RNA QC and Quantitation Test
Short Name: RNA QC
Also known as: RNA Quality Control, RNA Quantitation, RNA Integrity Analysis
RNA QC and Quantitation Test test available at DNA Labs India for ₹850. Uses Spectrophotometry, Agarose Gel Electrophoresis, Capillary Electrophoresis on Extracted RNA samples. Results in Reports are typically delivered within 1 week after sample submission.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SULOCHANA HEMCHANDRA HOLLA
Consultant Medical Geneticist · Reg: 8532
Last reviewed: September 7, 2026
Overview
The primary purpose of RNA QC and quantitation is to evaluate the quality and quantity of RNA samples before proceeding with sensitive downstream applications. Poor RNA quality can lead to inaccurate gene expression data, failed sequencing runs, and wasted resources. This test helps researchers and clinicians confirm that RNA samples are intact, free from contaminants, and present in sufficient concentration for successful experiments. By identifying degradation, contamination, or low yield early, this service enables corrective actions, saving time and ensuring the integrity of your research outcomes.
- Test Code
- 6416
- CPT Code
- 83880
- ICD Code
- Z01.89
- Price
- ₹850
- Sample Type
- Extracted RNA
- Result Time
- Reports are typically delivered within 1 week after sample submission.
- Fasting Required
- No
- Method
- Spectrophotometry, Agarose Gel Electrophoresis, Capillary Electrophoresis
Sample Collection
Ensure RNA is extracted using a validated method and stored at -80°C. Avoid repeated freeze-thaw cycles. Provide sample in an RNase-free tube with clear labeling.
Method: Sample pickup or in-lab submission
Laboratory Analysis
Samples should be transported on dry ice to maintain integrity. If shipping, use a reliable courier with temperature control.
Report Delivery
No special precautions required. Results will be available within 1 week.
Timeline: Reports are typically delivered within 1 week after sample submission.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of RNA QC and quantitation is to evaluate the quality and quantity of RNA samples before proceeding with sensitive downstream applications. Poor RNA quality can lead to inaccurate gene expression data, failed sequencing runs, and wasted resources. This test helps researchers and clinicians confirm that RNA samples are intact, free from contaminants, and present in sufficient concentration for successful experiments. By identifying degradation, contamination, or low yield early, this service enables corrective actions, saving time and ensuring the integrity of your research outcomes.
How to Prepare
- Use RNase-free tubes and gloves
- Label the tube with sample ID and date
- Ship on dry ice or at -20°C if immediate delivery is not possible
- Include a completed requisition form
Doctor's Notes
Reviewed by Dr SULOCHANA HEMCHANDRA HOLLA — MBBS, MD (Medical Genetics) · Reg. No. 8532
"RNA quality is critical for accurate gene expression analysis. This test ensures your samples meet the highest standards for downstream applications."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample not labeled properly
- Sample in non-RNase-free tube
- Sample degraded (visible on gel)
- Insufficient volume for analysis
- Sample shipped at room temperature
Understanding Your Results
Excellent RNA quality, suitable for all downstream applications
RNA is intact but may require concentration or more starting material
Possible protein or phenol contamination, may need purification
RNA degradation, not recommended for sensitive applications
If you are a clinician or researcher and your RNA QC results indicate poor quality, consult with a molecular biology specialist or geneticist to determine the best course of action, such as re-extraction or troubleshooting.
Limitations
- ⚠Results depend on the quality of the extracted RNA provided
- ⚠Spectrophotometry may overestimate concentration if contaminants are present
- ⚠RIN values may be affected by tissue type and extraction method
- ⚠This test does not include RNA isolation or downstream applications
Risks & Considerations
- ●No significant risks as this is a laboratory test on extracted RNA
- ●Potential for sample loss if not handled properly
Interfering Factors
- ●Contamination with genomic DNA
- ●Presence of proteins or phenol
- ●RNA degradation due to improper storage
- ●Excessive salt or ethanol carryover
- ●Sample dilution errors
Compare With Similar Tests
| Test | RNA QC and Quantitation | DNA QC | Protein Quantitation | cDNA Synthesis |
|---|---|---|---|---|
| Comparison | RNA QC and Quantitation |
Frequently Asked Questions
What is RNA QC and quantitation?
Why is RNA quality important?
What is the cost of RNA QC and quantitation at DNA Labs India?
What sample type is required for this test?
How is RNA quality assessed?
What is a good RNA Integrity Number (RIN)?
How long does it take to get results?
Do I need to fast before this test?
Can I get a home sample collection?
What are the common symptoms of poor RNA quality?
Is this test covered by insurance?
What downstream applications can I use after RNA QC?
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Reference Laboratory Services
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