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DNA Labs India

Vitamin B6 Pyridoxine Test

DNA Labs India | ISO 9001:2015 Certified

Vitamin B6 Pyridoxine Test

Short Name: Vitamin B6 Test

Also known as: Pyridoxine Blood Test, Vitamin B6 Blood Test, PLP Test, Pyridoxal Phosphate Test

Vitamin B6 Pyridoxine Test test available at DNA Labs India for ₹10,507. Uses LC-MS/MS on Plasma samples. Results in Samples received by the 7th of the month are processed and results are delivered after 2–3 weeks (14–21 days). Reports will be available through the patient portal, by email, and on WhatsApp, typically within this timeframe.. Free home collection in 300+ cities across India.

Quantitative🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Pasupathy Arumugam

Dr Pasupathy Arumugam

Consultant Pathologist · Reg: 21521

Last reviewed: September 7, 2026

Overview

The primary purpose of the Vitamin B6 Pyridoxine test is to determine whether an individual has adequate levels of Vitamin B6 in the blood. It is used to diagnose deficiency in patients presenting with suggestive symptoms, to evaluate nutritional status in high-risk populations (alcoholism, malabsorption, renal insufficiency), to monitor the effect of drugs that deplete Vitamin B6 (such as isoniazid and penicillamine), and to confirm suspected toxicity in patients taking excessive Vitamin B6 supplements. The test aids clinicians in creating a targeted supplementation or dietary adjustment plan.

Test Code
3734
CPT Code
84207
ICD Code
E53.1
Price
₹10,507
Sample Type
Plasma
Result Time
Samples received by the 7th of the month are processed and results are delivered after 2–3 weeks (14–21 days). Reports will be available through the patient portal, by email, and on WhatsApp, typically within this timeframe.
Fasting Required
Yes (8 hours)
Method
LC-MS/MS
Step 1

Sample Collection

Overnight fasting is mandatory. Do not eat or drink (except water) for at least 8 hours prior to blood collection. Inform the laboratory about any medications or supplements you are taking, especially Vitamin B6 supplements, which should be discontinued 24 hours before the test (only if considered safe by your physician). The sample must be collected early in the morning. Your blood sample will be drawn into a lavender-top EDTA tube, placed in an aluminium foil cover, and centrifuged to separate plasma within 3 hours. The separated plasma will be frozen and shipped to the reference laboratory.

Method: Venipuncture

Step 2

Laboratory Analysis

A trained phlebotomist will draw a small amount of blood (2 mL) from a vein in your arm. The procedure is quick and typically takes less than 5 minutes. Some individuals may experience a mild stinging sensation or a small bruise at the puncture site.

Step 3

Report Delivery

After blood collection, you can resume normal activities and eating immediately. A small bandage will be applied to the puncture site. It is advisable to keep it dry for a few hours. If you feel lightheaded, rest for a few minutes. You will receive a notification when your report is ready.

Timeline: Samples received by the 7th of the month are processed and results are delivered after 2–3 weeks (14–21 days). Reports will be available through the patient portal, by email, and on WhatsApp, typically within this timeframe.

Patient Instructions

1
Before the Test:Overnight fasting (8-12 hours) is mandatory. Do not consume Vitamin B6 supplements or multivitamins 24 hours before the test. Inform your physician and phlebotomist about any ongoing medications. The sample must be collected in the morning, and the tube must be protected from light immediately after collection. Complete the necessary Test Send Out Consent Form (Form 35).
2
During the Test:A blood sample (2 mL) will be drawn from a vein in your arm into a lavender-top EDTA tube. The phlebotomist will wrap the tube in aluminum foil and centrifuge it to separate plasma. The procedure is safe and carries minimal risk.
3
After the Test:You can leave after the blood draw. A bandage will be applied to the puncture site. No special precautions are required. The sample will be shipped frozen in a light-protected container to a reference laboratory. You will receive a report via email, WhatsApp, or the online portal once ready.

About This Test

Who Should Get This Test

The primary purpose of the Vitamin B6 Pyridoxine test is to determine whether an individual has adequate levels of Vitamin B6 in the blood. It is used to diagnose deficiency in patients presenting with suggestive symptoms, to evaluate nutritional status in high-risk populations (alcoholism, malabsorption, renal insufficiency), to monitor the effect of drugs that deplete Vitamin B6 (such as isoniazid and penicillamine), and to confirm suspected toxicity in patients taking excessive Vitamin B6 supplements. The test aids clinicians in creating a targeted supplementation or dietary adjustment plan.

How to Prepare

  • Sample must be collected in a Lavender Top (EDTA) tube
  • Wrap the tube in aluminium foil to protect it from light
  • Separate plasma within 3 hours of collection
  • Freeze the separated plasma immediately at -20°C or lower
  • Ship frozen - DO NOT THAW at any point
  • Duly filled Test Send Out Consent Form (Form 35) is mandatory

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"Vitamin B6 deficiency can present with nonspecific symptoms such as fatigue, depression, and neuropathy. A lab assessment is essential for accurate diagnosis and appropriate supplementation."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypePlasma
Sample Volume2 mL (1 mL min.)
ContainerLavender Top (EDTA) tube
Collection MethodVenipuncture

Sample Stability

Room Temperature (20-25°C)
Refrigerator (2-8°C)
Frozen (-20°C)
Sample Rejection Criteria:
  • Sample exposed to light
  • Sample received thawed
  • Hemolyzed or lipemic sample
  • Improper sample tube (not EDTA)
  • Incomplete/missing Test Send Out Consent Form
  • Sample collected >3 hours without plasma separation
  • Fasting not followed

Understanding Your Results

The Vitamin B6 Pyridoxine Test result is interpreted based on the plasma concentration of pyridoxal phosphate (PLP), the active coenzyme form of Vitamin B6. The reference range provided is 20-125 nmol/L. Values outside this range may indicate deficiency or excess.
📊

Deficiency (<20 nmol/L)

📊

Adequate (20-125 nmol/L)

📊

Elevated (>125 nmol/L)

⚠️ When to Consult a Doctor:

Consult your physician if you have symptoms such as persistent tingling or numbness in hands or feet, unexplained fatigue, confusion, seizures, anemia, or recurrent infections. Also, if you are taking medications like isoniazid or have a condition causing malabsorption, discuss whether a Vitamin B6 test is appropriate. A doctor will help interpret your result and recommend the right treatment plan.

Limitations

  • ⚠Plasma pyridoxine levels only reflect short-term dietary intake and may not represent long-term body stores
  • ⚠The test may not differentiate between various forms of Vitamin B6 (pyridoxal, pyridoxamine, their phosphates)
  • ⚠Results must be interpreted in conjunction with clinical history and other laboratory markers
  • ⚠Variations in reference ranges exist between laboratories; results should be compared with the specific laboratory's reference range
  • ⚠Falsely low results may occur if the sample is not protected from light or is frozen after prolonged storage

Risks & Considerations

  • ●Slight pain or bruising at the site of needle insertion
  • ●Dizziness or fainting during blood draw
  • ●Minimal risk of infection at the puncture site (very rare)
  • ●Hematoma (accumulation of blood under the skin)

Interfering Factors

  • ●Vitamin B6 supplements (including multivitamins) taken within 24 hours before the test may elevate levels
  • ●Certain medications such as isoniazid, penicillamine, hydralazine, and oral contraceptives may lower Vitamin B6 levels
  • ●Sample exposure to light for extended periods can reduce pyridoxine, leading to falsely low results
  • ●Hemolyzed or thawed samples may produce inaccurate results
  • ●Recent alcohol intake may decrease plasma pyridoxal phosphate
  • ●Pregnancy and lactation can alter Vitamin B6 levels

Compare With Similar Tests

TestVitamin B6 Pyridoxine TestVitamin B12 TestFolic Acid TestHomocysteine TestComplete Blood Count (CBC)
ComparisonVitamin B6 Pyridoxine Test

Frequently Asked Questions

What is the Vitamin B6 Pyridoxine Test?
The Vitamin B6 Pyridoxine Test measures the level of pyridoxine (Vitamin B6) in a blood plasma sample. It helps identify a deficiency or excess of Vitamin B6, which is essential for neurological, immune, and metabolic functions.
What is the cost of the Vitamin B6 Pyridoxine Test at DNA Labs India?
The test costs INR 10,506.6. This price includes free home sample collection and can be booked online. A discounted price of 10506.6 INR is applicable across all serviceable cities.
Is fasting required for the Vitamin B6 Pyridoxine Test?
Yes, overnight fasting (8-12 hours) is mandatory. Do not eat or drink anything except water for at least 8 hours before sample collection.
What is the recommended sample for the Vitamin B6 test?
A plasma sample is required. 2 mL (minimum 1 mL) of plasma from a lavender-top EDTA tube is collected. The tube must be wrapped in aluminium foil to protect it from light.
Why is the sample wrapped in aluminium foil?
Vitamin B6 is light-sensitive. Exposure to light degrades pyridoxine in the sample, which can lead to falsely low results. Protecting the tube in aluminium foil ensures an accurate measurement.
How soon will I get the report?
If the sample is received by the 7th of the month, reports are issued after 2–3 weeks. This is because the test is performed using LC-MS/MS at a reference laboratory.
What is the normal range of Vitamin B6 in plasma?
The reference range for plasma Vitamin B6 (pyridoxal phosphate) is typically 20-125 nmol/L. Values below 20 nmol/L indicate deficiency, and above 125 nmol/L may indicate excess.
What are the symptoms of Vitamin B6 deficiency?
Symptoms include depression, anemia, nausea, tingling in hands and feet, convulsions, confusion, difficulty walking, weakened immune system, and memory loss.
Can medications affect Vitamin B6 levels?
Yes, certain medications like isoniazid, penicillamine, hydralazine, and oral contraceptives can lower Vitamin B6 levels. Always inform your physician and the lab about any medicines you are taking.
Is home sample collection available?
Yes, DNA Labs India offers free home sample collection for online bookings in over 500 cities across India, including Mumbai, Delhi, Bangalore, Hyderabad, and many more.
Who should consider this test?
Individuals with symptoms like peripheral neuropathy, unexplained anemia, depression, recurrent infections, or those at risk for deficiency (alcoholism, malabsorption, drug-induced depletion) should consider this test.
How is the Vitamin B6 test performed?
The test is performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), a highly accurate and sensitive method for measuring pyridoxine levels in the blood.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.
For Hospitals & Clinics

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We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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