Alcohol Ethyl Glucuronide (EtG) & Ethyl Sulphate (EtS) Quantitative Assay Urine Test
Short Name: EtG & EtS Urine Test
Also known as: EtG EtS Urine Test, Alcohol Metabolites Urine Assay, Ethyl Glucuronide and Ethyl Sulphate Test, Alcohol Abstinence Monitoring Test, EtG Ethanol Metabolite Test
Alcohol Ethyl Glucuronide (EtG) & Ethyl Sulphate (EtS) Quantitative Assay Urine Test test available at DNA Labs India for ₹5,000. Uses LC-MS/MS (Liquid Chromatography–Tandem Mass Spectrometry) on Urine samples. Results in Sample must reach the laboratory by Friday 6 PM. Reports are available by the following Saturday.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
The primary purpose of the Alcohol EtG & EtS Quantitative Assay Urine Test is to objectively determine whether an individual has consumed alcohol within the preceding 3 to 4 days. Unlike self-reporting or traditional alcohol markers, EtG and EtS provide a biological, evidence-based confirmation of ethanol exposure. The quantitative nature of the report allows clinicians to assess the level of exposure and differentiate between incidental contact with alcohol-containing products and actual ingestion. This test is widely used for abstinence monitoring in substance abuse treatment programs, workplace alcohol screening, probation and parole compliance, custody and family court cases, fitness-for-duty evaluations, and pre-surgical or pre-transplant alcohol clearance assessments.
- Test Code
- 82
- CPT Code
- 80307
- ICD Code
- F10.10
- Price
- ₹5,000
- Sample Type
- Urine
- Result Time
- Sample must reach the laboratory by Friday 6 PM. Reports are available by the following Saturday.
- Fasting Required
- No
- Method
- LC-MS/MS (Liquid Chromatography–Tandem Mass Spectrometry)
Sample Collection
No special preparation such as fasting is required. Inform your healthcare provider about any medications, mouthwash use, or exposure to alcohol-containing products. Hydrate normally but avoid excessive fluid intake before sample collection.
Method: Clean-catch midstream urine collection
Laboratory Analysis
Collect a clean-catch midstream urine sample in the provided sterile screw-capped container. Ensure the container is free from contaminants and preservatives.
Report Delivery
The urine sample must be shipped refrigerated or frozen to maintain analyte stability. Do not leave the sample at room temperature for more than 6 hours.
Timeline: Sample must reach the laboratory by Friday 6 PM. Reports are available by the following Saturday.
Patient Instructions
About This Test
Who Should Get This Test
The primary purpose of the Alcohol EtG & EtS Quantitative Assay Urine Test is to objectively determine whether an individual has consumed alcohol within the preceding 3 to 4 days. Unlike self-reporting or traditional alcohol markers, EtG and EtS provide a biological, evidence-based confirmation of ethanol exposure. The quantitative nature of the report allows clinicians to assess the level of exposure and differentiate between incidental contact with alcohol-containing products and actual ingestion. This test is widely used for abstinence monitoring in substance abuse treatment programs, workplace alcohol screening, probation and parole compliance, custody and family court cases, fitness-for-duty evaluations, and pre-surgical or pre-transplant alcohol clearance assessments.
How to Prepare
- Wash hands thoroughly before collection
- Use the sterile screw-capped container provided by the lab
- Collect a midstream urine sample to minimise contamination
- Ensure a minimum of 2 mL (ideally 5 mL) of urine is collected
- Do not add any preservatives to the container
- Seal the container tightly and label with your name and date
- Ship the sample refrigerated or frozen; avoid prolonged room temperature storage
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
"The EtG and EtS urine test is one of the most reliable biomarkers available for detecting recent alcohol consumption, even when an individual denies drinking. These direct metabolites of ethanol are produced in the body only when alcohol is consumed, making them highly specific. A quantitative result helps clinicians distinguish between incidental exposure (such as from mouthwash or hand sanitiser) and genuine alcohol intake, which is invaluable in treatment monitoring, occupational health screening, and medicolegal evaluations. I recommend this test for patients in alcohol rehabilitation programs and for individuals subject to abstinence-based monitoring."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Sample received at room temperature beyond 6 hours of collection
- Sample collected with preservatives
- Insufficient sample volume (less than 2 mL)
- Unlabelled or improperly labelled container
- Visibly contaminated or adulterated sample
Understanding Your Results
No evidence of significant alcohol consumption within the detection window. Values in this range are considered negative for recent drinking.
Range: EtG < 500 ng/mL and EtS < 150 ng/mL
Low positive. May indicate minimal alcohol intake or incidental exposure to alcohol-containing products (e.g., mouthwash, hand sanitiser). Clinical correlation is recommended.
Range: EtG 500–999 ng/mL or EtS 150–249 ng/mL
Moderate positive. Suggests definite alcohol consumption within the detection window. Review for compliance if the individual is on an abstinence programme.
Range: EtG 1000–4999 ng/mL or EtS 250–999 ng/mL
High positive. Strongly indicative of significant alcohol consumption within the preceding 80 hours. Immediate clinical review and intervention may be warranted.
Range: EtG ≥ 5000 ng/mL or EtS ≥ 1000 ng/mL
If your EtG and/or EtS results are above the reference range, consult your treating physician, psychiatrist, or addiction medicine specialist immediately. A positive result may require counselling, adjustment of your treatment plan, or further clinical evaluation. Even if you believe the positive result is due to incidental exposure, it is important to discuss this with your healthcare provider for proper documentation and guidance.
Limitations
- ⚠This test detects alcohol consumption but does not indicate the quantity of alcohol consumed or the degree of impairment
- ⚠Cannot distinguish between different types of alcoholic beverages
- ⚠False positives may occur with incidental exposure to alcohol-containing products such as mouthwash or hand sanitiser
- ⚠EtG levels may be affected by urinary dilution; creatinine-normalised results may be requested for interpretation
- ⚠The test is not suitable for determining current blood alcohol concentration or acute intoxication
- ⚠Results should always be interpreted in the clinical context by a qualified healthcare professional
Risks & Considerations
- ●There are no physical risks associated with urine sample collection
- ●A false positive may occur due to incidental exposure to alcohol-containing products such as mouthwash, hand sanitisers, or fermented foods
- ●Emotional or professional consequences may arise from positive results in workplace or legal settings; discuss concerns with your healthcare provider beforehand
Interfering Factors
- ●Use of alcohol-containing mouthwash, hand sanitisers, or topical preparations may produce low-level EtG/EtS detectable in urine
- ●Fermented foods and certain medications (e.g., cough syrups containing ethanol) can contribute to low-level positives
- ●Prolonged specimen storage at room temperature may cause degradation of EtG and EtS, potentially leading to falsely lower results
- ●Adulteration or dilution of the urine specimen by the patient may affect accuracy
- ●Certain metabolic conditions in liver disease may affect ethanol conjugation rates
Compare With Similar Tests
| Test | Alcohol Ethyl Glucuronide (EtG) & Ethyl Sulphate (EtS) Quantitative Assay Urine Test | Blood Alcohol Concentration (BAC) | Breath Alcohol Test (Breathalyser) | Carbohydrate-Deficient Transferrin (CDT) | Phosphatidylethanol (PEth) | Gamma-Glutamyl Transferase (GGT) |
|---|---|---|---|---|---|---|
| Comparison | Alcohol Ethyl Glucuronide (EtG) & Ethyl Sulphate (EtS) Quantitative Assay Urine Test |
Frequently Asked Questions
What is the EtG & EtS Urine Test?
How long after drinking alcohol can EtG and EtS be detected in urine?
Can EtG and EtS tests produce false positives?
Do I need to fast before the EtG & EtS Urine Test?
What is the cost of the EtG & EtS Urine Test at DNA Labs India?
What sample is required for the EtG & EtS test?
How is the EtG & EtS test different from a breathalyser or blood alcohol test?
Is the EtG & EtS Urine Test suitable for legal and workplace purposes?
Can using mouthwash or hand sanitiser cause a positive EtG result?
How long does it take to get the EtG & EtS test results?
Is home sample collection available for this test?
What should I do if my EtG & EtS results are positive?
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