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DNA Labs India

DNA Library Preparation-ONT Test

DNA Labs India | ISO 9001:2015 Certified

DNA Library Preparation-ONT Test

Short Name: DNA Library Prep ONT

Also known as: ONT Library Prep, Nanopore Library Preparation, DNA Library Construction for ONT

DNA Library Preparation-ONT Test test available at DNA Labs India for ₹30,000. Uses Oxford Nanopore Technology (ONT), DNA Library Preparation on Extracted DNA samples. Results in Reports are typically available within 12 days after the sample reaches the laboratory.. Free home collection in 300+ cities across India.

Molecular🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

The purpose of DNA library preparation-ONT is to prepare a DNA sample for sequencing using Oxford Nanopore Technology. This process is crucial for generating high-quality sequencing data that can identify genetic variants, mutations, and structural changes associated with various diseases. It enables accurate diagnosis, prognosis, and treatment planning for genetic disorders.

Test Code
6374
CPT Code
0090U
ICD Code
Z01.89
Price
₹30,000
Sample Type
Extracted DNA
Result Time
Reports are typically available within 12 days after the sample reaches the laboratory.
Fasting Required
No
Method
Oxford Nanopore Technology (ONT), DNA Library Preparation
Step 1

Sample Collection

No special preparation is required. However, if a blood or saliva sample is needed for DNA extraction, follow standard collection guidelines.

Method: Saliva or Blood (if extraction required)

Step 2

Laboratory Analysis

The sample collection is non-invasive. For saliva, provide 2 mL in the provided container. For blood, a standard venipuncture will be performed.

Step 3

Report Delivery

No specific aftercare is needed. You can resume normal activities immediately.

Timeline: Reports are typically available within 12 days after the sample reaches the laboratory.

Patient Instructions

1
Before the Test:No specific preparation is needed. However, if you are providing a blood or saliva sample for DNA extraction, follow the instructions provided by our collection team.
2
During the Test:The test involves laboratory processing of your DNA sample. You do not need to be present during the procedure.
3
After the Test:You will receive your report via email or online portal within 12 days. Our genetic counselors are available to discuss the results with you.

About This Test

Who Should Get This Test

The purpose of DNA library preparation-ONT is to prepare a DNA sample for sequencing using Oxford Nanopore Technology. This process is crucial for generating high-quality sequencing data that can identify genetic variants, mutations, and structural changes associated with various diseases. It enables accurate diagnosis, prognosis, and treatment planning for genetic disorders.

How to Prepare

  • Use the provided DNA collection kit for saliva or blood
  • Ensure the sample is labeled with your name and date of birth
  • Store the sample at room temperature if collected at home, and inform the courier for pickup
  • Avoid eating, drinking, or smoking 30 minutes before saliva collection

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"DNA library preparation is a critical step in next-generation sequencing. Proper library quality ensures accurate variant detection, which is essential for diagnosing genetic disorders."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeExtracted DNA
Sample Volume1-2 µg (in 10-20 µL)
ContainerDNA LoBind tube
Collection MethodSaliva or Blood (if extraction required)

Sample Stability

Extracted DNA: Stable at -20°C for up to 6 months
Saliva sample: Stable at room temperature for 7 days
Blood sample: Stable at 2-8°C for 72 hours
Sample Rejection Criteria:
  • Hemolyzed blood samples
  • Insufficient DNA quantity (<500 ng)
  • DNA with high degradation (DIN <5)
  • Sample not labeled correctly

Understanding Your Results

The results of DNA library preparation-ONT are interpreted in the context of the clinical indication. The quality metrics of the library (concentration, fragment size, adapter ligation) are assessed to ensure they meet sequencing standards. Sequencing data is then analyzed to identify genetic variants that may be associated with the patient's condition.
📊

All parameters within reference range

Proceed with sequencing; results are reliable

📊

Concentration <20 ng/µL

May require re-preparation or additional amplification

📊

Fragment size <10 kb

May affect sequencing accuracy; consider re-collection

📊

Efficiency <90%

Sequencing may fail; repeat library preparation

⚠️ When to Consult a Doctor:

Consult a genetic counselor or healthcare professional if you have symptoms suggestive of a genetic disorder, a family history of inherited conditions, or if you are planning a family and have concerns about genetic risks.

Limitations

  • ⚠Requires high-quality extracted DNA; degraded samples may yield poor results
  • ⚠Not a diagnostic test itself; results require clinical correlation
  • ⚠May not detect all types of genetic variants (e.g., large structural variants may be missed depending on coverage)
  • ⚠Bioinformatics analysis may require additional time for complex cases

Risks & Considerations

  • ●No physical risks associated with this test
  • ●Potential psychological impact of genetic results
  • ●Risk of incidental findings

Interfering Factors

  • ●DNA degradation due to improper storage or handling
  • ●Contamination with proteins or RNA
  • ●Insufficient DNA quantity
  • ●Presence of PCR inhibitors
  • ●Improper adapter ligation

Compare With Similar Tests

TestDNA Library Preparation-ONT
ComparisonDNA Library Preparation-ONT

Frequently Asked Questions

What is DNA library preparation?
DNA library preparation is a laboratory process that prepares DNA samples for sequencing by adding adapters and other modifications, enabling the DNA to be read by sequencing platforms like Oxford Nanopore Technology.
Why is DNA library preparation needed for ONT sequencing?
ONT sequencing requires DNA libraries with specific adapters that facilitate the threading of DNA through nanopores. Proper library preparation ensures efficient sequencing and accurate data generation.
What is the cost of DNA Library Preparation-ONT at DNA Labs India?
The cost is INR 30000, which includes library preparation, sequencing, bioinformatics analysis, and a detailed report.
What sample type is required for this test?
The test requires extracted DNA. If you do not have extracted DNA, we can collect a blood or saliva sample for DNA extraction at an additional cost.
How long does the test take?
The turnaround time is 12 days from the time the sample reaches our laboratory.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across major cities in India.
What conditions can be diagnosed with this test?
This test is used for diagnosing genetic disorders such as developmental delays, intellectual disabilities, autism, certain cancers, cardiovascular diseases, and neurological disorders.
Is fasting required before the test?
No, fasting is not required for this test.
Will I receive a report?
Yes, you will receive a comprehensive report including genetic findings and interpretation.
Are there any risks associated with this test?
There are no physical risks. However, genetic results may have psychological implications, and we recommend genetic counseling.
Can this test be done during pregnancy?
Yes, but it is typically used for prenatal diagnosis only after consultation with a genetic specialist.
How accurate is ONT sequencing?
ONT sequencing provides long reads and is highly accurate for detecting structural variants. However, for single nucleotide variants, it may require higher coverage or complementary methods.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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