ddRAD Sequencing and Primary Analysis-96 Samples Test
Short Name: ddRAD Sequencing (96 Samples)
Also known as: ddRAD-seq, Double Digest Restriction-Site Associated DNA Sequencing
ddRAD Sequencing and Primary Analysis-96 Samples Test test available at DNA Labs India for ₹480,000. Uses Next-Generation Sequencing (NGS), Illumina HiSeq Platform on Extracted DNA samples. Results in Results are typically delivered within 8 weeks after sample receipt. You will be notified via email or SMS when the report is ready.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The purpose of ddRAD sequencing and primary analysis is to identify genetic variants across the genome in a cost-effective manner. It is used for: - Population genetics studies - Linkage mapping - Phylogenetics - Conservation genetics - Identifying disease-associated variants - Agricultural and livestock breeding programs
- Test Code
- 6354
- CPT Code
- 81479
- ICD Code
- Z01.89
- Price
- ₹480,000
- Sample Type
- Extracted DNA
- Result Time
- Results are typically delivered within 8 weeks after sample receipt. You will be notified via email or SMS when the report is ready.
- Fasting Required
- No
- Method
- Next-Generation Sequencing (NGS), Illumina HiSeq Platform
Sample Collection
No special preparation required. If providing blood or saliva sample for DNA extraction, inform about any medications or supplements.
Method: Blood or saliva sample for DNA extraction (if not provided)
Laboratory Analysis
Blood sample collection by trained phlebotomist. For saliva, use provided collection kit.
Report Delivery
No restrictions. Resume normal activities.
Timeline: Results are typically delivered within 8 weeks after sample receipt. You will be notified via email or SMS when the report is ready.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of ddRAD sequencing and primary analysis is to identify genetic variants across the genome in a cost-effective manner. It is used for: - Population genetics studies - Linkage mapping - Phylogenetics - Conservation genetics - Identifying disease-associated variants - Agricultural and livestock breeding programs
How to Prepare
- Provide 2-3 ml blood in EDTA tube or 2 ml saliva in Oragene kit
- If providing extracted DNA, ensure concentration ≥50 ng/µL and total amount ≥500 ng
- Label the tube with patient ID and date
- Ship at room temperature if DNA is stable, or on dry ice for blood/saliva
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"ddRAD sequencing provides a cost-effective approach for genome-wide variant discovery, particularly useful in population genetics and complex trait analysis. The primary analysis ensures high-quality variant calls, essential for downstream interpretation."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed blood samples
- DNA concentration <10 ng/µL
- DNA degraded (high molecular weight smear)
- Improper labeling
- Sample received after prolonged storage at room temperature
Understanding Your Results
SNP
Action: Further validation and functional studies may be required
Indel
Action: Assess impact on protein function
Homozygous vs Heterozygous
Action: Correlate with phenotype
If you have a family history of genetic disorders or are experiencing unexplained symptoms, consult a genetic counselor or physician to discuss the appropriateness of ddRAD sequencing.
Limitations
- ⚠Only detects variants in regions flanking restriction sites
- ⚠May miss variants in repetitive or complex regions
- ⚠Requires high-quality reference genome for accurate mapping
- ⚠Primary analysis does not include clinical interpretation
- ⚠Not suitable for detecting large structural variants
Risks & Considerations
- ●No significant risks associated with blood draw or saliva collection
- ●Minor bruising at blood draw site
- ●Psychological impact of genetic results
Interfering Factors
- ●DNA degradation or contamination
- ●Insufficient DNA quantity
- ●PCR inhibitors
- ●Incorrect restriction enzyme digestion
- ●Index hopping during sequencing
Compare With Similar Tests
| Test | ddRAD Sequencing and Primary Analysis-96 Samples | ||||
|---|---|---|---|---|---|
| Comparison | ddRAD Sequencing and Primary Analysis-96 Samples |
Frequently Asked Questions
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Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
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