RNA Extraction from Solid Tissue- Plant Test
Short Name: RNA Extraction (Plant)
Also known as: Plant RNA Isolation, RNA Purification from Plant Tissue
RNA Extraction from Solid Tissue- Plant Test test available at DNA Labs India for ₹2,500. Uses Column-based RNA isolation, Spectrophotometry, Gel electrophoresis on Solid tissue (plant) samples. Results in Reports are typically delivered within 1 week after sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The primary purpose of RNA extraction from solid plant tissue is to isolate high-quality RNA for downstream molecular analysis. This is essential for studying gene expression patterns, identifying disease-related biomarkers, understanding plant responses to environmental stress, and developing genetically modified crops. In clinical research, RNA extracted from plant tissues can be used to investigate plant-based pharmaceuticals and nutraceuticals. The extracted RNA serves as a template for reverse transcription and quantitative PCR, enabling accurate quantification of specific transcripts. Our service ensures that the RNA is free from inhibitors and degradation, which is critical for obtaining meaningful and reproducible results in gene expression studies.
- Test Code
- 6407
- CPT Code
- S3820
- ICD Code
- Z01.89
- Price
- ₹2,500
- Sample Type
- Solid tissue (plant)
- Result Time
- Reports are typically delivered within 1 week after sample receipt.
- Fasting Required
- No
- Method
- Column-based RNA isolation, Spectrophotometry, Gel electrophoresis
Sample Collection
No special preparation is required. However, it is recommended to collect fresh tissue and store it in an appropriate RNA stabilization solution (e.g., RNAlater) if immediate processing is not possible.
Method: Biopsy or surgical specimen
Laboratory Analysis
The sample should be collected using sterile, RNase-free tools. The tissue should be placed in a sterile container with RNA stabilization reagent and transported on ice to the laboratory.
Report Delivery
The sample should be delivered to the laboratory as soon as possible. If delayed, store at 2-8°C for up to 24 hours or at -20°C for longer storage.
Timeline: Reports are typically delivered within 1 week after sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of RNA extraction from solid plant tissue is to isolate high-quality RNA for downstream molecular analysis. This is essential for studying gene expression patterns, identifying disease-related biomarkers, understanding plant responses to environmental stress, and developing genetically modified crops. In clinical research, RNA extracted from plant tissues can be used to investigate plant-based pharmaceuticals and nutraceuticals. The extracted RNA serves as a template for reverse transcription and quantitative PCR, enabling accurate quantification of specific transcripts. Our service ensures that the RNA is free from inhibitors and degradation, which is critical for obtaining meaningful and reproducible results in gene expression studies.
How to Prepare
- Use sterile, RNase-free collection tubes
- Add RNA stabilization solution to the tube before placing the tissue
- Ensure the tissue is fully submerged in the solution
- Label the tube with patient/sample ID and date
- Transport on ice or at 4°C
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"RNA quality is critical for downstream gene expression studies. Our extraction protocol ensures high integrity RNA from plant tissues, minimizing degradation and contamination."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample not in RNA stabilization solution
- Sample received at room temperature after prolonged delay
- Insufficient tissue quantity
- Sample contaminated with soil or other foreign material
- Sample with visible degradation or fungal growth
Understanding Your Results
A yield of ≥10 µg indicates sufficient RNA for most downstream applications. Lower yields may require additional samples or amplification.
A ratio between 1.8 and 2.1 indicates pure RNA. Lower ratios suggest protein or phenol contamination, while higher ratios may indicate degradation.
A RIN score ≥7.0 indicates intact RNA suitable for sensitive applications like RNA-seq. Lower scores may lead to biased results.
Absence of DNA contamination is essential for accurate gene expression analysis. If present, additional DNase treatment may be required.
If you are a researcher or clinician planning to use RNA for diagnostic or therapeutic purposes, it is advisable to consult with a molecular biologist or geneticist to ensure proper sample handling and interpretation of results.
Limitations
- ⚠RNA quality may vary depending on tissue type and age
- ⚠Highly lignified or fibrous tissues may require special protocols
- ⚠Samples with low RNA content may yield insufficient quantities
- ⚠Results are dependent on sample integrity at the time of collection
Risks & Considerations
- ●No significant risks associated with sample collection, but if a biopsy is performed, there is a minimal risk of bleeding or infection.
Interfering Factors
- ●Presence of polyphenols and polysaccharides in plant tissues
- ●Improper sample storage leading to RNA degradation
- ●Contamination with RNase enzymes
- ●Insufficient tissue amount
- ●Excessive mechanical disruption causing shearing
Compare With Similar Tests
| Test | RNA Extraction from Solid Tissue- Plant | RNA Extraction from Blood | RNA Extraction from Animal Tissue | RNA Extraction from Cultured Cells |
|---|---|---|---|---|
| Comparison | RNA Extraction from Solid Tissue- Plant |
Frequently Asked Questions
What is the cost of RNA extraction from solid tissue (plant) at DNA Labs India?
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Is home sample collection available for this test?
What is the recommended amount of tissue for RNA extraction?
Can RNA be extracted from any type of plant tissue?
What quality checks are performed on the extracted RNA?
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