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Human Immunodeficiency Virus 1 RNA Quantitative Real Time PCR with CD3 / CD4 (Immune Deficiency Panel 4) Markers Test

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Human Immunodeficiency Virus 1 RNA Quantitative Real Time PCR with CD3 / CD4 (Immune Deficiency Panel 4) Markers Test

Short Name: HIV-1 RNA PCR with CD3/CD4 Panel

Also known as: HIV Viral Load Test, CD3/CD4 Immune Deficiency Panel

Human Immunodeficiency Virus 1 RNA Quantitative Real Time PCR with CD3 / CD4 (Immune Deficiency Panel 4) Markers Test test available at DNA Labs India for ₹7,231. Uses Real Time RT-PCR, Flow Cytometry on Plasma for PCR, Whole blood for Flow Cytometry samples. Results in Reports are typically available within 2-3 business days, with sample collection on Wednesday or Saturday by 11 am and report delivery on Friday or Tuesday.. Free home collection in 300+ cities across India.

Molecular Diagnostics🏠 Home Collection

🩺 Medically Reviewed By

Overview

This test aids in the management of HIV-1 infected patients by quantifying viral load and assessing immune function through CD3/CD4 counts. It is used to monitor treatment response, guide therapy decisions, and evaluate disease progression, but not for initial HIV diagnosis.

Test Code
772
Price
₹7,231
Sample Type
Plasma for PCR, Whole blood for Flow Cytometry
Result Time
Reports are typically available within 2-3 business days, with sample collection on Wednesday or Saturday by 11 am and report delivery on Friday or Tuesday.
Fasting Required
Yes (12 hours)
Method
Real Time RT-PCR, Flow Cytometry
Step 1

Sample Collection

Fast for 12 hours before sample collection. Avoid strenuous activity and inform your doctor about any medications.

Method: Venipuncture

Step 2

Laboratory Analysis

Blood will be drawn from a vein in your arm by a trained phlebotomist using standard venipuncture technique.

Step 3

Report Delivery

Apply pressure to the puncture site to stop bleeding. Resume normal activities and drink plenty of fluids.

Timeline: Reports are typically available within 2-3 business days, with sample collection on Wednesday or Saturday by 11 am and report delivery on Friday or Tuesday.

Patient Instructions

1
Before the Test:Fast for 12 hours. Inform your doctor about any medications, recent illnesses, or vaccinations. Avoid strenuous activity before sample collection.
2
During the Test:The test involves a blood sample collection process that is quick, typically taking a few minutes, and is minimally invasive.
3
After the Test:You can resume normal activities immediately. Results will be available as per the turnaround time, usually within 2-3 business days.

About This Test

Who Should Get This Test

This test aids in the management of HIV-1 infected patients by quantifying viral load and assessing immune function through CD3/CD4 counts. It is used to monitor treatment response, guide therapy decisions, and evaluate disease progression, but not for initial HIV diagnosis.

How to Prepare

  • Ensure 12-hour fasting prior to test
  • Use correct tubes as specified: Lavender Top (EDTA) for PCR and Lavender Top (EDTA) and Green top (Sodium Heparin) for Flow Cytometry
  • Label samples accurately with patient details, time, and date
  • Ship samples as per temperature requirements: PCR samples refrigerated or frozen, Flow samples at 18-22°C without refrigeration or freezing

Doctor's Notes

Reviewed by — MBBS, MD (Microbiology) · Reg. No. 27670

"Regular monitoring of HIV viral load and CD4 counts is essential for effective management and treatment adjustment."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypePlasma for PCR, Whole blood for Flow Cytometry
Sample VolumePCR: 3 mL plasma; Flow: 4 mL whole blood
ContainerLavender Top (EDTA) tubes for PCR; Lavender Top (EDTA) and Green top (Sodium Heparin) tubes for Flow Cytometry
Collection MethodVenipuncture

Sample Stability

Plasma for PCR: stable refrigerated for 24 hours or frozen for longer storage
Whole blood for Flow: stable at room temperature (18-22°C) for up to 12 hours
Sample Rejection Criteria:
  • Hemolyzed or clotted samples
  • Incorrect tube type used
  • Insufficient sample volume
  • Unlabeled or mislabeled samples
  • Sample received beyond stability period

Understanding Your Results

Results should be interpreted by a qualified healthcare provider in the context of the patient's clinical history, symptoms, and other laboratory tests.
High HIV-1 RNA levels indicate active viral replication and may require treatment adjustment
Low or undetectable viral load suggests effective antiretroviral therapy
Low CD4 counts indicate compromised immune function and increased risk of infections
Monitor trends over time rather than relying on single values
Consult with an infectious disease specialist for personalized management based on results
⚠️ When to Consult a Doctor:

If your test results show abnormal viral load or CD4 counts, or if you experience symptoms of immune deficiency such as recurrent infections or unexplained weight loss, consult your doctor immediately.

Limitations

  • Not for initial diagnosis of HIV
  • Requires experienced personnel for interpretation
  • Results may vary with sample quality
  • Cannot detect HIV antibodies or antigens directly

Risks & Considerations

  • Minimal risks from blood draw, such as bruising, soreness, or rare infection at the puncture site
  • No significant risks from the test itself beyond standard blood collection

Interfering Factors

  • Sample hemolysis
  • Recent vaccination or infection
  • Certain medications affecting immune cells
  • Improper sample storage or handling
  • Delay in sample processing

Compare With Similar Tests

TestHuman Immunodeficiency Virus 1 RNA Quantitative Real Time PCR with CD3 / CD4 (Immune Deficiency Panel 4) Markers TestHIV Antibody TestCD4 Count TestHIV RNA PCR Qualitative Test
ComparisonHuman Immunodeficiency Virus 1 RNA Quantitative Real Time PCR with CD3 / CD4 (Immune Deficiency Panel 4) Markers Test

Frequently Asked Questions

What is the HIV-1 RNA PCR with CD3/CD4 test used for?
This test is used to monitor HIV-1 infection by quantifying viral load (HIV-1 RNA) and assessing immune function through CD3 and CD4 cell counts. It helps in managing treatment and evaluating disease progression.
How is the test performed?
The test involves collecting two blood samples: 3 mL of plasma from EDTA tubes for PCR analysis and 4 mL of whole blood from EDTA and heparin tubes for flow cytometry to measure CD3 and CD4 counts.
What does the test measure?
It measures the quantity of HIV-1 RNA in the blood (viral load) and the counts of CD3 and CD4 T-cells, which are critical for immune system function.
Is fasting required for this test?
Yes, 12 hours of fasting is recommended before sample collection to ensure accurate results.
What is the cost of the HIV-1 RNA PCR with CD3/CD4 test?
The test costs INR 7230.6 at DNA Labs India, which includes free home sample collection across India.
How long does it take to get results?
Results are typically available within 2-3 business days. Sample collection on Wednesday or Saturday by 11 am, with report delivery on Friday or Tuesday.
Is home sample collection available for this test?
Yes, DNA Labs India offers free home sample collection for online bookings in numerous cities across India.
What is the difference between this test and an HIV antibody test?
This test quantifies viral load and immune cell counts, while an HIV antibody test detects antibodies to HIV and is used for initial screening. This test is for monitoring, not diagnosis.
How often should this test be done?
The frequency depends on clinical guidelines and your doctor's advice. Typically, it is done every 3-6 months for HIV patients on treatment to monitor viral load and immune status.
What do abnormal results mean?
Abnormal results, such as high viral load or low CD4 counts, may indicate disease progression, treatment failure, or compromised immunity. Consult your doctor for interpretation and next steps.
Are there any risks to this test?
Risks are minimal and related to blood draw, such as bruising or soreness. There are no significant risks from the test itself.
Can this test be used for initial diagnosis of HIV?
No, this test is not intended for initial diagnosis or confirmation of HIV infection. It is used for monitoring and management in already diagnosed patients.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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Your medical data is protected under Indian law.

Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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