Pan Fungal Detection PCR Test
Short Name: Pan Fungal Detection PCR
Also known as: Pan-Fungal PCR, Broad Range Fungal DNA PCR, Fungal DNA Detection Test
Pan Fungal Detection PCR Test test available at DNA Labs India for ₹6,500. Uses Polymerase Chain Reaction (PCR), Broad-range Fungal DNA Amplification on Whole blood, CSF, sputum, body fluids, tissue samples. Results in Samples accepted by Tuesday or Saturday before 11 am are reported on Thursday or Monday, respectively. Reports will be available online, via email, and on WhatsApp.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
This assay is useful for the detection of fungi as a causative organism of a disease. It aids in the diagnosis of invasive fungal infections by identifying the presence of fungal DNA in clinical samples.
- Test Code
- 3530
- Price
- ₹6,500
- Sample Type
- Whole blood, CSF, sputum, body fluids, tissue
- Result Time
- Samples accepted by Tuesday or Saturday before 11 am are reported on Thursday or Monday, respectively. Reports will be available online, via email, and on WhatsApp.
- Fasting Required
- No
- Method
- Polymerase Chain Reaction (PCR), Broad-range Fungal DNA Amplification
Sample Collection
No special preparation required. For best yield, collect specimen before starting antifungal treatment whenever clinically feasible.
Method: Venipuncture for blood; sterile collection for CSF, sputum, fluids, or tissue biopsy
Laboratory Analysis
Strict sterile technique should be used. For blood, collect 4 mL in EDTA (lavender top). For fluids, use sterile screw-capped containers. For tissue, place in normal saline in a sterile container.
Report Delivery
Specimens must be refrigerated (2-8°C) and shipped to the laboratory as soon as possible. Do not freeze.
Timeline: Samples accepted by Tuesday or Saturday before 11 am are reported on Thursday or Monday, respectively. Reports will be available online, via email, and on WhatsApp.
Patient Instructions
About This Test
Who Should Get This Test
This assay is useful for the detection of fungi as a causative organism of a disease. It aids in the diagnosis of invasive fungal infections by identifying the presence of fungal DNA in clinical samples.
How to Prepare
- Whole blood: 4 mL (2 mL min.) in lavender top (EDTA) tube.
- CSF/Sputum/Fluids: 2 mL in a sterile screw-capped container.
- Tissue: In normal saline in a sterile container.
- Ship refrigerated. DO NOT FREEZE.
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Early detection of fungal DNA is critical for timely management of invasive fungal infections, particularly in immunocompromised patients."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Frozen or hemolyzed blood samples
- Insufficient volume (< 2 mL blood or < 1 mL fluid)
- Non-sterile containers for sterile site specimens
- Samples received after prolonged transport without refrigeration
- Improperly labeled or unlabeled specimens
Understanding Your Results
Positive
Fungal DNA detected. Indicates presence of a fungal pathogen. Clinical correlation and species identification are recommended.
Negative
No fungal DNA detected. Does not exclude fungal infection; if clinical suspicion is high, further testing or repeat testing may be advised.
If you have persistent fever, unexplained respiratory symptoms, or an underlying immunosuppressive condition, and your Pan Fungal Detection PCR result is positive, contact your treating physician promptly. Even a negative result should be discussed in the context of ongoing symptoms.
Limitations
- ⚠Pan-fungal PCR does not differentiate between colonization and true infection in non-sterile sites.
- ⚠Detection is qualitative; it does not quantify fungal burden.
- ⚠Negative result does not completely rule out active fungal infection.
- ⚠Species-level identification usually requires additional tests, such as DNA sequencing or culture.
Risks & Considerations
- ●No serious risks for blood collection. Mild pain, bruising, or dizziness may occur.
- ●If CSF collection is required, there are procedural risks (e.g., headache, infection, bleeding) that your physician will explain.
Interfering Factors
- ●Concurrent use of antifungal therapy may reduce fungal DNA load and cause false-negative results.
- ●Contamination of samples with environmental fungal spores during collection or handling.
- ●Presence of PCR inhibitors (e.g., haemoglobin, heparin, or certain culture media).
- ●Incorrect storage or delay in transport (extensive hemolysis, prolonged room temperature storage).
Compare With Similar Tests
| Test | Pan Fungal Detection PCR Test | Fungal Culture | KOH Mount | Galactomannan Antigen | Beta-D-Glucan Assay | ITS Gene Sequencing |
|---|---|---|---|---|---|---|
| Comparison | Pan Fungal Detection PCR Test |
Frequently Asked Questions
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