Human Rotavirus (RNA Detection) Qualitative Test
Short Name: HRV RNA Qualitative
Also known as: Rotavirus RNA PCR, HRV PCR, Rotavirus Qualitative PCR
Human Rotavirus (RNA Detection) Qualitative Test test available at DNA Labs India for ₹8,160. Uses Real-Time PCR on Stool samples. Results in Reports are available on the 4th working day after sample collection. Email reports are sent within 48 hours of result availability, and phone consultation within 36 hours.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The purpose of the Human Rotavirus (RNA Detection) Qualitative Test is to confirm the presence of rotavirus RNA in stool samples, thereby diagnosing an active rotavirus infection. This test is particularly useful in clinical settings where rapid and accurate identification of the causative pathogen is essential for patient management. It helps differentiate rotavirus from other viral, bacterial, or parasitic causes of gastroenteritis, guiding appropriate treatment and infection control measures. The test is also valuable in epidemiological surveillance and outbreak investigations, as rotavirus is highly contagious and can spread rapidly in community settings such as daycare centers and hospitals. By detecting viral RNA, the test provides a definitive diagnosis even during the early stages of infection when the viral load may be low. This qualitative assay does not quantify the viral load but confirms the presence of the virus, which is sufficient for clinical decision-making in most cases. Early diagnosis enables prompt rehydration therapy, reduces the risk of severe dehydration, and helps prevent unnecessary antibiotic use, which is ineffective against viral infections.
- Test Code
- 6156
- CPT Code
- 87798
- ICD Code
- A08.0
- Price
- ₹8,160
- Sample Type
- Stool
- Result Time
- Reports are available on the 4th working day after sample collection. Email reports are sent within 48 hours of result availability, and phone consultation within 36 hours.
- Fasting Required
- No
- Method
- Real-Time PCR
Sample Collection
No fasting required. Collect stool sample in a sterile container. Inform your doctor about any recent rotavirus vaccination.
Method: Clean catch stool sample
Laboratory Analysis
Collect a small amount of stool (about 5-10 grams) using the provided scoop. Avoid mixing urine or water with the sample.
Report Delivery
Seal the container tightly and label it with your name and date. Store the sample in the refrigerator (2-8°C) if not delivered immediately. Transport to the lab as soon as possible.
Timeline: Reports are available on the 4th working day after sample collection. Email reports are sent within 48 hours of result availability, and phone consultation within 36 hours.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of the Human Rotavirus (RNA Detection) Qualitative Test is to confirm the presence of rotavirus RNA in stool samples, thereby diagnosing an active rotavirus infection. This test is particularly useful in clinical settings where rapid and accurate identification of the causative pathogen is essential for patient management. It helps differentiate rotavirus from other viral, bacterial, or parasitic causes of gastroenteritis, guiding appropriate treatment and infection control measures. The test is also valuable in epidemiological surveillance and outbreak investigations, as rotavirus is highly contagious and can spread rapidly in community settings such as daycare centers and hospitals. By detecting viral RNA, the test provides a definitive diagnosis even during the early stages of infection when the viral load may be low. This qualitative assay does not quantify the viral load but confirms the presence of the virus, which is sufficient for clinical decision-making in most cases. Early diagnosis enables prompt rehydration therapy, reduces the risk of severe dehydration, and helps prevent unnecessary antibiotic use, which is ineffective against viral infections.
How to Prepare
- Use the sterile container provided by the lab
- Collect stool directly into the container, avoiding contamination
- Do not overfill the container
- Keep the sample cool until delivery
- Deliver to the lab within 24 hours of collection
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Rotavirus is a leading cause of severe diarrhea in children. Early detection via PCR helps in prompt management and prevention of outbreaks."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample received in inappropriate container
- Sample contaminated with urine or water
- Sample not labeled correctly
- Sample stored at room temperature for more than 24 hours
- Insufficient quantity of stool
Understanding Your Results
Detected
Rotavirus RNA is present in the stool sample, indicating an active rotavirus infection. Clinical correlation with symptoms is recommended.
Action: Initiate supportive care, rehydration therapy, and infection control measures. Consider vaccination status.
Not Detected
Rotavirus RNA was not found in the sample. This suggests that rotavirus is not the cause of the gastroenteritis symptoms.
Action: Consider other etiologies such as norovirus, adenovirus, or bacterial pathogens.
Consult a doctor if you or your child experience severe diarrhea, vomiting, signs of dehydration (dry mouth, decreased urination, lethargy), or if symptoms persist beyond 3 days. Immediate medical attention is needed for infants, young children, and immunocompromised individuals.
Limitations
- ⚠Qualitative test does not differentiate between vaccine strain and wild-type virus
- ⚠May not detect non-group A rotavirus strains
- ⚠False negatives possible if sample collected after viral shedding has ceased
- ⚠Requires specialized laboratory equipment and trained personnel
Risks & Considerations
- ●No significant risks associated with stool sample collection
- ●Potential for false positive due to recent vaccination
- ●Potential for false negative if sample is collected late in infection
Interfering Factors
- ●Improper sample collection or storage may degrade RNA
- ●Presence of PCR inhibitors in stool (e.g., bile salts, polysaccharides)
- ●Recent rotavirus vaccination (may yield positive result due to live attenuated vaccine strain)
- ●Cross-contamination during sample handling
Compare With Similar Tests
| Test | Human Rotavirus (RNA Detection) Qualitative Test | Rotavirus Antigen Test | Rotavirus RNA Detection (PCR) |
|---|---|---|---|
| Comparison | Human Rotavirus (RNA Detection) Qualitative Test |
Frequently Asked Questions
What is the cost of the Human Rotavirus RNA Detection Test?
What sample is required for this test?
Do I need to fast before the test?
How long does it take to get results?
Is home sample collection available?
What does a positive result mean?
Can this test detect all types of rotavirus?
Is this test covered by insurance?
What is the sensitivity of this test?
Can recent rotavirus vaccination affect the result?
How should I store the stool sample before delivery?
What are the symptoms of rotavirus infection?
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