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DNA Labs India

DNA Extraction from Solid Tissue - Plant Test

DNA Labs India | ISO 9001:2015 Certified

DNA Extraction from Solid Tissue - Plant Test

Short Name: Plant DNA Extraction

Also known as: Plant DNA Isolation, Solid Tissue DNA Extraction

DNA Extraction from Solid Tissue - Plant Test test available at DNA Labs India for ₹850. Uses CTAB method, Qiagen DNeasy Plant Mini Kit on Plant tissue (leaf, root, stem, fruit) samples. Results in Reports are delivered within 4 days from sample receipt.. Free home collection in 300+ cities across India.

Molecular🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SHAILAJA RAGHUNATH MURDESHWAR

Dr SHAILAJA RAGHUNATH MURDESHWAR

Consultant Physician · Reg: 8052

Last reviewed: September 7, 2026

Overview

The primary purpose of DNA extraction from solid plant tissue is to obtain pure, intact DNA suitable for various downstream applications, including PCR, sequencing, restriction fragment length polymorphism (RFLP), and next-generation sequencing (NGS). High-quality DNA is essential for accurate genotyping, marker-assisted selection, phylogenetic studies, and detection of plant pathogens. This test is also used in forensic botany and quality control of herbal products.

Test Code
6319
CPT Code
CPT 83891
ICD Code
Z01.89
Price
₹850
Sample Type
Plant tissue (leaf, root, stem, fruit)
Result Time
Reports are delivered within 4 days from sample receipt.
Fasting Required
No
Method
CTAB method, Qiagen DNeasy Plant Mini Kit
Step 1

Sample Collection

Ensure the plant tissue is fresh and free from visible contamination. If possible, collect young, healthy tissue for optimal DNA yield. Place the sample in the provided sterile container with desiccant or silica gel to preserve DNA integrity during transport. Avoid freezing without proper preservation.

Method: Sterile scalpel or punch, placed in provided container

Step 2

Laboratory Analysis

Use sterile scissors or a scalpel to cut approximately 100 mg of tissue. Avoid touching the sample with bare hands. Immediately place the tissue into the collection tube and seal tightly. Label the tube with your details.

Step 3

Report Delivery

Store the sample at room temperature if using desiccant, or refrigerate if processing within 24 hours. Ship the sample to the laboratory as soon as possible. Ensure the sample does not get wet or contaminated.

Timeline: Reports are delivered within 4 days from sample receipt.

Patient Instructions

1
Before the Test:No special preparation is required. However, ensure the plant tissue is collected and stored properly to maintain DNA integrity.
2
During the Test:The sample is processed in the laboratory using validated extraction protocols. The procedure typically takes a few hours, but the full turnaround time is 4 days including quality checks.
3
After the Test:You will receive a report with the DNA concentration, purity, and integrity. The extracted DNA can be stored at -20°C for long-term use.

About This Test

Who Should Get This Test

The primary purpose of DNA extraction from solid plant tissue is to obtain pure, intact DNA suitable for various downstream applications, including PCR, sequencing, restriction fragment length polymorphism (RFLP), and next-generation sequencing (NGS). High-quality DNA is essential for accurate genotyping, marker-assisted selection, phylogenetic studies, and detection of plant pathogens. This test is also used in forensic botany and quality control of herbal products.

How to Prepare

  • Collect 100 mg of plant tissue (leaf, root, stem, or fruit).
  • Use sterile tools to avoid contamination.
  • Place tissue in the provided sterile tube with desiccant.
  • Label the tube with patient/sample ID and date.
  • Transport at room temperature or refrigerated (2-8°C) if delay expected.

Doctor's Notes

Reviewed by — MBBS, MD (General Medicine) · Reg. No. 8052

"High-quality DNA extraction is the cornerstone of reliable genetic testing. Our standardized protocols ensure pure, intact DNA suitable for a wide range of downstream applications."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypePlant tissue (leaf, root, stem, fruit)
Sample Volume100 mg (approx.)
ContainerSterile tube with desiccant or silica gel
Collection MethodSterile scalpel or punch, placed in provided container

Sample Stability

Room temperature (with desiccant)
Refrigerated (2-8°C)
Frozen (-20°C)
Sample Rejection Criteria:
  • Sample received in leaking or broken container
  • Insufficient tissue amount (<50 mg)
  • Sample contaminated with soil or mold
  • Sample without proper labeling
  • Sample stored in inappropriate conditions (e.g., wet, frozen without preservative)

Understanding Your Results

The results of DNA extraction are reported as concentration, purity, and integrity. These parameters indicate the quality and quantity of DNA obtained, which directly impacts the success of downstream applications.
📊

A concentration ≥ 50 ng/µL is generally sufficient for most downstream applications. Lower concentrations may require concentration steps or re-extraction.

📊

A ratio between 1.8 and 2.0 indicates pure DNA. Ratios below 1.8 suggest protein contamination, while ratios above 2.0 may indicate RNA contamination.

📊

A high molecular weight band on agarose gel indicates intact DNA. Smearing suggests degradation, which may affect downstream results.

⚠️ When to Consult a Doctor:

If you are a researcher or clinician planning to use the extracted DNA for genetic testing, it is advisable to consult with a geneticist or molecular biologist to ensure the DNA quality meets the requirements of your specific application. For clinical diagnostics, a physician should interpret the results in the context of the patient's condition.

Limitations

  • ⚠DNA yield may vary depending on plant species and tissue type
  • ⚠Highly fibrous or woody tissues may require additional processing
  • ⚠Degraded DNA may not be suitable for certain downstream applications
  • ⚠The test does not include any downstream analysis (e.g., PCR, sequencing)

Risks & Considerations

  • ●No significant risks are associated with this test as it involves plant tissue only.

Interfering Factors

  • ●Presence of polysaccharides and polyphenols in plant tissue
  • ●Insufficient tissue amount or poor sample preservation
  • ●Contamination with soil or other organic matter
  • ●Excessive mechanical shearing during homogenization

Compare With Similar Tests

TestDNA Extraction from Solid Tissue - Plant
ComparisonDNA Extraction from Solid Tissue - Plant

Frequently Asked Questions

What is DNA extraction from solid tissue?
It is a process to isolate DNA from plant tissues such as leaves, roots, stems, or fruits, removing other cellular components to obtain pure genetic material for analysis.
Why is plant DNA extraction more difficult than animal DNA extraction?
Plant cells have a rigid cell wall made of cellulose, and they contain polysaccharides and polyphenols that can interfere with DNA purity and yield.
What is the cost of DNA extraction from plant tissue at DNA Labs India?
The cost is INR 850 per sample, which includes extraction, purification, and quality assessment.
What types of plant tissues can be used for this test?
We accept leaf, root, stem, and fruit tissues. Young, healthy tissues are recommended for optimal DNA yield.
How much plant tissue is required?
Approximately 100 mg of tissue is required. This is roughly the size of a small leaf or a few root segments.
What is the turnaround time for this test?
Reports are delivered within 4 days from the date of sample receipt.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across major cities in India.
What methods are used for DNA extraction?
We use the CTAB method and Qiagen DNeasy Plant Mini Kit, both of which are validated for high-quality DNA isolation.
How is the quality of extracted DNA assessed?
DNA quality is assessed using spectrophotometry (A260/A280 ratio) and agarose gel electrophoresis to check purity and integrity.
Can the extracted DNA be used for PCR or sequencing?
Yes, the DNA is suitable for PCR, sequencing, genotyping, and other molecular applications, provided it meets the required quality standards.
Are there any special precautions for sample collection?
Use sterile tools, avoid contamination, and place the tissue in the provided container with desiccant. Do not freeze without preservative.
Is this test covered by insurance?
Generally, this test is not covered by insurance as it is often used for research or agricultural purposes. Please check with your provider.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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