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PNH Comprehensive Work Up (Flow Cytometry)-Flaer, CD14, CD15, CD24, CD45,CD59, CD64, GlY-A Test

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PNH Comprehensive Work Up (Flow Cytometry)-Flaer, CD14, CD15, CD24, CD45,CD59, CD64, GlY-A Test

Also known as: PNH Flow Cytometry Panel, FLAER Test for PNH, GPI-Anchor Deficiency Test

PNH Comprehensive Work Up (Flow Cytometry)-Flaer, CD14, CD15, CD24, CD45,CD59, CD64, GlY-A Test test available at DNA Labs India for ₹12,000. Uses Flow Cytometry on Peripheral blood samples. Results in 1-2 days. Free home collection in 300+ cities across India.

Flow CytometryAdults🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

To diagnose Paroxysmal Nocturnal Hemoglobinuria (PNH) by detecting GPI-anchored protein deficiencies via flow cytometry, monitor disease progression, and guide treatment decisions.

Test Code
3157
Price
₹12,000
Sample Type
Peripheral blood
Result Time
1-2 days
Fasting Required
No
Method
Flow Cytometry
Step 1

Sample Collection

A doctor's prescription is required for this test, except for surgery, pregnancy cases, or individuals planning to travel abroad. No fasting is needed. Inform the lab about any medications or recent transfusions.

Method: Venipuncture

Step 2

Laboratory Analysis

A blood sample will be drawn from a vein in your arm using standard venipuncture. The procedure is quick and minimally invasive.

Step 3

Report Delivery

Apply pressure to the puncture site to prevent bruising. Resume normal activities unless advised otherwise.

Timeline: 1-2 days

Patient Instructions

1
Before the Test:Obtain a doctor's prescription. No fasting required. Inform the lab about any health conditions or medications.
2
During the Test:A small blood sample is drawn from your arm. The process takes about 10-15 minutes.
3
After the Test:You can resume normal activities immediately. Apply pressure to the puncture site if needed.

About This Test

Who Should Get This Test

To diagnose Paroxysmal Nocturnal Hemoglobinuria (PNH) by detecting GPI-anchored protein deficiencies via flow cytometry, monitor disease progression, and guide treatment decisions.

How to Prepare

  • No fasting required
  • Bring a valid doctor's prescription
  • Avoid strenuous exercise before the test
  • Stay hydrated
  • Wear loose clothing for easy access

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"PNH is a rare but serious blood disorder requiring precise diagnosis for effective management. This comprehensive flow cytometry test provides high sensitivity and specificity, aiding in early detection and monitoring to prevent complications like thrombosis and organ damage."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypePeripheral blood
Sample Volume2 ml
ContainerEDTA Vacutainer (2ml) or Sodium heparin Vacutainer (2ml)
Collection MethodVenipuncture

Sample Stability

Sample should be processed within 24 hours of collection
Store at ambient temperature or with cool pack during transport
Sample Rejection Criteria:
  • Hemolyzed or clotted sample
  • Incorrect container type
  • Insufficient sample volume
  • Missing patient information or prescription

Understanding Your Results

Results are interpreted based on the percentage of cells lacking GPI-anchored proteins. A negative result indicates no PNH clone, while positive results show varying clone sizes, guiding clinical management.
📊

Negative

No significant PNH clone detected (<0.5% GPI-deficient cells)

📊

Small PNH clone

0.5-10% GPI-deficient cells; may require monitoring

📊

Large PNH clone

>10% GPI-deficient cells; associated with higher risk of complications and need for treatment

📊

Intermediate

1-10% GPI-deficient cells; clinical correlation recommended

⚠️ When to Consult a Doctor:

Consult a hematologist or internal medicine specialist if you experience persistent symptoms like fatigue, dark urine, or unexplained blood clots, or if test results indicate PNH.

Limitations

  • ⚠May not detect very small PNH clones (<0.1%)
  • ⚠Requires expert interpretation by a hematopathologist
  • ⚠Cannot distinguish between PNH and other GPI-deficiency disorders without clinical correlation
  • ⚠Results may be affected by sample quality

Risks & Considerations

  • ●Minor bruising or pain at the puncture site
  • ●Rare risk of infection or fainting
  • ●No significant long-term risks

Interfering Factors

  • ●Recent blood transfusions may mask PNH clones
  • ●Sample hemolysis or improper storage
  • ●Use of complement inhibitors (e.g., eculizumab) can affect results
  • ●Concurrent infections or inflammatory conditions

Frequently Asked Questions

What is PNH?
Paroxysmal Nocturnal Hemoglobinuria (PNH) is a rare blood disorder where red blood cells break down prematurely due to a lack of protective proteins, leading to anemia and other complications.
Why is the PNH Comprehensive Work Up needed?
It provides an accurate diagnosis of PNH using multiple flow cytometry markers, helping to confirm the disease and guide treatment, especially when symptoms are vague or overlap with other conditions.
What markers are included in this test?
The test includes Flaer, CD14, CD15, CD24, CD45, CD59, CD64, and Gly-A to detect GPI-anchored protein deficiencies in blood cells.
How is the test performed?
A blood sample is drawn and analyzed using flow cytometry, a laser-based technique that counts and characterizes cells based on marker expression.
Is fasting required for this test?
No, fasting is not required. You can eat and drink normally before the test.
What is the cost of the test?
The test costs INR 12000, which includes home sample collection across India.
How long does it take to get results?
Results are typically available within 1-2 days after sample collection.
What do the results mean?
Results show the percentage of cells lacking GPI-anchored proteins. A negative result means no PNH clone, while positive results indicate PNH with varying clone sizes.
Are there any risks associated with the test?
The test involves a standard blood draw with minimal risks like bruising or discomfort. Serious complications are rare.
Can this test be done at home?
Yes, DNA Labs India offers free home sample collection for online bookings across many cities in India.
What should I do if my test is positive?
Consult a hematologist immediately for further evaluation and management, as PNH requires specialized care to prevent complications.
Is this test covered by insurance?
Coverage depends on your insurance policy. It is not typically covered under government schemes like PMJAY, but check with your provider for details.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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