Pseudomonas Aeruginosa (RNA Detection) Qualitative Test
Short Name: Pseudomonas RNA Qualitative
Also known as: Pseudomonas aeruginosa RNA PCR, Pseudomonas aeruginosa qualitative RNA test
Pseudomonas Aeruginosa (RNA Detection) Qualitative Test test available at DNA Labs India for ₹5,780. Uses Real Time RT-PCR on Sputum or throat swab (in cystic fibrosis patients), water samples samples. Results in Reports are available on the 4th working day. For urgent needs, email reports are sent within 48 hours and phone consultation within 36 hours.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The purpose of this test is to qualitatively detect the presence of Pseudomonas aeruginosa RNA in clinical specimens. It is used to confirm active infection, especially in patients with suspected respiratory tract infections, cystic fibrosis exacerbations, or sepsis. Early detection allows for timely antibiotic therapy, reducing the risk of severe complications and antibiotic resistance.
- Test Code
- 6227
- CPT Code
- 87798
- ICD Code
- B96.5
- Price
- ₹5,780
- Sample Type
- Sputum or throat swab (in cystic fibrosis patients), water samples
- Result Time
- Reports are available on the 4th working day. For urgent needs, email reports are sent within 48 hours and phone consultation within 36 hours.
- Fasting Required
- No
- Method
- Real Time RT-PCR
Sample Collection
No special preparation is required. However, patients should sign a consent document and provide relevant clinical history. For sputum collection, patients may be advised to rinse mouth with water before expectoration.
Method: Expectoration or swab
Laboratory Analysis
For sputum, the patient is asked to cough deeply to produce sputum from the lungs. For throat swab, a sterile swab is gently rubbed over the back of the throat. The sample is placed in a sterile container and transported to the laboratory immediately.
Report Delivery
The sample is sent to the laboratory for processing. Patients can resume normal activities immediately. Results are typically available within 4 working days.
Timeline: Reports are available on the 4th working day. For urgent needs, email reports are sent within 48 hours and phone consultation within 36 hours.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of this test is to qualitatively detect the presence of Pseudomonas aeruginosa RNA in clinical specimens. It is used to confirm active infection, especially in patients with suspected respiratory tract infections, cystic fibrosis exacerbations, or sepsis. Early detection allows for timely antibiotic therapy, reducing the risk of severe complications and antibiotic resistance.
How to Prepare
- For sputum: collect early morning sample after rinsing mouth with water
- For throat swab: use sterile swab, avoid touching tongue or cheeks
- For water samples: collect in sterile container, ensure no contamination
- Label the sample with patient name, date, and time of collection
- Transport sample to laboratory immediately or refrigerate if delay
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Pseudomonas aeruginosa is a notorious opportunistic pathogen, especially in immunocompromised and cystic fibrosis patients. Early and accurate detection is critical for initiating targeted antimicrobial therapy and preventing severe complications."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Insufficient sample volume
- Sample leaking or not properly sealed
- Sample not labeled correctly
- Sample received after prolonged delay without proper storage
Understanding Your Results
Detected
Pseudomonas aeruginosa RNA is present. This indicates active infection or colonization. Clinical correlation with symptoms and other tests is required. Antibiotic susceptibility testing may be recommended.
Not Detected
Pseudomonas aeruginosa RNA was not found in the sample. This suggests no current infection, but if clinical suspicion remains high, repeat testing or alternative diagnostic methods may be considered.
If you experience symptoms such as persistent fever, cough with purulent sputum, difficulty breathing, or signs of sepsis, consult a healthcare provider immediately. Early diagnosis and treatment are essential, especially for immunocompromised individuals.
Limitations
- ⚠Qualitative test does not provide bacterial load or quantification
- ⚠May not distinguish between active infection and colonization
- ⚠Negative result does not completely rule out infection if sample quality is poor
- ⚠Requires specialized laboratory equipment and trained personnel
Risks & Considerations
- ●No significant risks associated with sample collection
- ●Minor discomfort during throat swab
- ●Rare risk of coughing during sputum collection
Interfering Factors
- ●Improper sample collection or handling may lead to false negatives
- ●Inhibition of PCR by substances in the sample (e.g., blood, mucus)
- ●Recent antibiotic therapy may reduce bacterial load
- ●Contamination during collection can cause false positives
Compare With Similar Tests
| Test | Pseudomonas Aeruginosa (RNA Detection) Qualitative Test | Culture and Sensitivity | Antigen Detection | PCR (DNA) |
|---|---|---|---|---|
| Comparison | Pseudomonas Aeruginosa (RNA Detection) Qualitative Test |
Frequently Asked Questions
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