Cortisol Free 24 Hour Urine Test
Short Name: Free Cortisol 24Hr Urine
Also known as: 24-hour urine cortisol test, Free cortisol urine test, Urine free cortisol test, 24-hr UFC test
Cortisol Free 24 Hour Urine Test test available at DNA Labs India for ₹936. Uses Chemiluminescent Immunoassay on 10 mL (5 mL min.) aliquot of 24-hour urine samples. Results in 2-3 days. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Ramarao Paidisetty
Consultant Biochemist · Reg: 21504
Last reviewed: September 7, 2026
Overview
To diagnose adrenal gland disorders such as Cushing's syndrome and Addison's disease by measuring cortisol levels in urine.
- Test Code
- 353
- Price
- ₹936
- Sample Type
- 10 mL (5 mL min.) aliquot of 24-hour urine
- Result Time
- 2-3 days
- Fasting Required
- No
- Method
- Chemiluminescent Immunoassay
Sample Collection
No special preparation required. Inform your doctor about any medications you are taking, especially corticosteroids.
Method: 24-hour urine collection
Laboratory Analysis
Collect all urine over 24 hours in the provided container with boric acid. Record the total volume on the container.
Report Delivery
Ship the sample refrigerated or frozen to the laboratory.
Timeline: 2-3 days
Patient Instructions
About This Test
Who Should Get This Test
To diagnose adrenal gland disorders such as Cushing's syndrome and Addison's disease by measuring cortisol levels in urine.
How to Prepare
- Discard the first morning urine and start the 24-hour collection from that time.
- Collect all urine with 10 g of boric acid to maintain sample integrity.
- Record 24 hour volume on test request form and urine container.
- Keep the container refrigerated during collection period.
- Ship refrigerated or frozen to the laboratory.
Doctor's Notes
Reviewed by Dr Ramarao Paidisetty — MBBS, MD (Biochemistry) · Reg. No. 21504
"If you experience symptoms of cortisol imbalance, such as fatigue or weight gain, this test can help diagnose underlying conditions. Consult an endocrinologist for personalized advice."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Inadequate volume
- Sample not preserved with boric acid
- Specimen received at room temperature beyond 8 hours
Understanding Your Results
May indicate Cushing's syndrome, chronic stress, obesity, depression, or exogenous corticosteroid use.
Level: High Cortisol
May indicate Addison's disease, adrenal insufficiency, or hypopituitarism.
Level: Low Cortisol
Cortisol levels within the reference range suggest normal adrenal function.
Level: Normal Cortisol
If you experience persistent symptoms of cortisol imbalance or if your test results are abnormal, consult an endocrinologist for further evaluation and management.
Limitations
- ⚠A single 24-hour collection may not capture day-to-day cortisol variation
- ⚠Results should be correlated with clinical symptoms and other diagnostic tests
Risks & Considerations
- ●No significant risks as this is a non-invasive urine collection test.
Interfering Factors
- ●Recent physical or emotional stress
- ●Excessive fluid intake
- ●Certain medications such as corticosteroids
- ●Incomplete 24-hour urine collection
Compare With Similar Tests
| Test | Cortisol Free 24 Hour Urine Test | Cortisol Serum Test | Salivary Cortisol Test |
|---|---|---|---|
| Comparison | Cortisol Free 24 Hour Urine Test |
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