infertility. Both infections increase the risk of acquiring and transmitting HIV. In pregnant women, these infections can cause premature delivery, neonatal conjunctivitis, and pneumonia in newborns. Early detection and treatment through PCR testing can prevent all of these complications.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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