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Eukaryotic Stranded Transcriptome Library Preparation- Including lncRNA Test

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Eukaryotic Stranded Transcriptome Library Preparation- Including lncRNA Test

Short Name: Stranded Transcriptome Library Prep

Also known as: Stranded RNA-Seq Library Prep, lncRNA Library Preparation, Eukaryotic Transcriptome Sequencing

Eukaryotic Stranded Transcriptome Library Preparation- Including lncRNA Test test available at DNA Labs India for ₹16,500. Uses cDNA Synthesis, Stranded Library Preparation, Next-Generation Sequencing on Extracted RNA samples. Results in 1 week. Free home collection in 300+ cities across India.

Library Preparation🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SULOCHANA HEMCHANDRA HOLLA

Dr SULOCHANA HEMCHANDRA HOLLA

Consultant Medical Geneticist · Reg: 8532

Last reviewed: September 7, 2026

Overview

To prepare a strand-specific cDNA library from eukaryotic RNA for sequencing, enabling detailed analysis of gene expression, identification of non-coding RNAs like lncRNAs, and research into transcriptomic regulation.

Test Code
3432
Price
₹16,500
Sample Type
Extracted RNA
Result Time
1 week
Fasting Required
No
Method
cDNA Synthesis, Stranded Library Preparation, Next-Generation Sequencing
Step 1

Sample Collection

Ensure RNA is extracted using RNase-free methods and meets quality standards (e.g., RIN >7).

Method: Laboratory extraction

Step 2

Laboratory Analysis

Handle samples with care to avoid degradation; use appropriate lab safety protocols.

Step 3

Report Delivery

Store extracted RNA at -80°C until shipment; ship on dry ice to maintain integrity.

Timeline: 1 week

Patient Instructions

1
Before the Test:Verify RNA quality and quantity; ensure samples are properly extracted and stored.
2
During the Test:Library preparation and sequencing are performed in a controlled laboratory environment following standardized protocols.
3
After the Test:Data analysis is conducted, and a report is generated with expression profiles and identified RNAs.

About This Test

Who Should Get This Test

To prepare a strand-specific cDNA library from eukaryotic RNA for sequencing, enabling detailed analysis of gene expression, identification of non-coding RNAs like lncRNAs, and research into transcriptomic regulation.

How to Prepare

  • Use RNase-free equipment during extraction
  • Avoid freeze-thaw cycles
  • Label samples clearly
  • Ship with cold packs or dry ice

Doctor's Notes

Reviewed by — MBBS, MD (Medical Genetics) · Reg. No. 8532

"This advanced library preparation technique is essential for accurate transcriptome analysis, aiding in the discovery of novel RNA biomarkers and understanding gene regulation."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeExtracted RNA
Sample VolumeAs required
ContainerRNase-free tube
Collection MethodLaboratory extraction

Sample Stability

RNA stable at -80°C for up to 6 months
Avoid repeated freezing and thawing
Sample Rejection Criteria:
  • Degraded RNA (RIN <5)
  • Contaminated samples
  • Insufficient volume or concentration
  • Improperly labeled samples

Understanding Your Results

Results provide detailed insights into gene expression patterns, strand-specific transcription, and identification of non-coding RNAs such as lncRNAs, which are crucial for understanding regulatory mechanisms.
High expression of lncRNAs may indicate roles in gene regulation or disease pathways
Compare expression levels with control samples or databases
Use bioinformatics tools for pathway analysis
Consult with a geneticist for complex datasets
⚠️ When to Consult a Doctor:

Consult a geneticist or research supervisor for detailed interpretation of results, especially for clinical or translational research applications.

Limitations

  • ⚠Requires high-quality RNA input
  • ⚠Not intended for diagnostic use
  • ⚠Results depend on sequencing depth and bioinformatics analysis
  • ⚠Limited to eukaryotic organisms

Risks & Considerations

  • ●No direct physical risks to participants, as this is a laboratory technique
  • ●Potential for data misinterpretation without expert guidance

Interfering Factors

  • ●RNA degradation
  • ●Contamination with DNA or RNases
  • ●Low RNA quality or quantity
  • ●Improper storage conditions

Compare With Similar Tests

TestEukaryotic Stranded Transcriptome Library Preparation- Including lncRNANon-Stranded Transcriptome Library PrepWhole Transcriptome SequencingmRNA SequencingSmall RNA Sequencing
ComparisonEukaryotic Stranded Transcriptome Library Preparation- Including lncRNA

Frequently Asked Questions

What is eukaryotic stranded transcriptome library preparation?
It is a method to convert RNA into a cDNA library for sequencing while preserving strand information, allowing identification of both coding and non-coding RNAs.
Why is strand separation important in RNA sequencing?
Strand separation helps distinguish between sense and antisense transcripts, improving accuracy and enabling detection of non-coding RNAs like lncRNAs.
What are lncRNAs and why are they important?
Long non-coding RNAs (lncRNAs) are RNA molecules that do not code for proteins but regulate gene expression and are implicated in various diseases.
How much does this test cost at DNA Labs India?
The cost is INR 16,500, which includes library preparation, sequencing, and basic data analysis.
What sample type is required for this test?
Extracted RNA of high quality (RIN >7) is required, typically from eukaryotic sources.
How long does it take to get the results?
Results are typically delivered within 1 week via online portal, email, or WhatsApp.
Is home sample collection available?
Yes, free home sample collection is available for online bookings across India.
What are the benefits of this test over non-stranded methods?
Benefits include improved accuracy, ability to identify non-coding RNAs, and a more comprehensive view of gene expression.
Can this test be used for diagnostic purposes?
No, this test is intended for research use only and not for clinical diagnosis.
What is the turnaround time for the report?
The turnaround time is 1 week from sample receipt.
How should I prepare the sample for this test?
Ensure RNA is extracted using RNase-free methods, stored at -80°C, and shipped on dry ice to prevent degradation.
Is this test covered by insurance?
Generally, this research test is not covered by insurance; check with your provider for specific funding options.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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