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Immunofixation Electrophoresis (IFE) 24 Hour Urine Test

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Immunofixation Electrophoresis (IFE) 24 Hour Urine Test

Immunofixation Electrophoresis (IFE) 24 Hour Urine Test test available at DNA Labs India for ₹11,000. Uses Agarose Gel Electrophoresis, Immunofixation on 24-hour urine aliquot without preservative samples. Results in 4 working days. Free home collection in 300+ cities across India.

Diagnostic🏠 Home Collection

🩺 Medically Reviewed By

Overview

To diagnose and monitor monoclonal gammopathies by detecting abnormal monoclonal proteins in urine, aiding in the management of conditions like multiple myeloma.

Test Code
876
Price
₹11,000
Sample Type
24-hour urine aliquot without preservative
Result Time
4 working days
Fasting Required
No
Method
Agarose Gel Electrophoresis, Immunofixation
Step 1

Sample Collection

No special preparation required, but follow 24-hour urine collection instructions carefully.

Method: 24-hour urine collection

Step 2

Laboratory Analysis

Collect all urine over 24 hours in a provided container, record total volume.

Step 3

Report Delivery

Ship sample refrigerated or frozen to maintain stability.

Timeline: 4 working days

Patient Instructions

1
Before the Test:No special preparation required; ensure proper 24-hour urine collection.
2
During the Test:Urine sample collected over 24 hours as per instructions.
3
After the Test:No restrictions; resume normal activities.

About This Test

Who Should Get This Test

To diagnose and monitor monoclonal gammopathies by detecting abnormal monoclonal proteins in urine, aiding in the management of conditions like multiple myeloma.

How to Prepare

  • Begin collection by discarding the first morning urine on day one.
  • Collect all subsequent urine for 24 hours in the container.
  • Record the exact 24-hour volume on the request form and container.
  • Mix well and aliquot 20 mL (minimum 10 mL) for testing.
  • Ship refrigerated or frozen to the lab promptly.

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"This test is crucial for early detection and monitoring of monoclonal gammopathies, aiding in timely intervention for conditions like multiple myeloma."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample Type24-hour urine aliquot without preservative
Sample Volume20 mL (10 mL min.)
ContainerUrine container without preservative
Collection Method24-hour urine collection

Sample Stability

Room Temperature
Refrigerator (2-8°C)
Frozen (-20°C or below)

Understanding Your Results

Results indicate presence or absence of monoclonal proteins in urine, which can confirm diagnoses like multiple myeloma or MGUS.
📊

Monoclonal protein detected

Suggests a monoclonal gammopathy; further evaluation needed for conditions like multiple myeloma.

📊

No monoclonal protein detected

Normal finding; no evidence of monoclonal gammopathy in urine.

⚠️ When to Consult a Doctor:

If you experience symptoms like proteinuria, hematuria, or swelling, or if you have a known monoclonal gammopathy for monitoring.

Limitations

  • Does not quantify monoclonal protein levels; used for detection and typing
  • May require confirmation with serum tests or other diagnostics
  • Not suitable for all types of proteinuria

Risks & Considerations

  • No significant risks; minimal discomfort from urine collection.
  • Possible skin irritation from repeated cleaning during collection.

Interfering Factors

  • Improper urine collection or preservation
  • Contamination of sample
  • Recent high-protein diet or strenuous exercise (may affect protein levels)

Frequently Asked Questions

What is the IFE 24 Hour Urine Test?
It is a diagnostic test that analyzes proteins in a 24-hour urine sample to detect monoclonal proteins, helping diagnose conditions like multiple myeloma.
Why is this test ordered?
It is ordered when symptoms suggest monoclonal gammopathies, such as proteinuria, hematuria, or swelling, or for monitoring known conditions.
How is the sample collected?
Collect all urine over 24 hours in a container, record the volume, and aliquot 20 mL for shipping refrigerated or frozen.
Is fasting required?
No, there is no fasting requirement for this test.
What is the cost of the IFE 24 Hour Urine Test?
The cost is INR 11,000 in India, with free home sample collection available in many cities.
How long does it take to get results?
Results are typically available within 4 working days.
What conditions can this test diagnose?
It can diagnose multiple myeloma, Waldenström's macroglobulinemia, amyloidosis, and monoclonal gammopathy of undetermined significance (MGUS).
Are there any risks?
The test is non-invasive with minimal risks, such as possible skin irritation from collection procedures.
How should I prepare for the test?
No special preparation is needed, but ensure proper 24-hour urine collection as instructed.
What if monoclonal proteins are detected?
Detection suggests a monoclonal gammopathy; consult a doctor for further evaluation and management.
Is home sample collection available?
Yes, free home sample collection is offered for online bookings across India.
What is the difference between IFE and regular protein electrophoresis?
IFE is more sensitive and specific for identifying monoclonal proteins, making it essential for accurate diagnosis of monoclonal gammopathies.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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