Nicotine and Cotinine Urine Test
Short Name: Nicotine & Cotinine Test
Also known as: Cotinine Test, Nicotine Screening, Tobacco Exposure Test
Nicotine and Cotinine Urine Test test available at DNA Labs India for ₹14,000. Uses LC-MS/MS on Random urine samples. Results in Reports are typically available after 2-3 weeks from sample submission.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
The purpose of the Nicotine and Cotinine Urine Test is to quantitatively measure the levels of nicotine and cotinine in urine to determine tobacco exposure status. This helps in diagnosing nicotine dependence, monitoring cessation progress, and evaluating exposure in non-smokers.
- Test Code
- 1311
- Price
- ₹14,000
- Sample Type
- Random urine
- Result Time
- Reports are typically available after 2-3 weeks from sample submission.
- Fasting Required
- No
- Method
- LC-MS/MS
Sample Collection
Ensure no nicotine products are used for 24 hours if testing for baseline levels, unless otherwise directed.
Method: Random urine collection
Laboratory Analysis
Collect a random urine sample in the provided sterile container.
Report Delivery
Label the container correctly and store as instructed. Ship refrigerated or frozen if necessary.
Timeline: Reports are typically available after 2-3 weeks from sample submission.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of the Nicotine and Cotinine Urine Test is to quantitatively measure the levels of nicotine and cotinine in urine to determine tobacco exposure status. This helps in diagnosing nicotine dependence, monitoring cessation progress, and evaluating exposure in non-smokers.
How to Prepare
- Use a sterile screw-capped container
- Collect at least 10 mL of urine
- Avoid contamination
- Ship samples refrigerated or frozen
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
"This urine test for nicotine and cotinine is essential for evaluating tobacco exposure, which can aid in developing personalized cessation strategies and monitoring treatment progress."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Insufficient volume
- Improper labeling
- Contaminated sample
- Leaking container
Understanding Your Results
No detectable nicotine or cotinine, suggesting no recent tobacco exposure.
Level: Negative
May indicate passive exposure or low-level use.
Level: Low Positive
Significant tobacco exposure, likely from active smoking or heavy use.
Level: Positive
If results are positive and you are trying to quit tobacco, or if you have symptoms of tobacco-related health issues, consult a healthcare professional for guidance.
Limitations
- ⚠Cannot distinguish between active and passive smoking with certainty
- ⚠Results may be affected by individual metabolism
- ⚠Not suitable for detecting very low levels of exposure
Risks & Considerations
- ●No significant risks associated with urine collection
- ●Rare chance of minor discomfort during collection
Interfering Factors
- ●Use of nicotine patches or gum
- ●Passive exposure to tobacco smoke
- ●Recent consumption of nicotine-containing products
- ●Dietary factors affecting metabolism
Compare With Similar Tests
| Test | Nicotine and Cotinine Urine Test | Blood Nicotine Test | Saliva Cotinine Test | Breath Carbon Monoxide Test |
|---|---|---|---|---|
| Comparison | Nicotine and Cotinine Urine Test |
Frequently Asked Questions
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