RNA Extraction from Blood-Paxgene/Tempus Test
Short Name: RNA Extraction (Paxgene/Tempus)
Also known as: RNA Isolation from Blood, Paxgene RNA Extraction, Tempus RNA Extraction
RNA Extraction from Blood-Paxgene/Tempus Test test available at DNA Labs India for ₹2,500. Uses RNA isolation using PAXgene/Tempus kit, Spectrophotometry, RNA integrity assessment on Blood (Paxgene or Tempus tube) samples. Results in Reports are typically delivered within 1 week after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The primary purpose of RNA extraction from blood is to isolate total RNA for molecular analysis. This is essential for diagnosing genetic disorders, identifying gene mutations associated with cancers, and studying gene expression changes in various diseases such as Alzheimer's, Parkinson's, and cardiovascular conditions. Additionally, it is used to monitor disease progression and evaluate the effectiveness of therapeutic interventions by analyzing RNA expression patterns over time.
- Test Code
- 6411
- CPT Code
- S3708
- ICD Code
- Z01.89
- Price
- ₹2,500
- Sample Type
- Blood (Paxgene or Tempus tube)
- Result Time
- Reports are typically delivered within 1 week after sample collection.
- Fasting Required
- No
- Method
- RNA isolation using PAXgene/Tempus kit, Spectrophotometry, RNA integrity assessment
Sample Collection
No special preparation is required. However, inform your healthcare provider about any medications or supplements you are taking, as they may influence gene expression.
Method: Venipuncture
Laboratory Analysis
A blood sample will be drawn from a vein in your arm using a sterile needle. The blood will be collected directly into a PAXgene or Tempus tube, which contains a stabilizing solution to preserve RNA.
Report Delivery
You can resume normal activities immediately. The sample will be transported to the laboratory under controlled conditions to ensure RNA stability.
Timeline: Reports are typically delivered within 1 week after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of RNA extraction from blood is to isolate total RNA for molecular analysis. This is essential for diagnosing genetic disorders, identifying gene mutations associated with cancers, and studying gene expression changes in various diseases such as Alzheimer's, Parkinson's, and cardiovascular conditions. Additionally, it is used to monitor disease progression and evaluate the effectiveness of therapeutic interventions by analyzing RNA expression patterns over time.
How to Prepare
- Use only PAXgene or Tempus Blood RNA tubes for collection
- Fill the tube to the indicated line to ensure proper RNA stabilization
- Invert the tube gently 8-10 times after collection to mix blood with the reagent
- Store the tube at room temperature (18-25°C) for up to 3 days or at -20°C for longer storage
- Do not refrigerate the tube, as this may cause hemolysis
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"RNA extraction from blood is a critical pre-analytical step for accurate gene expression profiling. Proper stabilization using Paxgene or Tempus tubes ensures RNA integrity, which is essential for reliable downstream molecular diagnostics."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Clotted or hemolyzed sample
- Incorrect tube type
- Insufficient blood volume
- Sample stored beyond recommended time or temperature
Understanding Your Results
A yield of ≥1 μg is generally sufficient for most downstream applications. Lower yields may require amplification or may indicate poor sample quality.
A RIN of ≥7 indicates high-quality RNA suitable for RNA sequencing and gene expression analysis. Lower RIN values suggest degradation and may compromise results.
A ratio between 1.8 and 2.1 indicates pure RNA. Values below 1.8 may suggest protein contamination, while higher values may indicate residual reagents.
A ratio ≥1.5 indicates minimal contamination with chaotropic salts or organic compounds. Lower values may affect downstream enzymatic reactions.
Consult your healthcare provider if you have symptoms suggestive of a genetic disorder, cancer, or infectious disease, or if you require RNA-based testing for diagnosis or treatment monitoring. They can guide you on the appropriate tests and interpretation of results.
Limitations
- ⚠RNA is highly susceptible to degradation; strict handling is required
- ⚠The test provides RNA, not diagnostic results; further analysis is needed
- ⚠Not suitable for formalin-fixed paraffin-embedded (FFPE) samples
- ⚠Requires specialized equipment and trained personnel
Risks & Considerations
- ●Minor bruising or bleeding at the puncture site
- ●Dizziness or fainting during blood draw
- ●Infection (rare, if proper hygiene is not maintained)
Interfering Factors
- ●Hemolysis can degrade RNA and affect yield
- ●Improper storage or delayed processing may reduce RNA integrity
- ●Contamination with genomic DNA can affect downstream applications
- ●Use of incorrect blood collection tube may compromise RNA stability
Compare With Similar Tests
| Test | RNA Extraction from Blood-Paxgene/Tempus | ||
|---|---|---|---|
| Comparison | RNA Extraction from Blood-Paxgene/Tempus |
Frequently Asked Questions
What is RNA extraction from blood?
Why are PAXgene and Tempus tubes used?
What is the cost of RNA extraction from blood at DNA Labs India?
Do I need to fast before the test?
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How long does it take to get the results?
What is the sample volume required?
Can RNA extraction be done from other sample types?
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