RNA Extraction from Fungus-Mycelium Test
Short Name: RNA Extraction (Fungal)
Also known as: Fungal RNA Isolation, Mycelial RNA Extraction
RNA Extraction from Fungus-Mycelium Test test available at DNA Labs India for ₹2,500. Uses Phenol-chloroform extraction, Silica column purification, UV spectrophotometry on Fungal mycelium (from culture or infected tissue) samples. Results in Reports are typically delivered within 1 week after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The primary purpose of RNA extraction from fungus-mycelium is to obtain high-quality, intact RNA for molecular analysis. This is crucial for studying fungal gene expression, identifying virulence factors, and detecting drug resistance markers. In clinical settings, RNA-based assays can aid in the diagnosis of fungal infections, especially when culture is inconclusive or slow-growing. Additionally, RNA extraction is fundamental in research for functional genomics and understanding host-pathogen interactions.
- Test Code
- 6412
- CPT Code
- S3700
- ICD Code
- B49
- Price
- ₹2,500
- Sample Type
- Fungal mycelium (from culture or infected tissue)
- Result Time
- Reports are typically delivered within 1 week after sample collection.
- Fasting Required
- No
- Method
- Phenol-chloroform extraction, Silica column purification, UV spectrophotometry
Sample Collection
No special preparation is required. However, if the sample is from a clinical specimen, ensure it is collected under sterile conditions. For culture-based samples, the mycelium should be fresh and actively growing.
Method: Sterile collection by trained personnel
Laboratory Analysis
The sample should be collected using sterile instruments and placed immediately into a sterile container with appropriate transport medium. Avoid contamination with environmental fungi.
Report Delivery
The sample should be transported to the laboratory at 2-8°C if processing is delayed. For long-term storage, freeze at -80°C. Ensure proper labeling with patient details.
Timeline: Reports are typically delivered within 1 week after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of RNA extraction from fungus-mycelium is to obtain high-quality, intact RNA for molecular analysis. This is crucial for studying fungal gene expression, identifying virulence factors, and detecting drug resistance markers. In clinical settings, RNA-based assays can aid in the diagnosis of fungal infections, especially when culture is inconclusive or slow-growing. Additionally, RNA extraction is fundamental in research for functional genomics and understanding host-pathogen interactions.
How to Prepare
- Use sterile technique to avoid contamination
- Collect sufficient mycelial mass (at least 10 mg)
- Place sample in a sterile screw-cap tube
- Transport to laboratory within 24 hours if refrigerated
- Do not freeze without proper cryoprotectant
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"RNA extraction from fungal mycelium is a critical step in molecular diagnosis of fungal infections, enabling accurate identification and gene expression profiling. This test supports targeted antifungal therapy and monitoring of treatment response."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample with visible contamination
- Insufficient mycelial mass
- Sample not stored properly
- Sample with excessive degradation
- Unlabeled or mislabeled sample
Understanding Your Results
Sufficient RNA for most downstream applications
Recommendation: Proceed with RT-PCR or sequencing
Good purity with minimal protein contamination
Recommendation: Acceptable for molecular assays
High-quality RNA with minimal degradation
Recommendation: Suitable for gene expression analysis
May indicate degradation or contamination
Recommendation: Consider re-extraction or troubleshooting
If you have symptoms of a fungal infection such as persistent cough, fever, skin lesions, or nail changes, or if you have been advised by a physician to undergo this test, please consult your doctor for proper interpretation and management.
Limitations
- ⚠RNA is highly susceptible to degradation; requires immediate processing
- ⚠May not differentiate between viable and non-viable organisms
- ⚠Requires specialized equipment and trained personnel
- ⚠Results depend on sample quality and quantity
- ⚠Not a standalone diagnostic test; should be correlated with clinical findings
Risks & Considerations
- ●Minimal risk of bleeding or infection at the collection site (if tissue biopsy)
- ●No significant risks associated with the test itself
Interfering Factors
- ●Degradation of RNA due to improper storage or handling
- ●Contamination with genomic DNA
- ●Presence of PCR inhibitors in the sample
- ●Insufficient mycelial mass
- ●Excessive polysaccharides or polyphenols in fungal cells
Compare With Similar Tests
| Test | RNA Extraction from Fungus-Mycelium | Fungal Culture | PCR for Fungal DNA | Microscopic Examination |
|---|---|---|---|---|
| Comparison | RNA Extraction from Fungus-Mycelium | Culture identifies the fungus by growth on specific media, while RNA extraction provides molecular material for genetic analysis. Culture is slower (days to weeks) and may miss fastidious organisms. | PCR detects fungal DNA, while RNA extraction focuses on RNA, which reflects active gene expression. RNA is less stable but provides information on viability and expression. | Microscopy visualizes fungal elements directly, while RNA extraction requires molecular processing. Microscopy is quick but less specific. |
Frequently Asked Questions
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