RNA Extraction from Cultured Cells Test
Short Name: RNA Extraction
Also known as: RNA Isolation from Cultured Cells, Total RNA Extraction
RNA Extraction from Cultured Cells Test test available at DNA Labs India for ₹2,500. Uses Guanidinium thiocyanate-phenol-chloroform extraction, Column-based purification on Cultured cells samples. Results in Reports are delivered within 1 week after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The primary purpose of RNA extraction from cultured cells is to obtain pure, intact RNA for various molecular analyses. This includes quantifying gene expression levels, detecting alternative splicing, identifying RNA modifications, and supporting the development of RNA-based therapeutics and vaccines. In clinical diagnostics, RNA extraction aids in diagnosing RNA-related diseases such as certain cancers, neurological disorders, and viral infections. By isolating RNA, researchers can study the transcriptome and identify biomarkers that may indicate disease presence or progression. Additionally, RNA extraction is essential for validating gene editing experiments and monitoring the efficacy of RNA interference therapies. At DNA Labs India, we ensure that the extracted RNA meets the highest quality standards, with appropriate concentration and purity ratios (A260/A280), enabling reliable downstream applications. Our service is designed to support both research and clinical needs, providing a cost-effective solution for laboratories and healthcare providers.
- Test Code
- 6406
- CPT Code
- S3830
- ICD Code
- Z01.89
- Price
- ₹2,500
- Sample Type
- Cultured cells
- Result Time
- Reports are delivered within 1 week after sample collection.
- Fasting Required
- No
- Method
- Guanidinium thiocyanate-phenol-chloroform extraction, Column-based purification
Sample Collection
Ensure cultured cells are healthy and at the appropriate confluency. If using adherent cells, detach them properly. For suspension cells, ensure viability. Transport cells in a sterile container with RNA stabilization reagent if possible.
Method: Home collection or lab visit
Laboratory Analysis
The sample will be collected by a trained professional. For home collection, the phlebotomist will follow sterile procedures. The cultured cells will be placed in a sterile tube with RNA stabilization solution to prevent degradation.
Report Delivery
The sample should be transported to the laboratory immediately or stored at 2-8°C for short-term (up to 24 hours) or -20°C for longer storage. Avoid repeated freeze-thaw cycles.
Timeline: Reports are delivered within 1 week after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of RNA extraction from cultured cells is to obtain pure, intact RNA for various molecular analyses. This includes quantifying gene expression levels, detecting alternative splicing, identifying RNA modifications, and supporting the development of RNA-based therapeutics and vaccines. In clinical diagnostics, RNA extraction aids in diagnosing RNA-related diseases such as certain cancers, neurological disorders, and viral infections. By isolating RNA, researchers can study the transcriptome and identify biomarkers that may indicate disease presence or progression. Additionally, RNA extraction is essential for validating gene editing experiments and monitoring the efficacy of RNA interference therapies. At DNA Labs India, we ensure that the extracted RNA meets the highest quality standards, with appropriate concentration and purity ratios (A260/A280), enabling reliable downstream applications. Our service is designed to support both research and clinical needs, providing a cost-effective solution for laboratories and healthcare providers.
How to Prepare
- Use sterile equipment to avoid contamination
- Include RNA stabilization reagent in the collection tube
- Label the tube with patient ID and date
- Maintain cold chain during transport
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"RNA extraction is a foundational step in molecular diagnostics. Proper isolation ensures accurate downstream analysis, which is critical for identifying genetic and RNA-based disorders."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample with visible hemolysis or contamination
- Insufficient cell number (<1 million cells)
- Sample not stored properly leading to RNA degradation
- Incorrect labeling or missing patient information
Understanding Your Results
A concentration ≥ 50 ng/µL is generally sufficient for most downstream applications. Lower concentrations may require concentration steps.
A ratio between 1.8 and 2.1 indicates pure RNA. Ratios below 1.8 may suggest protein contamination, while above 2.1 may indicate residual reagents.
A RIN ≥ 7.0 indicates intact RNA suitable for sensitive assays like qPCR and sequencing. Lower RIN values suggest degradation.
A yield ≥ 5 µg is typically adequate for multiple experiments. Lower yields may limit the number of assays.
If you are undergoing RNA extraction for clinical diagnostic purposes, consult your referring physician or geneticist to understand the implications of the results. For research, consult your principal investigator or lab supervisor.
Limitations
- ⚠RNA is highly susceptible to degradation; improper handling may compromise quality
- ⚠The test does not provide functional information about RNA
- ⚠Results are dependent on the quality of the starting cultured cells
- ⚠Not a diagnostic test for specific diseases; downstream analysis required
Risks & Considerations
- ●No significant risks associated with this test as it is performed on cultured cells
- ●Potential for sample contamination if not handled properly
- ●RNA degradation if storage conditions are not maintained
Interfering Factors
- ●RNase contamination leading to RNA degradation
- ●Incomplete cell lysis affecting yield
- ●Carryover of genomic DNA
- ●Inhibitors like phenol or ethanol in the final eluate
- ●Improper storage conditions causing RNA instability
Compare With Similar Tests
| Test | RNA Extraction from Cultured Cells | ||
|---|---|---|---|
| Comparison | RNA Extraction from Cultured Cells |
Frequently Asked Questions
What is RNA extraction from cultured cells?
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₹20,000Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
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