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DNA Labs India

Eukaryotic mRNA Sequencing and Reference Based Analysis-Ultra Low Input Test

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Eukaryotic mRNA Sequencing and Reference Based Analysis-Ultra Low Input Test

Short Name: mRNA Seq (Ultra Low Input)

Also known as: mRNA Sequencing, Transcriptome Sequencing, RNA-Seq (Ultra Low Input)

Eukaryotic mRNA Sequencing and Reference Based Analysis-Ultra Low Input Test test available at DNA Labs India for ₹28,000. Uses Next-Generation Sequencing (NGS), Reference-based transcriptome analysis on Extracted DNA samples. Results in Reports are typically available within 8 weeks from sample receipt.. Free home collection in 300+ cities across India.

Molecular🏠 Home Collection

🩺 Medically Reviewed By

Overview

The purpose of this test is to analyze the expression levels of thousands of genes simultaneously. By comparing the mRNA sequences to a reference genome, we can identify differentially expressed genes, which may indicate disease states, drug responses, or developmental stages. This information aids in diagnosing genetic conditions, classifying tumors, and guiding treatment decisions.

Test Code
6424
CPT Code
81450
ICD Code
Z01.89
Price
₹28,000
Sample Type
Extracted DNA
Result Time
Reports are typically available within 8 weeks from sample receipt.
Fasting Required
No
Method
Next-Generation Sequencing (NGS), Reference-based transcriptome analysis
Step 1

Sample Collection

No special preparation required. Inform your doctor about any medications or supplements.

Method: Blood or tissue sample

Step 2

Laboratory Analysis

A blood sample will be drawn by a trained phlebotomist. For tissue samples, a biopsy procedure will be performed.

Step 3

Report Delivery

You can resume normal activities immediately. The sample will be processed in the laboratory.

Timeline: Reports are typically available within 8 weeks from sample receipt.

Patient Instructions

1
Before the Test:No special preparation required. Ensure you have a valid prescription from a healthcare provider.
2
During the Test:A blood sample will be collected. For tissue samples, a biopsy may be performed.
3
After the Test:You can resume normal activities. The sample will be sent to the lab for analysis.

About This Test

Who Should Get This Test

The purpose of this test is to analyze the expression levels of thousands of genes simultaneously. By comparing the mRNA sequences to a reference genome, we can identify differentially expressed genes, which may indicate disease states, drug responses, or developmental stages. This information aids in diagnosing genetic conditions, classifying tumors, and guiding treatment decisions.

How to Prepare

  • Use EDTA or citrate tube for blood collection
  • For tissue, ensure proper preservation in RNA later or snap-freeze
  • Label the sample with patient ID and date
  • Transport on dry ice if possible

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"This test provides a comprehensive view of gene expression, aiding in the diagnosis of genetic disorders and guiding targeted therapies."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeExtracted DNA
Sample Volume10-100 ng
ContainerEppendorf tube (DNA)
Collection MethodBlood or tissue sample

Sample Stability

Blood: 24 hours at 2-8°C
Extracted DNA: 1 week at -20°C
RNA: 1 month at -80°C
Sample Rejection Criteria:
  • Hemolyzed blood samples
  • Insufficient quantity of DNA/RNA
  • Degraded RNA (RIN < 7)
  • Improperly labeled samples

Understanding Your Results

The results of this test are interpreted by clinical geneticists and bioinformaticians. A detailed report will include gene expression levels, significant differentially expressed genes, and any relevant mutations or fusions.
High expression of oncogenes may suggest malignancy
Loss of expression of tumor suppressor genes may indicate cancer
Specific gene fusions (e.g., BCR-ABL) are diagnostic for certain leukemias
Splice variants may affect protein function and disease phenotype
⚠️ When to Consult a Doctor:

Consult your referring physician or a genetic counselor to discuss the results and their implications for your health or treatment plan.

Limitations

  • Only detects expressed genes; mutations in non-expressed regions may be missed
  • Reference-based analysis requires a high-quality reference genome
  • May not detect low-abundance transcripts without deep sequencing
  • Interpretation requires expertise in genomics

Risks & Considerations

  • Minimal risk of bleeding or infection at the blood draw site
  • For biopsy, there is a small risk of bleeding or infection

Interfering Factors

  • RNA degradation due to improper sample handling
  • Contamination with genomic DNA
  • Low RNA quality or quantity
  • Batch effects in sequencing
  • Bioinformatics pipeline variations

Compare With Similar Tests

TestEukaryotic mRNA Sequencing and Reference Based Analysis-Ultra Low InputRT-PCRMicroarrayWhole Exome Sequencing
ComparisonEukaryotic mRNA Sequencing and Reference Based Analysis-Ultra Low Input

Frequently Asked Questions

What is the cost of the Eukaryotic mRNA Sequencing test?
The cost is INR 28000, which includes home sample collection and comprehensive analysis.
What sample is required for this test?
Extracted DNA is required. The sample can be obtained from blood or tissue.
How long does it take to get results?
Reports are typically available within 8 weeks.
Is fasting required before the test?
No, fasting is not required.
Can this test detect cancer?
It can help in cancer diagnosis by identifying gene expression changes and mutations.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings.
What is reference-based analysis?
It involves comparing mRNA sequences to a reference genome to identify gene expression levels and variations.
What is the difference between mRNA sequencing and DNA sequencing?
mRNA sequencing analyzes gene expression, while DNA sequencing looks at the genetic code itself.
Can this test be used for research purposes?
Yes, it is widely used in research for transcriptome profiling.
Are there any risks associated with the test?
The test is safe with minimal risks like any blood draw.
Will insurance cover the cost?
Insurance coverage varies; please check with your provider.
How should I prepare for the test?
No special preparation is needed. Just follow your doctor's instructions.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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