Eukaryotic Stranded mRNA Sequencing and Reference Based Analysis Test
Short Name: mRNA Sequencing
Also known as: Stranded mRNA Seq, Transcriptome Sequencing, RNA-Seq
Eukaryotic Stranded mRNA Sequencing and Reference Based Analysis Test test available at DNA Labs India for ₹24,000. Uses Next-Generation Sequencing (NGS), Stranded mRNA library preparation, Reference-based alignment on Extracted DNA samples. Results in Results are typically delivered within 8 weeks after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The purpose of this test is to analyze the gene expression profile of eukaryotic cells. It helps in identifying differentially expressed genes, detecting disease-related mutations, understanding gene regulation, and aiding in the development of targeted therapies. It is also used in oncology to classify tumors, in rare disease diagnosis, and in pharmacogenomics to predict drug response.
- Test Code
- 6420
- CPT Code
- 81450
- ICD Code
- Z01.89
- Price
- ₹24,000
- Sample Type
- Extracted DNA
- Result Time
- Results are typically delivered within 8 weeks after sample collection.
- Fasting Required
- No
- Method
- Next-Generation Sequencing (NGS), Stranded mRNA library preparation, Reference-based alignment
Sample Collection
No special preparation required. Inform your doctor about any medications or supplements you are taking.
Method: Blood or tissue biopsy
Laboratory Analysis
A blood sample will be drawn from a vein in your arm. For tissue biopsy, a small sample will be collected by a healthcare professional.
Report Delivery
You can resume normal activities immediately. The sample will be sent to the laboratory for analysis.
Timeline: Results are typically delivered within 8 weeks after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of this test is to analyze the gene expression profile of eukaryotic cells. It helps in identifying differentially expressed genes, detecting disease-related mutations, understanding gene regulation, and aiding in the development of targeted therapies. It is also used in oncology to classify tumors, in rare disease diagnosis, and in pharmacogenomics to predict drug response.
How to Prepare
- Ensure the sample is collected in a sterile container
- For blood, use EDTA tube to prevent clotting
- Label the sample with patient ID and date
- Transport the sample on dry ice if possible
- Avoid repeated freeze-thaw cycles
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"This test provides critical insights into gene expression patterns, aiding in the diagnosis and management of genetic disorders and cancers."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed blood sample
- Insufficient quantity
- Improper labeling
- Sample not stored at correct temperature
- RNA degraded (RIN < 7)
Understanding Your Results
Normal expression profile
No significant gene expression abnormalities detected.
Overexpression of oncogene
May indicate cancer or proliferative disorder.
Underexpression of tumor suppressor
Could contribute to tumorigenesis.
Gene fusion detected
May be a driver mutation in cancer; targeted therapy may be considered.
Splice variant alteration
May affect protein function; further validation required.
Consult your physician if you have symptoms suggestive of a genetic disorder, if you have a family history of cancer, or if you are considering personalized treatment options.
Limitations
- ⚠Only detects expressed genes; non-coding RNAs not analyzed
- ⚠Reference genome may not cover all genetic variations
- ⚠Low expression genes may not be detected
- ⚠Requires high-quality RNA; degraded samples may fail
- ⚠Bioinformatics analysis may miss rare splice variants
Risks & Considerations
- ●Minor bleeding or bruising at the blood draw site
- ●Infection at biopsy site (rare)
- ●Emotional distress from unexpected findings
Interfering Factors
- ●RNA degradation due to improper sample handling
- ●Contamination with genomic DNA
- ●Low RNA quality or quantity
- ●Presence of hemoglobin in blood samples
- ●Incorrect storage temperature
Compare With Similar Tests
| Test | Eukaryotic Stranded mRNA Sequencing and Reference Based Analysis | Whole Exome Sequencing | Targeted Gene Panel | Microarray |
|---|---|---|---|---|
| Comparison | Eukaryotic Stranded mRNA Sequencing and Reference Based Analysis |
Frequently Asked Questions
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Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
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