Hydroxyproline Plasma Test
Also known as: Hydroxyproline Blood Test, Plasma Hydroxyproline Assay, Collagen Breakdown Marker
Hydroxyproline Plasma Test test available at DNA Labs India for ₹7,500. Uses LC-MS/MS on Plasma samples. Results in Reports are typically available within 3 days after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SHAILAJA RAGHUNATH MURDESHWAR
Consultant Physician · Reg: 8052
Last reviewed: September 7, 2026
Overview
The purpose of the hydroxyproline plasma test is to measure hydroxyproline levels in the blood to evaluate collagen metabolism and diagnose disorders associated with abnormal collagen breakdown, such as osteoporosis and arthritis.
- Test Code
- 844
- Price
- ₹7,500
- Sample Type
- Plasma
- Result Time
- Reports are typically available within 3 days after sample collection.
- Fasting Required
- Yes (12 hours)
- Method
- LC-MS/MS
Sample Collection
Patient must fast overnight (8-12 hours). Inform the healthcare provider about any medications, supplements, or recent illnesses. Avoid strenuous exercise before the test.
Method: Venipuncture
Laboratory Analysis
A blood sample is drawn from a vein in the arm using a needle and collected in a sodium heparin tube. The procedure takes a few minutes with minimal discomfort.
Report Delivery
Apply pressure to the puncture site with a cotton ball or bandage to prevent bleeding. Resume normal activities unless advised otherwise. Monitor for any signs of infection or prolonged bleeding.
Timeline: Reports are typically available within 3 days after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of the hydroxyproline plasma test is to measure hydroxyproline levels in the blood to evaluate collagen metabolism and diagnose disorders associated with abnormal collagen breakdown, such as osteoporosis and arthritis.
How to Prepare
- Overnight fasting is mandatory for adults and older children.
- Non-fasting samples are acceptable for pediatric patients.
- Collect 2 mL of blood in a sodium heparin (green top) tube.
- Separate plasma from cells within 2 hours of collection.
- Transfer plasma to a sterile screw-capped vial.
- Ship sample refrigerated (2-8°C) or frozen (-20°C) to maintain stability.
Doctor's Notes
Reviewed by Dr SHAILAJA RAGHUNATH MURDESHWAR — MBBS, MD (General Medicine) · Reg. No. 8052
"This test is valuable for assessing collagen metabolism and can aid in the early detection of bone and connective tissue disorders."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Hemolyzed or lipemic samples
- Incorrect sample type (not sodium heparin plasma)
- Insufficient sample volume
- Sample not processed or shipped within stability time
Understanding Your Results
May indicate conditions such as osteoporosis, arthritis, hyperparathyroidism, Paget's disease, or collagen degradation due to trauma or vitamin C deficiency.
Range: Elevated
Suggests normal collagen metabolism and no significant breakdown.
Range: Normal
Rarely seen; could indicate reduced collagen synthesis, but clinical correlation is needed.
Range: Low
Consult a doctor if you experience symptoms like persistent joint pain, bone fractures, or if your test results are abnormal. Also, consult before and after the test for proper guidance.
Limitations
- ⚠Not specific to a single disease; elevated levels can be seen in multiple conditions
- ⚠May require correlation with other clinical findings and tests
- ⚠Reference ranges can vary between laboratories
Risks & Considerations
- ●Minor bruising or pain at the puncture site
- ●Rare risk of infection
- ●Possibility of fainting or dizziness during blood draw
Interfering Factors
- ●Intake of collagen-rich foods or supplements
- ●Vitamin C deficiency or excess
- ●Certain medications like corticosteroids
- ●Recent injury or surgery affecting collagen turnover
- ●Pediatric age group may have different ranges
Compare With Similar Tests
| Test | Hydroxyproline Plasma Test | Urine Hydroxyproline Test | Bone Specific Alkaline Phosphatase | Collagen Type I C-Telopeptide (CTx) | Vitamin C Level Test |
|---|---|---|---|---|---|
| Comparison | Hydroxyproline Plasma Test | Urine test measures hydroxyproline excretion over 24 hours, which can be more indicative of collagen breakdown but requires urine collection. Plasma test is a single blood draw. | This is another bone turnover marker. Hydroxyproline is more specific to collagen, while bone-specific alkaline phosphatase indicates osteoblast activity. | CTx is a marker of bone resorption. Both tests assess bone metabolism but from different angles. | Since vitamin C deficiency can affect collagen synthesis, this test may be done alongside hydroxyproline test for a comprehensive assessment. |
Frequently Asked Questions
What is a Hydroxyproline Plasma Test?
Why is the Hydroxyproline Plasma Test performed?
What is the cost of the Hydroxyproline Plasma Test in India?
How should I prepare for the test?
What does an elevated hydroxyproline level indicate?
Is fasting required for the Hydroxyproline Plasma Test?
How is the sample collected for this test?
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