Double Marker Test
Also known as: Maternal Serum Screening, First Trimester Screening, Dual Marker Test
Double Marker Test test available at DNA Labs India for ₹3,000. Uses Chemiluminescent Immunoassay on Serum samples. Results in Reports are usually delivered within 2-3 days after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The primary purpose of the Double Marker test is to screen for the risk of chromosomal abnormalities in the fetus. It is not a diagnostic test but a screening tool that helps identify pregnancies at higher risk. The test measures the levels of free beta-hCG and PAPP-A, which are produced by the placenta. Abnormal levels may indicate an increased risk of conditions such as Down syndrome (trisomy 21), Edwards syndrome (trisomy 18), or other chromosomal anomalies. Early identification of these risks enables healthcare providers to offer further diagnostic testing, such as amniocentesis or chorionic villus sampling (CVS), and to provide appropriate counseling and management. The test is particularly important for women over 35 years, as the risk of chromosomal abnormalities increases with maternal age. It is also recommended for women with a family history of genetic disorders, previous pregnancies with chromosomal abnormalities, or abnormal ultrasound findings.
- Test Code
- 6091
- CPT Code
- 84163
- ICD Code
- Z36.89
- Price
- ₹3,000
- Sample Type
- Serum
- Result Time
- Reports are usually delivered within 2-3 days after sample collection.
- Fasting Required
- No
- Method
- Chemiluminescent Immunoassay
Sample Collection
No special preparation is required. However, it is recommended to carry a doctor's prescription. Inform your healthcare provider about any medications or supplements you are taking.
Method: Venipuncture
Laboratory Analysis
A blood sample will be drawn from a vein in your arm. The procedure is quick and causes minimal discomfort.
Report Delivery
You can resume your normal activities immediately. There are no restrictions after the test.
Timeline: Reports are usually delivered within 2-3 days after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the Double Marker test is to screen for the risk of chromosomal abnormalities in the fetus. It is not a diagnostic test but a screening tool that helps identify pregnancies at higher risk. The test measures the levels of free beta-hCG and PAPP-A, which are produced by the placenta. Abnormal levels may indicate an increased risk of conditions such as Down syndrome (trisomy 21), Edwards syndrome (trisomy 18), or other chromosomal anomalies. Early identification of these risks enables healthcare providers to offer further diagnostic testing, such as amniocentesis or chorionic villus sampling (CVS), and to provide appropriate counseling and management. The test is particularly important for women over 35 years, as the risk of chromosomal abnormalities increases with maternal age. It is also recommended for women with a family history of genetic disorders, previous pregnancies with chromosomal abnormalities, or abnormal ultrasound findings.
How to Prepare
- No fasting required
- Carry a valid doctor's prescription
- Inform the lab if you are on any medications
- Ensure the test is done between 11 and 13 weeks of pregnancy
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"The Double Marker test is a crucial first-trimester screening tool. Early detection of chromosomal abnormalities allows for informed decision-making and timely management. I recommend it for all pregnant women, especially those above 35 years or with a family history of genetic disorders."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed samples
- Lipemic samples
- Incorrect labeling
- Sample collected after 13 weeks of gestation
Understanding Your Results
Both markers within normal range (0.5-2.0 MoM)
Lower probability of chromosomal abnormalities; routine prenatal care continues.
Elevated free beta-hCG and low PAPP-A
Increased risk of trisomy 21; further diagnostic testing recommended.
Low free beta-hCG and low PAPP-A
Increased risk of trisomy 18; further diagnostic testing recommended.
Consult your doctor if you receive an abnormal result or if you have any concerns about your pregnancy. Your doctor will discuss the next steps, which may include diagnostic tests like amniocentesis or CVS, and provide appropriate counseling.
Limitations
- ⚠Screening test, not diagnostic; false positives and false negatives possible
- ⚠Results are risk estimates, not definitive diagnoses
- ⚠Abnormal results require confirmatory diagnostic testing (amniocentesis, CVS)
- ⚠Not recommended after 13 weeks of gestation
Risks & Considerations
- ●Minimal risk of bruising or infection at the needle site
- ●No risk to the fetus as the test is non-invasive
Interfering Factors
- ●Gestational age at the time of testing (must be between 11 and 13 weeks)
- ●Multiple pregnancies (twins, triplets) can affect hormone levels
- ●Maternal weight and ethnicity
- ●Smoking during pregnancy
- ●In vitro fertilization (IVF) pregnancies
Compare With Similar Tests
| Test | Double Marker | Quadruple Marker Test | NIPT (Non-Invasive Prenatal Testing) | Amniocentesis |
|---|---|---|---|---|
| Comparison | Double Marker | Quadruple marker test is performed in the second trimester (15-20 weeks) and measures four markers (AFP, hCG, uE3, inhibin-A). It has a higher detection rate for Down syndrome compared to the double marker test. | NIPT analyzes fetal DNA in maternal blood and has a higher accuracy (99%) for detecting trisomies. It is more expensive but can be done from 10 weeks onward. | Amniocentesis is a diagnostic test that involves sampling amniotic fluid. It is invasive and carries a small risk of miscarriage but provides a definitive diagnosis. |
Frequently Asked Questions
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