Cultured AFB Antitubercular DST Isoniazid Test
Short Name: Cultured AFB DST Isoniazid
Also known as: AFB Isoniazid DST, Isoniazid Drug Susceptibility Test, Cultured DST Isoniazid, MTB Isoniazid Susceptibility
Cultured AFB Antitubercular DST Isoniazid Test test available at DNA Labs India for ₹4,000. Uses Automated Fluorescent on Pure growth of Mycobacterium tuberculosis complex on appropriate media samples. Results in Report is usually available after 21 days from sample receipt.. Free home collection in 300+ cities across India.
🩺 Medically Reviewed By
Dr Kothari Ramesh Shantilal
Consultant Microbiologist · Reg: 27670
Last reviewed: September 7, 2026
Overview
The Cultured AFB Antitubercular DST Isoniazid Test is used to detect Mycobacterium tuberculosis and determine susceptibility to isoniazid. It helps guide treatment decisions in active TB and suspected drug-resistant TB.
- Test Code
- 3460
- Price
- ₹4,000
- Sample Type
- Pure growth of Mycobacterium tuberculosis complex on appropriate media
- Result Time
- Report is usually available after 21 days from sample receipt.
- Fasting Required
- No
- Method
- Automated Fluorescent
Sample Collection
No special preparation is required. The test requires a pure growth of Mycobacterium tuberculosis complex; primary sputum or other specimen may be collected earlier.
Method: Submit pure growth of Mycobacterium tuberculosis complex in sterile screw capped container
Laboratory Analysis
The sample should be handled aseptically and transported in a sterile screw-capped container within a sealed plastic bag.
Report Delivery
Keep the sample refrigerated during transport. The laboratory will process it promptly.
Timeline: Report is usually available after 21 days from sample receipt.
Patient Instructions
About This Test
Who Should Get This Test
The Cultured AFB Antitubercular DST Isoniazid Test is used to detect Mycobacterium tuberculosis and determine susceptibility to isoniazid. It helps guide treatment decisions in active TB and suspected drug-resistant TB.
How to Prepare
- Submit pure growth of Mycobacterium tuberculosis complex on appropriate medium
- Use sterile screw capped container inside sealed plastic bag
- Ship refrigerated
- If used with AFB culture, the pure growth obtained in laboratory may be processed
- Label sample with patient details and collection date
Doctor's Notes
Reviewed by Dr Kothari Ramesh Shantilal — MBBS, MD (Microbiology) · Reg. No. 27670
"Isoniazid is a backbone of first-line TB therapy. Phenotypic DST on a pure culture helps identify resistance and tailor treatment, especially in smear-positive or culture-positive cases."
Last medically reviewed: September 7, 2026
Test Parameters & Specifications
Sample Stability
- Contaminated or mixed bacterial growth
- Leaking or unsealed container
- Insufficient growth for susceptibility testing
- Unsuitable transport medium
- Mismatched patient details
Understanding Your Results
Susceptible
Isoniazid can be considered effective for the tested Mycobacterium tuberculosis isolate.
Resistant
Isoniazid is not likely to be effective; the clinician should review alternative treatment.
Intermediate/equivocal
Repeat testing or additional molecular resistance testing may be needed.
Consult an infectious disease specialist or pulmonologist if your result shows isoniazid resistance, if TB symptoms persist, or if you have been exposed to drug-resistant TB.
Limitations
- ⚠Requires pure growth; cannot be performed directly on all sputum samples
- ⚠Culture-based DST has a longer turnaround time
- ⚠Results should be interpreted with clinical context
- ⚠Does not detect all possible resistance mechanisms
- ⚠Does not replace clinical evaluation or public health notification
Risks & Considerations
- ●No direct risk to patient when performed on laboratory pure culture
- ●Primary sample collection may have minimal discomfort
Interfering Factors
- ●Improper sample transport or refrigeration
- ●Mixed or contaminated culture
- ●Non-viable organisms
- ●Prior antibiotic exposure
- ●Delayed processing beyond stability limits
Compare With Similar Tests
| Test | Cultured AFB Antitubercular DST Isoniazid Test | ||||
|---|---|---|---|---|---|
| Comparison | Cultured AFB Antitubercular DST Isoniazid Test |
Frequently Asked Questions
What is the Cultured AFB Antitubercular DST Isoniazid Test?
Why is isoniazid drug susceptibility testing done?
What sample type is required?
Is fasting required for this test?
What is the cost at DNA Labs India?
How long does it take to get reports?
What method is used for testing?
Can home sample collection be arranged?
What does isoniazid susceptible mean?
What does isoniazid resistant mean?
In which cities is the test available?
How should the culture sample be stored and transported?
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₹4,000Reference Laboratory Services
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