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Cultured AFB Antitubercular DST Rapid 10 Second Line Drugs Panel Test

DNA Labs India | ISO 9001:2015 Certified

Cultured AFB Antitubercular DST Rapid 10 Second Line Drugs Panel Test

Short Name: AFB DST Second Line Panel

Also known as: AFB DST Second Line Drugs Panel, Drug Susceptibility Test - Second Line Antitubercular Drugs, Cultured AFB DST SLD, Mycobacterium tuberculosis Second Line Drug Susceptibility Panel

Cultured AFB Antitubercular DST Rapid 10 Second Line Drugs Panel Test test available at DNA Labs India for ₹15,000. Uses Automated Fluorescent on Pure growth of Mycobacterium tuberculosis complex on appropriate media samples. Results in Sample accepted daily by 3:30 pm; report in 21 days.. Free home collection in 300+ cities across India.

Physician🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr Kothari Ramesh Shantilal

Dr Kothari Ramesh Shantilal

Consultant Microbiologist · Reg: 27670

Last reviewed: September 7, 2026

Overview

This test determines the susceptibility of cultured Mycobacterium tuberculosis complex to key second-line anti-tubercular drugs: Amikacin, Kanamycin, Capreomycin, Clofazimine, Ethionamide, Ofloxacin, Levofloxacin, Moxifloxacin, PAS, and Cycloserine.

Test Code
3465
Price
₹15,000
Sample Type
Pure growth of Mycobacterium tuberculosis complex on appropriate media
Result Time
Sample accepted daily by 3:30 pm; report in 21 days.
Fasting Required
No
Method
Automated Fluorescent
Step 1

Sample Collection

No special preparation required. For laboratory submission, ensure a pure growth of Mycobacterium tuberculosis complex is available on appropriate media.

Method: Submit pure growth of Mycobacterium tuberculosis complex on appropriate media under aseptic conditions; ship refrigerated

Step 2

Laboratory Analysis

Transfer the pure growth aseptically into a sterile screw-capped container and seal it in a plastic bag.

Step 3

Report Delivery

Ship refrigerated to the laboratory. If using an isolate from an already submitted specimen, no additional direct patient collection may be needed.

Timeline: Sample accepted daily by 3:30 pm; report in 21 days.

Patient Instructions

1
Before the Test:No special preparation is required. The test is performed on a cultured pure growth, not directly on a patient sample in most cases.
2
During the Test:A pure growth of Mycobacterium tuberculosis complex is processed using an automated fluorescent method for drug susceptibility testing.
3
After the Test:Report is delivered in about 21 days through the online portal, email, or WhatsApp.

About This Test

Who Should Get This Test

This test determines the susceptibility of cultured Mycobacterium tuberculosis complex to key second-line anti-tubercular drugs: Amikacin, Kanamycin, Capreomycin, Clofazimine, Ethionamide, Ofloxacin, Levofloxacin, Moxifloxacin, PAS, and Cycloserine.

How to Prepare

  • Submit pure growth of Mycobacterium tuberculosis complex on appropriate media in a sterile screw capped container.
  • Seal the container in a plastic bag.
  • Ship refrigerated to the laboratory.
  • When used with other AFB culture or Mycobacteria identification tests, the pure growth obtained in the laboratory from already submitted specimens will be processed for antitubercular sensitivity.

Doctor's Notes

Reviewed by — MBBS, MD (Microbiology) · Reg. No. 27670

"This culture-based second-line DST is essential for guiding treatment in drug-resistant tuberculosis. Interpret the report alongside clinical history, sputum bacteriology, and local drug-resistance patterns."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample TypePure growth of Mycobacterium tuberculosis complex on appropriate media
Sample VolumeNot applicable
ContainerSterile screw capped container in sealed plastic bag
Collection MethodSubmit pure growth of Mycobacterium tuberculosis complex on appropriate media under aseptic conditions; ship refrigerated

Sample Stability

Room Temperature48 hrs
Refrigerator4 weeks
FrozenNot applicable
Sample Rejection Criteria:
  • Mixed or contaminated cultures
  • Improper transport temperature
  • Leaking or unlabeled specimen container
  • Insufficient growth or non-viable organism

Understanding Your Results

Results are reported as susceptible or resistant for each second-line drug tested. These interpretations guide the selection of an effective anti-tubercular regimen.
📊

Susceptible

The drug is considered likely to be effective at standard dosing against the isolated strain.

📊

Resistant

The drug is considered unlikely to be effective; the clinician should consider an alternative agent or regimen.

⚠️ When to Consult a Doctor:

Consult an infectious disease specialist or pulmonologist if you have persistent cough, fever, night sweats, unintentional weight loss, coughing blood, or if this test shows drug-resistant tuberculosis.

Limitations

  • ⚠Requires a pure culture isolate of Mycobacterium tuberculosis complex
  • ⚠May not reliably detect heteroresistance in all cases
  • ⚠Results should be correlated with clinical response and local resistance patterns
  • ⚠Some second-line drugs may have limited reproducibility in culture-based DST

Risks & Considerations

  • ●No direct patient risk because the test is performed on a cultured isolate.
  • ●Laboratory personnel handle mycobacterial cultures under biosafety precautions.

Interfering Factors

  • ●Mixed or contaminated culture
  • ●Non-viable or insufficient mycobacterial growth
  • ●Improper specimen transport temperature
  • ●Technical factors related to automated fluorescent testing

Compare With Similar Tests

TestCultured AFB Antitubercular DST Rapid 10 Second Line Drugs Panel Test
ComparisonCultured AFB Antitubercular DST Rapid 10 Second Line Drugs Panel Test

Frequently Asked Questions

What is Cultured AFB Antitubercular DST Rapid 10 Second Line Drugs Panel Test?
It is a laboratory test that uses a pure culture of Mycobacterium tuberculosis complex to check susceptibility to ten second-line anti-TB drugs.
Why is this test performed?
It is performed to guide second-line anti-TB treatment, especially in multidrug-resistant TB or when first-line treatment has failed.
What sample is required for the test?
A pure growth of Mycobacterium tuberculosis complex on appropriate media in a sterile screw-capped container in a sealed plastic bag, shipped refrigerated.
Is fasting required before the test?
No special preparation or fasting is required.
Which drugs are tested in this panel?
The panel tests Amikacin, Kanamycin, Capreomycin, Clofazimine, Ethionamide, Ofloxacin, Levofloxacin, Moxifloxacin, PAS, and Cycloserine.
How is the test performed?
The test is performed using an automated fluorescent method on a cultured isolate.
What is the turnaround time for this test?
The report is typically available in approximately 21 days after sample receipt.
What does a 'Susceptible' result mean?
Susceptible means the drug is likely effective against the isolated TB strain at standard dosing.
What does a 'Resistant' result mean?
Resistant means the drug is unlikely to be effective, and an alternative treatment agent or regimen may be needed.
What is the price of this test in India?
The price is Rs 15000 at DNA Labs India, but it may vary by laboratory.
When should I consult a doctor?
Consult a doctor if you have persistent cough, fever, night sweats, unintentional weight loss, coughing blood, or if the report shows drug-resistant TB.
Is home collection available for this test?
DNA Labs India offers home sample collection for online bookings, but this test requires a culture isolate; the laboratory coordinates sample submission.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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