DNA QC and Quantitation - Qubit Test
Short Name: DNA QC Qubit
Also known as: Qubit DNA Quantitation, DNA Quality Control, DNA Concentration Measurement
DNA QC and Quantitation - Qubit Test test available at DNA Labs India for ₹400. Uses Fluorometry (Qubit), dsDNA BR assay on Extracted DNA samples. Results in Reports are typically available within 4 days after sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The primary purpose of DNA QC and quantitation is to assess the quality and quantity of DNA in a sample. This is critical because degraded, contaminated, or insufficient DNA can lead to failed experiments or inaccurate test results. By measuring DNA concentration with the Qubit fluorometer, we ensure that the sample meets the required standards for downstream applications. This test is often performed before genetic testing, such as paternity testing, carrier screening, or pharmacogenetic testing, to confirm that the DNA is suitable for analysis. It also helps in optimizing the amount of DNA used in PCR or sequencing reactions, preventing wastage of precious samples and reagents.
- Test Code
- 6335
- CPT Code
- 83885
- ICD Code
- Z01.89
- Price
- ₹400
- Sample Type
- Extracted DNA
- Result Time
- Reports are typically available within 4 days after sample receipt.
- Fasting Required
- No
- Method
- Fluorometry (Qubit), dsDNA BR assay
Sample Collection
No special preparation is required. If you are providing an extracted DNA sample, ensure it is stored appropriately (e.g., at -20°C) and transported on ice to maintain integrity.
Method: Provided by patient or referring lab; if home collection, DNA extraction is not included
Laboratory Analysis
The sample (extracted DNA) will be collected or received at the lab. If home collection is opted, a trained phlebotomist will visit your location to collect the sample (if applicable).
Report Delivery
The sample will be processed in the laboratory. Results will be available within 4 days and can be accessed online or via email/WhatsApp.
Timeline: Reports are typically available within 4 days after sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of DNA QC and quantitation is to assess the quality and quantity of DNA in a sample. This is critical because degraded, contaminated, or insufficient DNA can lead to failed experiments or inaccurate test results. By measuring DNA concentration with the Qubit fluorometer, we ensure that the sample meets the required standards for downstream applications. This test is often performed before genetic testing, such as paternity testing, carrier screening, or pharmacogenetic testing, to confirm that the DNA is suitable for analysis. It also helps in optimizing the amount of DNA used in PCR or sequencing reactions, preventing wastage of precious samples and reagents.
How to Prepare
- Provide the extracted DNA sample in a sterile, labeled microcentrifuge tube.
- Ensure the tube is clearly labeled with patient name and ID.
- Transport the sample on ice or at 2-8°C if delivery is delayed.
- Avoid repeated freeze-thaw cycles of the DNA sample.
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"DNA quality and quantity are critical for downstream applications like PCR, sequencing, and genotyping. Qubit fluorometry provides sensitive and accurate measurements, ensuring reliable results."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample not labeled or mislabeled
- Sample tube broken or leaking
- Insufficient volume (less than 1 µL)
- Sample heavily degraded or contaminated (if visible)
- Sample not stored properly (e.g., left at room temperature for extended periods)
Understanding Your Results
Sample is suitable for downstream applications such as PCR, sequencing, or genotyping.
Sample may be insufficient for certain tests; concentration may be needed or the test may need to be repeated with a larger sample.
DNA is fragmented; may not be suitable for tests requiring high molecular weight DNA, such as long-range PCR or certain sequencing platforms.
Presence of proteins or other contaminants; may require purification before proceeding.
If you are undergoing genetic testing and your DNA sample fails QC, your healthcare provider will guide you on the next steps, which may include providing a new sample or adjusting the testing approach. Consult your physician if you have concerns about the quality of your DNA sample or the results of this test.
Limitations
- ⚠Qubit measures only double-stranded DNA (dsDNA) if using the dsDNA BR assay; single-stranded DNA or RNA will not be detected accurately.
- ⚠The test does not assess DNA sequence integrity or functionality.
- ⚠Results are dependent on the quality of the extracted DNA provided; if the sample is heavily degraded, quantitation may be inaccurate.
- ⚠This test is not a diagnostic test for any disease; it is a preparatory step for other tests.
Risks & Considerations
- ●No significant risks are associated with this test as it is performed on an extracted DNA sample.
- ●If home collection is opted, there is a minimal risk of bruising or infection at the blood draw site (if blood is drawn for DNA extraction, but this test uses extracted DNA).
Interfering Factors
- ●Presence of RNA in the sample (if not treated with RNase)
- ●Contaminants like phenol, ethanol, or salts
- ●DNA degradation due to improper storage or handling
- ●Insufficient sample volume for accurate measurement
- ●Use of incorrect assay kit for the DNA type (e.g., ssDNA vs dsDNA)
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Frequently Asked Questions
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