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DNA Labs India

ddRAD Sequencing Primary Data Analysis-96 Samples Test

DNA Labs India | ISO 9001:2015 Certified

ddRAD Sequencing Primary Data Analysis-96 Samples Test

Short Name: ddRAD Seq Analysis (96)

Also known as: ddRAD-seq Analysis, Double Digest RAD Sequencing Analysis

ddRAD Sequencing Primary Data Analysis-96 Samples Test test available at DNA Labs India for ₹125,000. Uses ddRAD Sequencing, Primary Data Analysis on Extracted DNA samples. Results in Reports are delivered within 4 weeks from sample receipt.. Free home collection in 300+ cities across India.

Molecular🏠 Home Collection

🩺 Medically Reviewed By

Overview

The purpose of ddRAD sequencing primary data analysis is to process raw sequencing data from 96 samples to identify genetic variants (SNPs) that can be used for various applications such as population structure analysis, linkage mapping, association studies, and marker-assisted selection. This analysis is crucial for ensuring data quality and accuracy before downstream interpretation.

Test Code
6399
CPT Code
81479
ICD Code
Z01.89
Price
₹125,000
Sample Type
Extracted DNA
Result Time
Reports are delivered within 4 weeks from sample receipt.
Fasting Required
No
Method
ddRAD Sequencing, Primary Data Analysis
Step 1

Sample Collection

No specific preparation required. Ensure the sample is collected in a sterile container and transported at appropriate temperature.

Method: Blood or tissue sample for DNA extraction

Step 2

Laboratory Analysis

Sample collection should be performed by trained personnel to avoid contamination.

Step 3

Report Delivery

The sample should be labeled properly and sent to the laboratory within 24 hours if stored at 2-8°C, or within 1 week if frozen at -20°C.

Timeline: Reports are delivered within 4 weeks from sample receipt.

Patient Instructions

1
Before the Test:No special preparation required.
2
During the Test:Sample collection is a simple procedure.
3
After the Test:You can resume normal activities immediately.

About This Test

Who Should Get This Test

The purpose of ddRAD sequencing primary data analysis is to process raw sequencing data from 96 samples to identify genetic variants (SNPs) that can be used for various applications such as population structure analysis, linkage mapping, association studies, and marker-assisted selection. This analysis is crucial for ensuring data quality and accuracy before downstream interpretation.

How to Prepare

  • Use sterile collection tubes
  • Label the tube with patient ID and date
  • Store at 2-8°C for short-term, -20°C for long-term
  • Avoid repeated freeze-thaw cycles

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"ddRAD sequencing is a cost-effective method for genome-wide marker discovery. Primary data analysis ensures high-quality variant calling for downstream applications."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeExtracted DNA
Sample Volume1-2 µg (concentration ≥ 50 ng/µL)
ContainerSterile microcentrifuge tube (1.5 mL)
Collection MethodBlood or tissue sample for DNA extraction

Sample Stability

DNA: stable for 1 week at 2-8°C
DNA: stable for 6 months at -20°C
DNA: stable for 2 years at -80°C
Sample Rejection Criteria:
  • Hemolyzed blood samples
  • Insufficient DNA quantity (< 500 ng)
  • DNA degradation (high molecular weight smear on gel)
  • Improper labeling
  • Sample received after prolonged storage at room temperature

Understanding Your Results

The primary data analysis report provides quality metrics and variant calls. Interpretation should be done by a qualified geneticist or bioinformatician. Variants are reported in VCF format and can be used for downstream analysis.
Check alignment rate and coverage depth to ensure data quality
Review number of variants and transition/transversion ratio for accuracy
Filter variants based on quality scores and depth
Compare with reference genome for annotation
Use population-specific databases for allele frequency
⚠️ When to Consult a Doctor:

If you are using this test for clinical diagnosis, consult a genetic counselor or physician to understand the implications of the results.

Limitations

  • Reduced genome coverage compared to whole-genome sequencing
  • Requires high-quality reference genome for alignment
  • May miss variants in repetitive regions
  • Bioinformatics expertise required for interpretation
  • Not suitable for detecting large structural variants

Risks & Considerations

  • No significant risks associated with sample collection
  • Possible bruising at blood draw site
  • Rare infection at venipuncture site

Interfering Factors

  • Low DNA quality or quantity
  • Contamination with foreign DNA
  • Degraded DNA samples
  • Incomplete restriction digestion
  • PCR duplicates
  • Sequencing errors

Compare With Similar Tests

TestddRAD Sequencing Primary Data Analysis-96 SamplesWhole Genome SequencingddRAD Sequencing
ComparisonddRAD Sequencing Primary Data Analysis-96 Samples

Frequently Asked Questions

What is ddRAD sequencing?
ddRAD sequencing is a reduced-representation sequencing method that uses two restriction enzymes to fragment the genome, allowing cost-effective discovery of thousands of SNPs across many samples.
What does primary data analysis include?
It includes quality trimming, adapter removal, alignment to reference genome, variant calling, and initial filtering to produce high-quality variant calls.
How many samples are covered in this test?
This test covers 96 samples.
What is the cost of this test?
The cost is INR 125,000 for 96 samples.
What is the turnaround time?
Reports are delivered within 4 weeks.
What sample type is required?
Extracted DNA is required. We provide free home sample collection for blood or tissue samples.
Is fasting required?
No, fasting is not required.
Can this test be used for clinical diagnosis?
It is primarily for research purposes, but can be used for clinical applications with proper validation and genetic counseling.
What is the quality of data analysis?
We use advanced bioinformatics pipelines with rigorous quality control to ensure accurate results.
Do you provide home sample collection?
Yes, we offer free home sample collection for online bookings across India.
What are the applications of ddRAD sequencing?
Applications include population genetics, conservation genomics, QTL mapping, and association studies.
How do I book this test?
You can book online through our website or call our customer support.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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