Skip to main content
DNA Labs India

Nicotine Screen Urine Test

DNA Labs India | ISO 9001:2015 Certified

Nicotine Screen Urine Test

Short Name: Nicotine Screen Urine

Also known as: Nicotine Test, Urine Nicotine Screen, Nicotine Detection Test

Nicotine Screen Urine Test test available at DNA Labs India for ₹761. Uses Lateral Flow Immunoassay on Urine samples. Results in Reports available same day if sample is received by 9 am.. Free home collection in 300+ cities across India.

Screening TestAll Ages🏠 Home Collection

🩺 Medically Reviewed By

Overview

To detect the presence and measure the amount of nicotine in urine, aiding in the diagnosis of nicotine addiction, monitoring smoking cessation, and evaluating exposure to tobacco products.

Test Code
1307
Price
₹761
Sample Type
Urine
Result Time
Reports available same day if sample is received by 9 am.
Fasting Required
No
Method
Lateral Flow Immunoassay
Step 1

Sample Collection

No special preparation required. Inform your healthcare provider about any medications or tobacco products used.

Method: Random urine collection

Step 2

Laboratory Analysis

Provide a mid-stream urine sample in the sterile container as instructed by the healthcare provider.

Step 3

Report Delivery

Ensure the container is sealed properly and stored as directed (refrigerated or frozen) until shipment.

Timeline: Reports available same day if sample is received by 9 am.

Patient Instructions

1
Before the Test:No special preparation required. Continue normal activities unless advised otherwise by your doctor.
2
During the Test:Simple urine collection process; non-invasive and typically takes a few minutes.
3
After the Test:Resume normal activities immediately. No downtime or risks associated with the test.

About This Test

Who Should Get This Test

To detect the presence and measure the amount of nicotine in urine, aiding in the diagnosis of nicotine addiction, monitoring smoking cessation, and evaluating exposure to tobacco products.

How to Prepare

  • Use a sterile screw capped container
  • Collect a random urine sample, minimum 10 mL
  • Ship the sample refrigerated or frozen to maintain stability
  • Label the container with patient details and date

Doctor's Notes

Reviewed by — MBBS, MD (Pathology) · Reg. No. 21521

"The Nicotine Screen Urine Test is essential for evaluating nicotine dependence, guiding cessation treatment, and preventing health complications from smoking."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypeUrine
Sample Volume15 mL (10 mL min.)
ContainerSterile screw capped container
Collection MethodRandom urine collection

Sample Stability

Room Temperature: 6 hours
Refrigerator (2-8°C): 48 hours
Frozen (-20°C): 4 weeks
Sample Rejection Criteria:
  • Insufficient sample volume (<10 mL)
  • Contaminated or unlabeled sample
  • Sample not stored at recommended temperature
  • Expired sample stability period

Understanding Your Results

Results indicate the presence or absence of nicotine in urine. A positive result suggests recent nicotine exposure, while a negative result indicates no detectable nicotine. Interpretation should be done by a healthcare professional considering clinical history.
📊

Positive

Nicotine detected, indicating recent use of tobacco products or nicotine-containing substances. Further evaluation for addiction severity is recommended.

📊

Negative

No nicotine detected, suggesting no recent exposure. However, this does not rule out past use or very low levels below detection limits.

⚠️ When to Consult a Doctor:

If the test is positive, or if symptoms of nicotine addiction (e.g., cravings, withdrawal symptoms) persist, consult a healthcare provider for personalized advice and treatment options.

Limitations

  • May not detect very low levels of nicotine
  • Potential for false positives due to cross-reactivity with other substances
  • Does not quantify exact nicotine levels; for precise measurement, quantitative tests are recommended
  • Results can be influenced by hydration status and urine concentration

Risks & Considerations

  • No significant risks as it is a non-invasive urine test
  • Possible minor discomfort during urination, but extremely rare

Interfering Factors

  • Recent use of nicotine products (e.g., cigarettes, patches, gums)
  • Certain medications that affect nicotine metabolism
  • Contamination of urine sample
  • Improper sample storage or handling

Compare With Similar Tests

TestNicotine Screen Urine TestCotinine Screen Urine TestBlood Nicotine TestSaliva Cotinine Test
ComparisonNicotine Screen Urine Test

Frequently Asked Questions

What is the Nicotine Screen Urine Test?
It is a laboratory test that detects nicotine in urine to assess addiction severity in smokers, aiding in diagnosis and treatment planning.
How much does the test cost in India?
The cost is INR 760.5, inclusive of home sample collection in many cities across India.
Is fasting required for this test?
No, fasting is not required. No special preparation is needed before sample collection.
How is the urine sample collected?
A random urine sample is collected in a sterile screw-capped container, with a minimum volume of 10 mL, and shipped refrigerated or frozen.
When will I receive the test results?
Results are typically available the same day if the sample is received by 9 am, and can be accessed online or via email/WhatsApp.
What does a positive result mean?
A positive result indicates the presence of nicotine, suggesting recent tobacco or nicotine product use, and may require further medical evaluation for addiction.
Is the test accurate and reliable?
Yes, the test uses lateral flow immunoassay, a validated method, and is conducted by experienced professionals at NABL-accredited labs for accuracy.
Can this test detect secondhand smoke exposure?
It may detect nicotine from secondhand smoke, but levels can vary; consult your doctor for interpretation based on exposure history.
How long does nicotine stay detectable in urine?
Nicotine can be detected in urine for 3-4 days after use, but this varies based on metabolism and usage patterns.
Are there any risks associated with the test?
No significant risks, as it is a simple, non-invasive urine test with no known side effects.
Is home sample collection available?
Yes, free home collection is offered for online bookings in numerous cities across India.
How should I prepare for the test?
No preparation is needed. Simply provide a urine sample as instructed and inform your doctor about any medications or tobacco use.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

Related Tests

For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

Your Data Privacy

Your medical data is protected under Indian law.

Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

Book Your Test

Enter your details and we'll connect you within 15 minutes.

🧬

Quick Connect

Enter your mobile number and we’ll connect you with the team.

+91

✅ Connecting you now...

🔒 Your number is used to respond to this request.