RNA Extraction from Milk Test
Short Name: RNA Extraction Milk
Also known as: Milk RNA Isolation, RNA Isolation from Milk, Milk RNA Extraction
RNA Extraction from Milk Test test available at DNA Labs India for ₹3,000. Uses Phenol-chloroform extraction, Column-based purification on Milk samples. Results in Results are typically available within 1 week after sample collection. The report will include RNA yield, purity, and integrity metrics.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The purpose of RNA extraction from milk is to obtain high-quality RNA for various applications, including gene expression analysis, detection of microRNAs as potential biomarkers, identification of pathogens (e.g., viruses, bacteria) in milk, and research on lactation biology. It is also used in the diagnosis and monitoring of diseases such as breast cancer, where specific RNA signatures in milk may indicate early changes. The extracted RNA can be used for downstream applications like RT-PCR, qPCR, and next-generation sequencing, providing valuable insights into health and disease.
- Test Code
- 6417
- CPT Code
- S3830
- ICD Code
- Z01.89
- Price
- ₹3,000
- Sample Type
- Milk
- Result Time
- Results are typically available within 1 week after sample collection. The report will include RNA yield, purity, and integrity metrics.
- Fasting Required
- No
- Method
- Phenol-chloroform extraction, Column-based purification
Sample Collection
No special preparation is required. However, it is recommended to clean the breast and hands thoroughly before collection to minimize contamination. Use a sterile, RNase-free container provided by the lab.
Method: Manual expression or pump into sterile container
Laboratory Analysis
Collect 5-10 mL of milk into the sterile container. Avoid touching the inside of the container or the lid. If using a pump, ensure it is clean and RNase-free. Immediately close the container tightly and label it with your details.
Report Delivery
Store the sample in a refrigerator (2-8°C) if it cannot be delivered to the lab within 2 hours. For longer storage, freeze at -20°C or below. Do not add any preservatives unless instructed by the lab.
Timeline: Results are typically available within 1 week after sample collection. The report will include RNA yield, purity, and integrity metrics.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of RNA extraction from milk is to obtain high-quality RNA for various applications, including gene expression analysis, detection of microRNAs as potential biomarkers, identification of pathogens (e.g., viruses, bacteria) in milk, and research on lactation biology. It is also used in the diagnosis and monitoring of diseases such as breast cancer, where specific RNA signatures in milk may indicate early changes. The extracted RNA can be used for downstream applications like RT-PCR, qPCR, and next-generation sequencing, providing valuable insights into health and disease.
How to Prepare
- Wash hands and breast area with soap and water before collection
- Use a sterile, RNase-free container provided by DNA Labs India
- Collect mid-stream milk to avoid initial contamination
- Avoid touching the inside of the container or lid
- Label the container with your name, date, and time of collection
- Transport the sample to the lab as soon as possible, ideally within 2 hours
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"RNA extraction from milk is a specialized procedure that requires careful handling to preserve RNA integrity. It is primarily used in research settings, but has emerging clinical applications in non-invasive diagnostics. Proper sample collection and storage are critical to avoid degradation."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample not collected in RNase-free container
- Sample hemolyzed or contaminated with blood
- Sample stored improperly (e.g., left at room temperature for >2 hours)
- Insufficient volume (<2 mL)
- Sample not labeled correctly
Understanding Your Results
A yield of ≥10 ng/µL is generally considered adequate for most downstream applications. Lower yields may require concentration or may indicate poor extraction efficiency.
A ratio between 1.8 and 2.1 indicates pure RNA with minimal protein contamination. Ratios below 1.8 suggest protein contamination, while ratios above 2.1 may indicate phenol or other organic solvent contamination.
A RIN score of ≥7 indicates high-quality RNA suitable for sensitive applications like NGS. Lower scores suggest degradation, which may affect downstream results.
If you are undergoing RNA extraction from milk for clinical purposes, consult your physician to understand the implications of the results. For research purposes, consult the principal investigator or laboratory director for guidance on downstream analysis.
Limitations
- ⚠RNA is highly susceptible to degradation; requires immediate processing or proper storage
- ⚠Milk composition varies between individuals and lactation stages, affecting RNA yield
- ⚠Low abundance of certain RNA species may require amplification
- ⚠Not a diagnostic test for any specific disease; results require interpretation by qualified professionals
- ⚠Contamination can lead to false results in downstream applications
Risks & Considerations
- ●No significant risks associated with milk collection
- ●Mild discomfort during manual expression
- ●Risk of contamination if collection instructions are not followed
Interfering Factors
- ●Milk fat content can interfere with RNA extraction efficiency
- ●Presence of RNases from skin or environment can degrade RNA
- ●Improper storage temperature leading to RNA degradation
- ●Contamination with bacteria or somatic cells may affect RNA profile
- ●Insufficient sample volume may yield low RNA concentration
Compare With Similar Tests
| Test | RNA Extraction from Milk | RNA Extraction from Blood | RNA Extraction from Tissue |
|---|---|---|---|
| Comparison | RNA Extraction from Milk | Blood RNA extraction is more common and standardized, but milk offers a non-invasive alternative for certain studies. Milk RNA may have different composition and requires special handling due to fat content. | Tissue RNA extraction typically yields higher RNA concentration but requires invasive biopsy. Milk is non-invasive and can be collected repeatedly, making it suitable for longitudinal studies. |
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