Sequencing Library QC-TapeStation Test
Short Name: Sequencing Library QC
Also known as: TapeStation QC, Library QC, NGS Library Quality Check
Sequencing Library QC-TapeStation Test test available at DNA Labs India for ₹950. Uses Agilent TapeStation Electrophoresis on Extracted DNA samples. Results in Reports are delivered within 4 days after sample collection.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Pasupathy Arumugam
Consultant Pathologist · Reg: 21521
Last reviewed: September 7, 2026
Overview
The purpose of the Sequencing Library QC-TapeStation test is to verify the quality and quantity of a sequencing library before it is loaded onto an NGS platform. It detects issues such as low yield, improper fragment size, adapter contamination, and sample degradation. By identifying these problems early, the test helps optimize sequencing efficiency, reduce costs, and ensure high-confidence variant calling. It is a mandatory quality checkpoint in genomics laboratories to maintain data integrity and reproducibility.
- Test Code
- 6436
- CPT Code
- 0000U
- ICD Code
- Z01.89
- Price
- ₹950
- Sample Type
- Extracted DNA
- Result Time
- Reports are delivered within 4 days after sample collection.
- Fasting Required
- No
- Method
- Agilent TapeStation Electrophoresis
Sample Collection
Ensure the extracted DNA/RNA is of high quality and free from contaminants. The library should be prepared according to the NGS kit manufacturer's instructions. No fasting is required.
Method: Pipetting of extracted library
Laboratory Analysis
The sample is pipetted into a designated well of the TapeStation chip. The process is automated and takes about 1-2 minutes per sample.
Report Delivery
No special precautions are needed. The sample is analyzed immediately, and the report is generated within 4 days.
Timeline: Reports are delivered within 4 days after sample collection.
Patient Instructions
About This Test
Who Should Get This Test
The purpose of the Sequencing Library QC-TapeStation test is to verify the quality and quantity of a sequencing library before it is loaded onto an NGS platform. It detects issues such as low yield, improper fragment size, adapter contamination, and sample degradation. By identifying these problems early, the test helps optimize sequencing efficiency, reduce costs, and ensure high-confidence variant calling. It is a mandatory quality checkpoint in genomics laboratories to maintain data integrity and reproducibility.
How to Prepare
- Provide the extracted DNA/RNA library in a clearly labeled tube
- Include the library concentration and expected size if known
- Avoid repeated freeze-thaw cycles
- Ship on dry ice if the sample is not analyzed immediately
Doctor's Notes
Reviewed by Dr Pasupathy Arumugam — MBBS, MD (Pathology) · Reg. No. 21521
"Sequencing library QC is a critical pre-sequencing step. A poor-quality library can lead to failed runs, wasted resources, and unreliable variant calls. This test ensures your library meets the stringent quality metrics required for accurate NGS results."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample not labeled correctly
- Insufficient volume (<1 µL)
- Visible contamination or precipitation
- Sample received in improper container
Understanding Your Results
Library is suitable for NGS. Proceed with sequencing.
May require re-amplification or concentration step. Check for loss during purification.
Indicates incomplete cleanup. Re-purify the library to remove dimers.
Suggests degradation or contamination. Re-evaluate library preparation.
If your sequencing library fails QC, consult your molecular biologist or geneticist to troubleshoot the library preparation protocol. For clinical samples, a genetic counselor may be involved to discuss the impact on test results.
Limitations
- ⚠This test is not a substitute for full sequencing quality metrics (e.g., Q30 scores)
- ⚠It does not detect all types of contaminants (e.g., protein)
- ⚠Results are dependent on the quality of the extracted DNA/RNA provided
- ⚠TapeStation analysis may not resolve very large fragments (>50 kb) accurately
Risks & Considerations
- ●No significant risks are associated with this test as it is performed on extracted DNA/RNA.
- ●Potential for sample loss if the volume is insufficient.
Interfering Factors
- ●Contamination with phenol, ethanol, or salts
- ●RNA contamination in DNA libraries
- ●Excessive freeze-thaw cycles of the library
- ●Improper storage temperature
- ●Insufficient sample volume
Compare With Similar Tests
| Test | Sequencing Library QC-TapeStation | ||
|---|---|---|---|
| Comparison | Sequencing Library QC-TapeStation |
Frequently Asked Questions
What is the cost of Sequencing Library QC-TapeStation at DNA Labs India?
What sample is required for this test?
How long does it take to get the report?
Is fasting required before the test?
Can I book a home sample collection?
What does the TapeStation measure?
Why is library QC important before NGS?
What is the reference range for library concentration?
Can this test detect adapter dimers?
Is this test NABL accredited?
What if my library fails QC?
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