Sperm DNA Fragmentation DFI Test
Also known as: Sperm DNA Fragmentation Index Test, Sperm DFI Test, Sperm DNA Integrity Test
Sperm DNA Fragmentation DFI Test test available at DNA Labs India for ₹8,000. Uses TUNEL Assay, Fluorescent Microscopy on Seminal Fluid samples. Results in Reports are issued within 7 working days and will be shared through the selected delivery mode such as email, WhatsApp or the online portal.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr SHAILAJA RAGHUNATH MURDESHWAR
Consultant Physician · Reg: 8052
Last reviewed: September 7, 2026
Overview
To assess sperm DNA integrity by measuring the percentage of sperm with fragmented DNA. The result helps identify a hidden male factor in couples with unexplained infertility, recurrent pregnancy loss, failed IVF/ICSI attempts, or known risk factors such as varicocele, smoking, scrotal heat exposure, and chronic infection.
- Test Code
- 3654
- Price
- ₹8,000
- Sample Type
- Seminal Fluid
- Result Time
- Reports are issued within 7 working days and will be shared through the selected delivery mode such as email, WhatsApp or the online portal.
- Fasting Required
- No
- Method
- TUNEL Assay, Fluorescent Microscopy
Sample Collection
No fasting is required. Follow the ejaculatory abstinence period advised by your doctor. Inform the laboratory about current medications, smoking, fever, or any infection.
Method: Semen sample collection
Laboratory Analysis
The semen sample is produced by masturbation into a sterile wide-mouth container. Do not use lubricants, spermicidal gels, or a regular condom as these may interfere with the test.
Report Delivery
Keep the container tightly closed and deliver it to the laboratory as per the advised schedule. Do not expose the sample to extreme heat or direct sunlight.
Timeline: Reports are issued within 7 working days and will be shared through the selected delivery mode such as email, WhatsApp or the online portal.
Patient Instructions
About This Test
Who Should Get This Test
To assess sperm DNA integrity by measuring the percentage of sperm with fragmented DNA. The result helps identify a hidden male factor in couples with unexplained infertility, recurrent pregnancy loss, failed IVF/ICSI attempts, or known risk factors such as varicocele, smoking, scrotal heat exposure, and chronic infection.
How to Prepare
- Follow the advised abstinence period before sample collection.
- Collect the sample directly into the sterile container.
- Avoid lubricants, condoms and saliva.
- Label the container with your name, date and time of collection.
- Transport the sample to the laboratory within the recommended time.
Doctor's Notes
Reviewed by Dr SHAILAJA RAGHUNATH MURDESHWAR — MBBS, MD (General Medicine) · Reg. No. 8052
"Sperm DNA fragmentation testing is valuable for couples facing unexplained infertility, recurrent pregnancy loss, or repeated IVF failure; it complements routine semen analysis and helps guide treatment planning."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Leaked or incorrectly labelled container
- Sample collected using lubricant, condom or saliva
- Sample received after 48 hours of storage
- Improper handling or temperature exposure
- Inadequate sample volume
Understanding Your Results
Sperm DNA integrity is acceptable; other fertility factors should be assessed.
Range: Low DFI
Improvement in lifestyle and treatment of contributing factors may be advised before ART.
Range: Moderate DFI
Sperm DNA damage is significant; further andrological and genetic evaluation is recommended.
Range: High DFI
If your DFI result is high, or if you and your partner have had unexplained infertility, recurrent miscarriages, or failed IVF/ICSI cycles, consult a reproductive medicine specialist or andrologist for a full fertility work-up and management plan.
Limitations
- ⚠A single DFI result is not a direct diagnosis of infertility and should be interpreted with full clinical history.
- ⚠The test does not identify the underlying cause of sperm DNA damage.
- ⚠Results cannot independently predict pregnancy or live-birth outcomes.
- ⚠Different laboratories may use different thresholds; serial comparisons should be done at the same laboratory.
Risks & Considerations
- ●No significant physical risks are expected
- ●Possible embarrassment or discomfort during semen collection
Interfering Factors
- ●Improper abstinence period before sample collection
- ●Use of lubricants, condoms or saliva during sample collection
- ●Smoking, alcohol and recreational drug use
- ●Scrotal heat exposure
- ●Varicocele or chronic genital tract infection
- ●Delayed sample processing or improper transport
Compare With Similar Tests
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Frequently Asked Questions
What is the cost of the Sperm DNA Fragmentation DFI Test?
What sample is required for the DFI test?
Is fasting required for this test?
What is measured in the test?
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How is the test performed?
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What does a high DFI result mean?
Can a normal routine semen analysis rule out sperm DNA damage?
What should I do after getting a high DFI report?
Reference Laboratory Services
We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.
Your Data Privacy
Your medical data is protected under Indian law.
✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.
✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.
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