ddRAD Library Preparation Test
Short Name: ddRAD Library Prep
Also known as: Double-Digest Restriction Site-Associated DNA Library Preparation, ddRAD-seq Library Preparation
ddRAD Library Preparation Test test available at DNA Labs India for ₹145,000. Uses Double-digest restriction site-associated DNA sequencing, Next-Generation Sequencing (NGS) on Extracted DNA samples. Results in Reports are delivered within 1 week after sample submission.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The primary purpose of ddRAD library preparation is to generate a reduced-representation sequencing library that targets a subset of the genome, enabling cost-effective genotyping of many individuals. It is used for SNP discovery, population structure analysis, linkage mapping, and phylogenetic studies. By focusing on specific restriction sites, ddRAD reduces genome complexity, making it ideal for non-model organisms and large-scale studies.
- Test Code
- 6371
- CPT Code
- Not Applicable
- ICD Code
- Not Applicable
- Price
- ₹145,000
- Sample Type
- Extracted DNA
- Result Time
- Reports are delivered within 1 week after sample submission.
- Fasting Required
- No
- Method
- Double-digest restriction site-associated DNA sequencing, Next-Generation Sequencing (NGS)
Sample Collection
Ensure that the DNA sample is extracted using a reliable method and is free from contaminants. If providing blood or tissue, follow the standard collection procedures. No fasting is required.
Method: Blood or tissue sample for DNA extraction (performed by lab or provided by client)
Laboratory Analysis
The sample (extracted DNA) should be transported on ice to maintain stability. If blood is collected, use EDTA or citrate tubes to prevent clotting.
Report Delivery
The sample will be processed in the laboratory. Results will be available within one week. You will be notified via email or WhatsApp when the report is ready.
Timeline: Reports are delivered within 1 week after sample submission.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of ddRAD library preparation is to generate a reduced-representation sequencing library that targets a subset of the genome, enabling cost-effective genotyping of many individuals. It is used for SNP discovery, population structure analysis, linkage mapping, and phylogenetic studies. By focusing on specific restriction sites, ddRAD reduces genome complexity, making it ideal for non-model organisms and large-scale studies.
How to Prepare
- Use sterile, DNase-free tubes for DNA samples
- Label the tube with patient ID and date
- Ship the sample in a cold pack to maintain integrity
- Avoid repeated freeze-thaw cycles
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"ddRAD library preparation is a robust method for genome-wide SNP discovery and genotyping. Proper quality control at each step is essential to ensure reliable downstream sequencing data."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Highly degraded DNA (fragment size < 1 kb)
- DNA concentration < 10 ng/µL
- Visible contamination (e.g., protein, RNA)
- Sample not labeled properly
Understanding Your Results
A ratio of 1.8-2.0 indicates pure DNA. Lower values suggest protein contamination; higher values may indicate RNA contamination.
A concentration ≥ 50 ng/µL is required for efficient library preparation. Lower concentrations may lead to low library yield.
A concentration ≥ 10 nM is optimal for sequencing. Lower concentrations may require additional amplification.
Fragment size of 300-500 bp is ideal for Illumina sequencing. Deviations may affect sequencing quality.
≥ 90% efficiency ensures that most fragments have adapters, reducing the risk of chimeric reads.
≥ 100 ng yield indicates successful amplification. Lower yields may require optimization.
This test is typically requested by researchers or clinicians for genetic studies. If you are a patient, consult your physician or geneticist to understand if this test is appropriate for your condition.
Limitations
- ⚠Requires high-quality, high-molecular-weight DNA
- ⚠Not suitable for samples with very low DNA concentration
- ⚠Bioinformatics analysis may require specialized expertise
- ⚠Limited to organisms with known restriction site patterns
- ⚠May not capture all genomic regions of interest
Risks & Considerations
- ●No direct physical risks as this is a laboratory test
- ●Potential for sample loss or contamination if not handled properly
Interfering Factors
- ●Degraded or sheared DNA
- ●Presence of PCR inhibitors (e.g., heme, humic acid)
- ●Contamination with RNA or proteins
- ●Incomplete digestion due to improper enzyme activity
- ●Adapter contamination or primer dimers
Compare With Similar Tests
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Frequently Asked Questions
What is ddRAD library preparation?
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