Eukaryotic mRNA Sequencing and Reference Based Analysis-Low Input Test
Short Name: mRNA Sequencing
Also known as: mRNA Seq, Transcriptome Sequencing, RNA Sequencing
Eukaryotic mRNA Sequencing and Reference Based Analysis-Low Input Test test available at DNA Labs India for ₹26,000. Uses Next-Generation Sequencing (NGS), Bioinformatics Analysis on Extracted DNA samples. Results in Results are typically available within 8 weeks due to the complex bioinformatics analysis involved.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The primary purpose of this test is to identify genetic variations and expression abnormalities that cause disease. It is used when standard diagnostic tests are inconclusive, or when a condition is suspected to have a genetic basis. The test helps in confirming diagnoses, predicting disease progression, and selecting targeted therapies.
- Test Code
- 6425
- CPT Code
- 81445
- ICD Code
- Z01.89
- Price
- ₹26,000
- Sample Type
- Extracted DNA
- Result Time
- Results are typically available within 8 weeks due to the complex bioinformatics analysis involved.
- Fasting Required
- No
- Method
- Next-Generation Sequencing (NGS), Bioinformatics Analysis
Sample Collection
No special preparation required. Inform your doctor about any medications you are taking.
Method: Blood draw or tissue biopsy
Laboratory Analysis
A blood sample will be drawn from a vein in your arm. If a tissue biopsy is needed, it will be performed by a specialist.
Report Delivery
You can resume normal activities immediately. There may be minor bruising at the puncture site, which is normal.
Timeline: Results are typically available within 8 weeks due to the complex bioinformatics analysis involved.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of this test is to identify genetic variations and expression abnormalities that cause disease. It is used when standard diagnostic tests are inconclusive, or when a condition is suspected to have a genetic basis. The test helps in confirming diagnoses, predicting disease progression, and selecting targeted therapies.
How to Prepare
- Ensure the sample is collected in an EDTA tube
- Label the tube with patient details
- Transport the sample to the lab within 24 hours
- Avoid freezing the sample
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"This test is particularly valuable for patients with suspected genetic disorders where conventional testing has been inconclusive. It provides a comprehensive view of gene expression, aiding in precise diagnosis and targeted therapy."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed sample
- Clotted sample
- Insufficient quantity
- Improper labeling
- Sample received after 24 hours at room temperature
Understanding Your Results
Pathogenic variant detected
Confirms genetic cause; may guide targeted therapy
Variant of uncertain significance (VUS)
Further testing or family studies may be needed
No pathogenic variants
Genetic cause less likely; other tests may be considered
If you have received this test, it is likely because your doctor suspects a genetic condition. After receiving results, schedule a follow-up with your physician to discuss implications and next steps.
Limitations
- ⚠Detects only mRNA, not non-coding RNA
- ⚠May miss large structural variants
- ⚠Requires high-quality RNA
- ⚠Interpretation may be limited by reference genome completeness
- ⚠Not a substitute for whole genome sequencing
Risks & Considerations
- ●Minor bleeding or bruising at the puncture site
- ●Infection (rare)
- ●Emotional distress from results
Interfering Factors
- ●Poor RNA quality or degradation
- ●Contamination with genomic DNA
- ●Low input RNA quantity
- ●Presence of haemoglobin in blood samples
- ●Recent blood transfusion
- ●Medications affecting gene expression
Compare With Similar Tests
| Test | Eukaryotic mRNA Sequencing and Reference Based Analysis-Low Input | Whole Exome Sequencing | Targeted Gene Panel | Microarray |
|---|---|---|---|---|
| Comparison | Eukaryotic mRNA Sequencing and Reference Based Analysis-Low Input | WES sequences all coding regions, while mRNA sequencing focuses on expressed genes. mRNA seq can detect expression changes but may miss non-expressed variants. | Targeted panels analyze specific genes, whereas mRNA seq provides a broader transcriptome view. mRNA seq is more comprehensive but may be more expensive. | Microarray detects copy number variations, while mRNA seq detects expression and sequence changes. mRNA seq offers higher resolution for expression analysis. |
Frequently Asked Questions
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Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
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