Hepatitis D Virus Detection PCR Test
Short Name: HDV Detection PCR Test
Also known as: HDV RNA PCR Test, Hepatitis D PCR Test, Hepatitis D Virus RNA Detection
Hepatitis D Virus Detection PCR Test test available at DNA Labs India for ₹8,500. Uses PCR on Plasma samples. Results in Results are typically available within 13 days after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
This test is designed to detect Hepatitis D Virus (HDV) RNA in blood samples, aiding in the diagnosis of HDV infection, monitoring viral load during treatment, and assessing the effectiveness of antiviral therapies. It is essential for early detection in at-risk populations, such as those with chronic Hepatitis B, to prevent severe liver complications.
- Test Code
- 728
- Price
- ₹8,500
- Sample Type
- Plasma
- Result Time
- Results are typically available within 13 days after sample collection.
- Fasting Required
- Yes (12 hours)
- Method
- PCR
Sample Collection
Patient should fast for 12 hours before sample collection. Inform the healthcare provider about any medications or recent infections.
Method: Venipuncture
Laboratory Analysis
A blood sample is drawn from a vein in the arm using standard venipuncture technique. The sample is collected in an EDTA tube.
Report Delivery
Apply pressure to the puncture site to prevent bleeding. Keep the bandage on for a few hours. Avoid strenuous activity with the arm used for collection.
Timeline: Results are typically available within 13 days after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
This test is designed to detect Hepatitis D Virus (HDV) RNA in blood samples, aiding in the diagnosis of HDV infection, monitoring viral load during treatment, and assessing the effectiveness of antiviral therapies. It is essential for early detection in at-risk populations, such as those with chronic Hepatitis B, to prevent severe liver complications.
How to Prepare
- Collect 4 mL (minimum 2 mL) of blood in a Lavender Top (EDTA) tube.
- Separate plasma aseptically within 2 hours of collection.
- Ship refrigerated (2-8°C) or frozen (-20°C or below) to maintain stability.
- Ensure proper labeling and documentation of the sample.
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Early detection of Hepatitis D through PCR is crucial for managing liver health, especially in patients with chronic Hepatitis B. Timely diagnosis can prevent severe complications like liver failure."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed, lipemic, or icteric samples.
- Samples not collected in EDTA tubes.
- Insufficient sample volume (<2 mL).
- Samples not separated or stored correctly within specified times.
- Improperly labeled or contaminated samples.
Understanding Your Results
Not Detected
No Hepatitis D Virus RNA found. Suggests absence of active HDV infection. If clinical suspicion remains, repeat testing or antibody tests may be considered.
Action: Consult a healthcare provider for further evaluation if symptoms persist.
Detected
Hepatitis D Virus RNA present, indicating active HDV infection. May require immediate medical attention and treatment planning.
Action: Consult an infectious disease specialist or hepatologist for management and monitoring.
If test results are positive (HDV RNA detected), or if symptoms such as jaundice, dark urine, or abdominal pain worsen despite negative results. Also consult if you have chronic Hepatitis B and suspect HDV exposure.
Limitations
- ⚠PCR may yield false negatives if viral load is very low or due to sampling errors.
- ⚠False positives can occur from laboratory contamination.
- ⚠Does not differentiate between acute and chronic infection; additional clinical correlation is needed.
- ⚠Cannot determine the stage of liver disease or viral genotype.
- ⚠Limited to detection of HDV RNA; antibody testing may be needed for comprehensive diagnosis.
Risks & Considerations
- ●Minor pain or bruising at the blood draw site.
- ●Rare risk of infection or excessive bleeding.
- ●No significant risks associated with the PCR analysis itself.
Interfering Factors
- ●Improper sample collection or handling, such as delayed separation of plasma.
- ●Contamination during sample processing.
- ●Hemolyzed or lipemic blood samples.
- ●Recent antiviral therapy may reduce viral load below detection limits.
- ●Storage conditions not met (e.g., improper temperature).
Compare With Similar Tests
| Test | Hepatitis D Virus Detection PCR Test | Hepatitis D Antibody Test | Hepatitis B Surface Antigen Test | Liver Function Test (LFT) | Hepatitis C Antibody Test |
|---|---|---|---|---|---|
| Comparison | Hepatitis D Virus Detection PCR Test |
Frequently Asked Questions
What is the Hepatitis D Virus Detection PCR Test?
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What sample is required for this test?
Is fasting required before the test?
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