DNA QC and Quantitation - Nanodrop Test
Short Name: DNA QC Nanodrop
Also known as: Nanodrop DNA Quantitation, DNA Purity Check, Spectrophotometric DNA QC
DNA QC and Quantitation - Nanodrop Test test available at DNA Labs India for ₹200. Uses Spectrophotometry (Nanodrop) on Extracted DNA samples. Results in Reports are typically available within 4 days after sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SHAILAJA RAGHUNATH MURDESHWAR
Consultant Physician · Reg: 8052
Last reviewed: September 7, 2026
Overview
The primary purpose of DNA QC and Quantitation by Nanodrop is to assess the concentration and purity of extracted DNA samples. This ensures that the DNA is suitable for downstream applications, preventing wastage of time and resources on failed experiments. It also helps in standardizing input DNA amounts for assays like PCR and sequencing, thereby improving reproducibility and accuracy of results.
- Test Code
- 6332
- CPT Code
- 83890
- ICD Code
- Z01.89
- Price
- ₹200
- Sample Type
- Extracted DNA
- Result Time
- Reports are typically available within 4 days after sample receipt.
- Fasting Required
- No
- Method
- Spectrophotometry (Nanodrop)
Sample Collection
No special preparation is required. Ensure the DNA sample is already extracted and stored appropriately (e.g., in TE buffer or nuclease-free water).
Method: Laboratory collection or home collection
Laboratory Analysis
A small volume (1-2 µL) of the extracted DNA sample is placed on the Nanodrop pedestal. The measurement is taken within seconds.
Report Delivery
The sample is typically consumed during measurement; if more DNA is needed, ensure sufficient quantity is provided. Results are reported within 4 days.
Timeline: Reports are typically available within 4 days after sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of DNA QC and Quantitation by Nanodrop is to assess the concentration and purity of extracted DNA samples. This ensures that the DNA is suitable for downstream applications, preventing wastage of time and resources on failed experiments. It also helps in standardizing input DNA amounts for assays like PCR and sequencing, thereby improving reproducibility and accuracy of results.
How to Prepare
- Provide the extracted DNA sample in a sterile, labeled microcentrifuge tube.
- Ensure the sample volume is at least 5 µL to allow for measurement and repeat if needed.
- Store the sample at 2-8°C if shipping, or at -20°C for long-term storage before testing.
- Avoid repeated freeze-thaw cycles to maintain DNA integrity.
Doctor's Notes
Reviewed by Dr SHAILAJA RAGHUNATH MURDESHWAR — MBBS, MD (General Medicine) · Reg. No. 8052
"DNA quality is the cornerstone of reliable molecular testing. This Nanodrop-based QC ensures your samples meet stringent purity and concentration standards, minimizing the risk of failed experiments and erroneous results."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample not labeled or mislabeled
- Sample volume less than 2 µL
- Sample visibly degraded or contaminated
- Sample not in appropriate buffer (e.g., containing high salt or phenol)
- Sample received after prolonged storage at room temperature
Understanding Your Results
A260/A280 < 1.8
Protein contamination
Recommendation: Repurify the DNA using phenol-chloroform extraction or a commercial cleanup kit
A260/A280 > 2.0
RNA contamination
Recommendation: Treat with RNase and repurify
A260/A230 < 2.0
Salt or organic solvent contamination
Recommendation: Precipitate DNA with ethanol and wash with 70% ethanol
Concentration below expected
Low yield or degradation
Recommendation: Re-extract DNA or concentrate the sample using a vacuum concentrator
If you are a patient undergoing genetic testing, your physician will interpret the DNA QC results in the context of your clinical condition. If you are a researcher, consult your lab supervisor or molecular biology expert if results indicate poor DNA quality.
Limitations
- ⚠Cannot distinguish between DNA and RNA if both are present
- ⚠Not sensitive for low-concentration samples (<10 ng/µL)
- ⚠Does not assess DNA integrity (fragmentation)
- ⚠Contaminants absorbing at 260 nm can overestimate DNA concentration
- ⚠Requires complementary methods (e.g., gel electrophoresis) for comprehensive QC
Risks & Considerations
- ●No significant risks are associated with this test as it is performed on an extracted DNA sample. If a blood or tissue sample is being collected for DNA extraction, standard risks of venipuncture or biopsy apply.
Interfering Factors
- ●Presence of proteins, phenol, or other organic solvents
- ●RNA contamination
- ●Residual salts or chaotropic agents from extraction kits
- ●Incorrect blanking or calibration of the spectrophotometer
- ●Air bubbles or particulates in the sample
- ●Low DNA concentration (<10 ng/µL) leading to inaccurate readings
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Frequently Asked Questions
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