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DNA Labs India

Cultured AFB Antitubercular DST Amikacin Test

DNA Labs India | ISO 9001:2015 Certified

Cultured AFB Antitubercular DST Amikacin Test

Short Name: AFB DST Amikacin Test

Also known as: AFB Culture and Sensitivity Amikacin, TB Drug Sensitivity Test Amikacin

Cultured AFB Antitubercular DST Amikacin Test test available at DNA Labs India for ₹4,000. Uses Automated Fluorescent on Pure growth of Mycobacterium tuberculosis complex on appropriate media samples. Results in Report available within 21 days from sample receipt.. Free home collection in 300+ cities across India.

Culture and Sensitivity🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Kothari Ramesh Shantilal

Dr Kothari Ramesh Shantilal

Consultant Microbiologist · Reg: 27670

Last reviewed: September 7, 2026

Overview

The purpose of this test is to culture Mycobacterium tuberculosis from clinical samples and perform drug susceptibility testing for Amikacin to identify resistant strains, enabling targeted antibiotic therapy and improving treatment outcomes.

Test Code
422
Price
₹4,000
Sample Type
Pure growth of Mycobacterium tuberculosis complex on appropriate media
Result Time
Report available within 21 days from sample receipt.
Fasting Required
No
Method
Automated Fluorescent
Step 1

Sample Collection

No special preparation required. Avoid contaminating the sample with food or debris.

Method: Sputum collection or from existing culture

Step 2

Laboratory Analysis

Collect sputum in a sterile container as per instructions, or provide existing culture if available.

Step 3

Report Delivery

Store sample at refrigerator temperature and transport to lab promptly for analysis.

Timeline: Report available within 21 days from sample receipt.

Patient Instructions

1
Before the Test:No special preparation required. Inform your doctor of any medications or health conditions.
2
During the Test:Sputum sample collection or submission of existing culture to the lab.
3
After the Test:Wait for the detailed report, typically available in 21 days, and discuss results with your doctor.

About This Test

Who Should Get This Test

The purpose of this test is to culture Mycobacterium tuberculosis from clinical samples and perform drug susceptibility testing for Amikacin to identify resistant strains, enabling targeted antibiotic therapy and improving treatment outcomes.

How to Prepare

  • Use the provided sterile sputum collection kit
  • Collect morning sputum for best yield
  • Ensure container is sealed and labeled properly

Doctor's Notes

Reviewed by — MBBS, MD (Microbiology) · Reg. No. 27670

"Early and accurate detection of drug-resistant TB strains through DST is critical for effective treatment and preventing spread."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypePure growth of Mycobacterium tuberculosis complex on appropriate media
ContainerSterile screw capped container in a sealed plastic bag
Collection MethodSputum collection or from existing culture

Sample Stability

Room Temperature: 48 hours
Refrigerator: 4 weeks
Frozen: Not applicable
Sample Rejection Criteria:
  • Improperly labeled or contaminated samples
  • Samples stored beyond stability limits
  • Insufficient sample volume

Understanding Your Results

Results indicate the presence of Mycobacterium tuberculosis and its susceptibility to Amikacin. A positive culture with resistance suggests treatment adjustments may be needed.
📊

Culture Positive, Amikacin Sensitive

TB confirmed and likely responsive to Amikacin-based therapy.

📊

Culture Positive, Amikacin Resistant

TB confirmed with resistance to Amikacin; alternative antibiotics required.

📊

Culture Negative

No growth of Mycobacterium tuberculosis; consider other causes or repeat testing.

⚠️ When to Consult a Doctor:

Consult a doctor immediately if you have TB symptoms, test positive, or if symptoms persist after treatment.

Limitations

  • ⚠Tests only for Amikacin sensitivity, not comprehensive DST for all drugs
  • ⚠Requires pure culture growth, which may take weeks
  • ⚠False negatives possible if bacterial load is low

Risks & Considerations

  • ●Minimal risk from sputum collection, such as coughing discomfort
  • ●No significant risks associated with the test itself

Interfering Factors

  • ●Sample contamination
  • ●Improper sample storage or transport
  • ●Concurrent antibiotic use affecting culture growth

Frequently Asked Questions

What is the Cultured AFB Antitubercular DST Amikacin Test?
It is a laboratory test that cultures Mycobacterium tuberculosis from sputum samples and determines its sensitivity to the antibiotic Amikacin, aiding in TB diagnosis and treatment planning.
Why is this test important for tuberculosis?
This test helps identify drug-resistant TB strains, ensuring patients receive effective antibiotics and reducing the risk of treatment failure and spread.
How is the test performed?
A sputum sample is collected and cultured in the lab to grow the bacteria. Then, drug susceptibility testing is done to check if Amikacin can inhibit growth.
What is the cost of this test at DNA Labs India?
The test costs INR 4000, which includes sample collection kit, lab analysis, and a detailed report.
Is home sample collection available?
Yes, DNA Labs India offers free home collection across many cities in India for this test.
How long does it take to get the results?
Results are typically available within 21 days from sample submission due to the culture process.
What should I do if my test results are positive?
Consult your doctor immediately to discuss treatment options and prevent spreading TB to others.
Are there any special preparations for the test?
No special preparation is required, but ensure the sputum sample is collected properly to avoid contamination.
Can this test detect all types of TB?
It detects Mycobacterium tuberculosis complex strains and their sensitivity to Amikacin, but not all TB strains or other drugs.
What is the sample stability for this test?
The sample is stable at room temperature for 48 hours and refrigerated for up to 4 weeks.
Is this test covered by insurance?
Coverage depends on your insurance provider. Check with your insurer or PMJAY, CGHS, or other schemes for details.
How accurate is the Cultured AFB Antitubercular DST Amikacin Test?
It is a gold-standard method for detecting TB and drug sensitivity, with high accuracy when samples are handled properly.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

LIMS Integration

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Your medical data is protected under Indian law.

✓ Stored in India: All patient records are stored on servers located in India. No data is transferred outside the country.

✓ DPDP Act Compliant: Under the Digital Personal Data Protection Act 2023, you can request deletion of your records at any time by contacting support.

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