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Eukaryotic Stranded Transcriptome Sequencing and Reference Based Analysis-Including lncRNA Test

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Eukaryotic Stranded Transcriptome Sequencing and Reference Based Analysis-Including lncRNA Test

Short Name: Eukaryotic Stranded Transcriptome Sequencing

Also known as: Stranded RNA-Seq with lncRNA Analysis, Transcriptome Sequencing with Reference-Based Analysis

Eukaryotic Stranded Transcriptome Sequencing and Reference Based Analysis-Including lncRNA Test test available at DNA Labs India for ₹50,000. Uses Next-Generation Sequencing (NGS), Stranded RNA-Seq, Reference-based bioinformatics analysis on Extracted DNA samples. Results in Reports are delivered within 8 weeks from sample receipt.. Free home collection in 300+ cities across India.

Next-Generation Sequencing🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

The primary purpose of this test is to provide a comprehensive and quantitative profile of the transcriptome in a given sample. It is used to identify differentially expressed genes, characterize alternative splicing, detect fusion transcripts, and quantify lncRNA expression. This information is valuable for research applications, biomarker discovery, and understanding the molecular basis of diseases such as cancer, neurological disorders, and developmental abnormalities. The test can also reveal novel transcripts that may serve as potential therapeutic targets.

Test Code
6426
CPT Code
81450
ICD Code
Z01.89
Price
₹50,000
Sample Type
Extracted DNA
Result Time
Reports are delivered within 8 weeks from sample receipt.
Fasting Required
No
Method
Next-Generation Sequencing (NGS), Stranded RNA-Seq, Reference-based bioinformatics analysis
Step 1

Sample Collection

No special preparation is required. However, ensure that the sample is collected in an appropriate RNA-stabilizing tube if blood is used. For tissue samples, immediate freezing or preservation in RNA later is recommended.

Method: Blood draw or tissue biopsy

Step 2

Laboratory Analysis

Sample collection will be performed by a trained phlebotomist. For blood, a standard venipuncture will be done. For tissue, a biopsy procedure may be required.

Step 3

Report Delivery

The sample should be transported to the laboratory on dry ice or in a RNA-stable medium. Avoid repeated freeze-thaw cycles.

Timeline: Reports are delivered within 8 weeks from sample receipt.

Patient Instructions

1
Before the Test:No specific preparation required. Ensure you have a valid prescription or research protocol.
2
During the Test:The test involves sample collection and subsequent laboratory processing. No discomfort is expected.
3
After the Test:You will receive the report via email/portal. Discuss results with your healthcare provider or research team.

About This Test

Who Should Get This Test

The primary purpose of this test is to provide a comprehensive and quantitative profile of the transcriptome in a given sample. It is used to identify differentially expressed genes, characterize alternative splicing, detect fusion transcripts, and quantify lncRNA expression. This information is valuable for research applications, biomarker discovery, and understanding the molecular basis of diseases such as cancer, neurological disorders, and developmental abnormalities. The test can also reveal novel transcripts that may serve as potential therapeutic targets.

How to Prepare

  • Use RNA-free collection tubes
  • For blood, use PAXgene or Tempus tubes
  • For tissue, snap-freeze in liquid nitrogen or use RNA later
  • Label the sample with patient ID and date
  • Transport on dry ice

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"This test provides a comprehensive view of the transcriptome, enabling identification of differentially expressed genes and novel lncRNAs that may serve as biomarkers or therapeutic targets."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeExtracted DNA
Sample Volume1 µg
ContainerEppendorf tube
Collection MethodBlood draw or tissue biopsy

Sample Stability

Blood in PAXgene tube: 3 days at room temperature, 5 days at 4°C
Tissue in RNA later: 1 week at room temperature, 1 month at 4°C
Extracted RNA: 1 month at -80°C
Sample Rejection Criteria:
  • Hemolyzed blood samples
  • RNA degradation (RIN < 7)
  • Insufficient RNA quantity (< 100 ng)
  • Improper storage or transport conditions

Understanding Your Results

The results of this test are complex and require expert bioinformatics analysis. The report will include gene expression levels, differential expression analysis, splicing events, and lncRNA profiles. Interpretation should be done in the context of the research question or clinical scenario.
Gene expression levels are reported as FPKM or TPM values; higher values indicate higher expression.
Differential expression is shown as log2 fold change with statistical significance (p-value).
Alternative splicing events are quantified using PSI values; changes in PSI indicate altered splicing.
lncRNA expression is reported similarly to mRNA; some lncRNAs may be novel and require further validation.
Novel transcripts are flagged for further investigation.
⚠️ When to Consult a Doctor:

If you are considering this test for clinical purposes, consult a geneticist or oncologist to understand the implications. For research, consult your research coordinator.

Limitations

  • ⚠Reference-based analysis may miss transcripts not present in the reference genome
  • ⚠Lowly expressed genes may not be detected due to sequencing depth
  • ⚠Requires high-quality RNA; degraded samples may yield poor results
  • ⚠Interpretation of novel transcripts requires validation
  • ⚠Not a diagnostic test; results are for research purposes

Risks & Considerations

  • ●Minimal risk of bleeding or infection at blood draw site
  • ●No radiation exposure
  • ●Psychological impact of unexpected findings (if any)

Interfering Factors

  • ●RNA degradation due to improper sample handling
  • ●Contamination with genomic DNA
  • ●Low RNA quality or quantity
  • ●Batch effects in sequencing runs
  • ●Bioinformatics pipeline variations

Compare With Similar Tests

TestEukaryotic Stranded Transcriptome Sequencing and Reference Based Analysis-Including lncRNAWhole Transcriptome Sequencing (WTS)MicroarrayqRT-PCR
ComparisonEukaryotic Stranded Transcriptome Sequencing and Reference Based Analysis-Including lncRNA

Frequently Asked Questions

What is the difference between stranded and non-stranded RNA-seq?
Stranded RNA-seq preserves the orientation of RNA transcripts, allowing detection of antisense transcription and strand-specific expression. Non-stranded methods lose this information.
What is the turnaround time for this test?
The turnaround time is 8 weeks from sample receipt.
Is home sample collection available?
Yes, we offer free home sample collection for online bookings across major cities in India.
What type of sample is required?
The sample type is extracted DNA, but for RNA-seq, RNA is typically extracted from blood or tissue. Please confirm with our team.
Can this test detect lncRNAs?
Yes, the test includes analysis of long non-coding RNAs (lncRNAs).
Is this test diagnostic?
No, it is a research tool and not intended for diagnostic purposes.
What is the cost of the test?
The test costs INR 50,000.
How should the sample be transported?
Samples should be transported on dry ice or in RNA-stabilizing solution.
What is the minimum RNA quantity required?
A minimum of 100 ng of high-quality RNA (RIN > 7) is required.
Can this test be used for cancer research?
Yes, it is commonly used to study gene expression changes in cancer.
Are there any fasting requirements?
No, fasting is not required.
How will I receive my report?
Reports are delivered via online portal, email, or WhatsApp.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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