Eukaryotic mRNA Sequencing and De Novo Analysis Test
Short Name: mRNA Seq De Novo
Also known as: mRNA Sequencing, Transcriptome Sequencing, De Novo Transcriptome Analysis
Eukaryotic mRNA Sequencing and De Novo Analysis Test test available at DNA Labs India for ₹35,000. Uses Next Generation Sequencing (NGS), Bioinformatics Analysis on Extracted DNA samples. Results in Results are typically available within 8 weeks after the sample reaches the laboratory.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The primary purpose of eukaryotic mRNA sequencing and de novo analysis is to provide a comprehensive view of gene expression and identify genetic abnormalities that may be responsible for various diseases. This test is used to: 1) Detect novel or known mutations in genes associated with rare genetic disorders. 2) Identify alternative splicing events that may lead to dysfunctional proteins. 3) Quantify gene expression levels to understand disease mechanisms. 4) Discover novel transcripts and non-coding RNAs that may play a role in disease. 5) Aid in cancer diagnosis by identifying mutations in oncogenes and tumor suppressor genes. 6) Guide targeted therapy selection based on the specific genetic profile of a tumor. 7) Support research in fields such as developmental biology and pharmacogenomics.
- Test Code
- 6427
- CPT Code
- 81450
- ICD Code
- Z01.89
- Price
- ₹35,000
- Sample Type
- Extracted DNA
- Result Time
- Results are typically available within 8 weeks after the sample reaches the laboratory.
- Fasting Required
- No
- Method
- Next Generation Sequencing (NGS), Bioinformatics Analysis
Sample Collection
No special preparation is required. However, inform your healthcare provider about any medications you are taking, as some drugs may affect gene expression.
Method: Blood sample or tissue biopsy
Laboratory Analysis
A blood sample will be drawn from a vein in your arm, or a tissue biopsy may be performed if required. The procedure is routine and minimally invasive.
Report Delivery
You can resume normal activities immediately. There are no restrictions post-sample collection.
Timeline: Results are typically available within 8 weeks after the sample reaches the laboratory.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of eukaryotic mRNA sequencing and de novo analysis is to provide a comprehensive view of gene expression and identify genetic abnormalities that may be responsible for various diseases. This test is used to: 1) Detect novel or known mutations in genes associated with rare genetic disorders. 2) Identify alternative splicing events that may lead to dysfunctional proteins. 3) Quantify gene expression levels to understand disease mechanisms. 4) Discover novel transcripts and non-coding RNAs that may play a role in disease. 5) Aid in cancer diagnosis by identifying mutations in oncogenes and tumor suppressor genes. 6) Guide targeted therapy selection based on the specific genetic profile of a tumor. 7) Support research in fields such as developmental biology and pharmacogenomics.
How to Prepare
- Ensure the sample is collected in a sterile container
- For blood samples, use EDTA or PAXgene tubes
- For tissue samples, ensure proper preservation in RNA later or snap-freeze
- Label the sample with patient ID and date
- Transport the sample to the lab on dry ice if possible
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"This test is particularly valuable when standard genetic testing fails to identify a causative variant. It provides a comprehensive view of gene expression and can reveal splice variants and novel transcripts that may be missed by DNA-based tests."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed blood samples
- Samples with visible microbial contamination
- Insufficient RNA quantity (< 100 ng)
- RNA integrity number (RIN) < 7
- Improperly labeled samples
Understanding Your Results
Upregulation of oncogenes (e.g., MYC, KRAS)
May indicate active cancer or increased cancer risk
Downregulation of tumor suppressor genes (e.g., TP53)
May contribute to tumorigenesis
Novel splice variants in disease-associated genes
May disrupt protein function and cause genetic disorders
High expression of specific immune checkpoint genes
May guide immunotherapy decisions
No significant abnormalities
May indicate no transcriptomic cause for symptoms
Consult your referring physician or a genetic counselor to discuss the results and their implications for your health. If the test identifies a pathogenic variant, you may be referred to a specialist for further management.
Limitations
- ⚠De novo assembly may not capture all transcripts, especially low-abundance ones
- ⚠Requires high-quality RNA; degraded samples may yield incomplete results
- ⚠Interpretation of variants requires expert analysis and may not always be clinically actionable
- ⚠Not a substitute for whole genome sequencing in all cases
- ⚠Cost may be prohibitive for some patients
Risks & Considerations
- ●Minimal risk of bleeding or bruising at the needle site
- ●Rare risk of infection
- ●No radiation exposure
Interfering Factors
- ●RNA degradation due to improper sample handling
- ●Contamination with genomic DNA
- ●Low RNA quality or quantity
- ●Presence of highly abundant transcripts masking low-expression genes
- ●Bioinformatics pipeline variations
Compare With Similar Tests
| Test | Eukaryotic mRNA Sequencing and De Novo Analysis | Whole Exome Sequencing | Whole Genome Sequencing | Targeted Gene Panels |
|---|---|---|---|---|
| Comparison | Eukaryotic mRNA Sequencing and De Novo Analysis |
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