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DNA Labs India

Hemoglobin HPLC/Electrophoresis Test

DNA Labs India | ISO 9001:2015 Certified

Hemoglobin HPLC/Electrophoresis Test

Short Name: Hb HPLC/Electrophoresis

Also known as: Hemoglobin Electrophoresis, HPLC Hemoglobin Test

Hemoglobin HPLC/Electrophoresis Test test available at DNA Labs India for ₹1,112. Uses High Performance Liquid Chromatography, Electrophoresis on 4 mL (2 mL min.) whole blood from 1 Lavender Top (EDTA) tube. samples. Results in Same day, if sample received by 10 am.. Free home collection in 300+ cities across India.

PhysicianAll Ages🏠 Home Collection

✍️ Written By

🩺 Medically Reviewed By

Dr SHAILAJA RAGHUNATH MURDESHWAR

Dr SHAILAJA RAGHUNATH MURDESHWAR

Consultant Physician · Reg: 8052

Last reviewed: September 7, 2026

Overview

This assay is useful in the diagnosis of Beta Thalassemia. It quantitates the percent of fetal hemoglobin and assists in the diagnosis of disorders with elevated levels of HbF.

Test Code
698
Price
₹1,112
Sample Type
4 mL (2 mL min.) whole blood from 1 Lavender Top (EDTA) tube.
Result Time
Same day, if sample received by 10 am.
Fasting Required
No
Method
High Performance Liquid Chromatography, Electrophoresis
Step 1

Sample Collection

Record date of birth, sex, race, and religion on the test request form. No fasting required.

Method: Venipuncture

Step 2

Laboratory Analysis

A blood sample will be drawn from a vein in your arm using a needle and collected in an EDTA tube.

Step 3

Report Delivery

Apply pressure to the puncture site to stop bleeding. Resume normal activities unless advised otherwise.

Timeline: Same day, if sample received by 10 am.

Patient Instructions

1
Before the Test:No special preparation needed. Provide accurate demographic details on the test form.
2
During the Test:A healthcare professional will draw blood from your arm, which takes a few minutes.
3
After the Test:You may experience minor bruising or pain at the puncture site. Results will be available via online portal, email, or WhatsApp.

About This Test

Who Should Get This Test

This assay is useful in the diagnosis of Beta Thalassemia. It quantitates the percent of fetal hemoglobin and assists in the diagnosis of disorders with elevated levels of HbF.

How to Prepare

  • Record date of birth, sex, race, and religion on the test request form.
  • Collect 4 mL (2 mL min.) whole blood in a Lavender Top (EDTA) tube.
  • Ship refrigerated; do not freeze.
  • Ensure sample stability: Room Temperature: 6 hrs, Refrigerator: 48 hrs, Frozen: NA

Doctor's Notes

Reviewed by — MBBS, MD (General Medicine) · Reg. No. 8052

"This test is essential for diagnosing hemoglobinopathies like thalassemia and sickle cell disease, guiding treatment and family planning."

Last medically reviewed: September 7, 2026

Test Parameters & Specifications

Sample Type4 mL (2 mL min.) whole blood from 1 Lavender Top (EDTA) tube.
Sample Volume4 mL (2 mL min.)
ContainerLavender Top (EDTA) tube
Collection MethodVenipuncture

Sample Stability

Room Temperature: 6 hours
Refrigerator: 48 hours
Frozen: Not Acceptable
Sample Rejection Criteria:
  • Insufficient sample volume
  • Hemolyzed or clotted sample
  • Improper container or labeling
  • Sample not shipped refrigerated

Understanding Your Results

Results are interpreted based on the percentage of different hemoglobin types. Abnormal levels may indicate specific hemoglobin disorders.
Elevated HbA2 ( >3.5%) suggests beta-thalassemia trait.
High HbF (>1% in adults) may indicate thalassemia major, hereditary persistence of fetal hemoglobin, or other conditions.
Presence of HbS indicates sickle cell disease or trait.
Absence of HbA with other variants may indicate homozygous disorders.
⚠️ When to Consult a Doctor:

Consult your doctor if results are abnormal, if you have symptoms like chronic anemia, jaundice, or if you have a family history of hemoglobin disorders.

Limitations

  • ⚠May not detect all rare hemoglobin variants
  • ⚠Requires specialized equipment and trained personnel
  • ⚠Results may be influenced by sample quality

Risks & Considerations

  • ●Minor pain or bruising at the blood draw site
  • ●Rare risk of infection or excessive bleeding

Interfering Factors

  • ●Recent blood transfusion
  • ●Certain medications
  • ●Sample hemolysis
  • ●Improper sample storage

Frequently Asked Questions

What is the Hemoglobin HPLC/Electrophoresis test?
It is a blood test that separates and quantifies different types of hemoglobin to diagnose disorders like thalassemia and sickle cell disease.
Why is this test recommended?
It is recommended for diagnosing hemoglobinopathies, monitoring hemoglobin levels, and investigating symptoms of anemia or jaundice.
What is the cost of the test in India?
At DNA Labs India, the test costs INR 1111.5, with free home sample collection available across many cities.
How is the test performed?
A blood sample is drawn from a vein and analyzed using HPLC or electrophoresis to identify hemoglobin variants.
Is fasting required for the test?
No, fasting is not required. You can eat and drink normally before the test.
How long does it take to get results?
Results are typically available the same day if the sample is received by 10 am.
What do abnormal results indicate?
Abnormal results may indicate conditions such as beta-thalassemia, sickle cell disease, or other hemoglobin disorders.
Is the test safe?
Yes, it is a standard blood test with minimal risks, such as minor bruising at the puncture site.
Can the test be done at home?
Yes, DNA Labs India offers free home sample collection for this test across India.
How accurate is the test?
The test is highly accurate and considered a gold standard for diagnosing hemoglobin disorders when performed in accredited labs.
What is the difference between HPLC and electrophoresis?
Both methods separate hemoglobin variants; HPLC is more automated and quantifies components, while electrophoresis visualizes patterns.
Who should get this test?
Individuals with symptoms of anemia, jaundice, or a family history of hemoglobin disorders, as well as for carrier screening or newborn screening.
Worried about the process? Our certified phlebotomists collect thousands of samples every month across India. The process takes under 5 minutes and is virtually painless. Questions? Message us on WhatsApp — we're here 7 days a week.

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For Hospitals & Clinics

Reference Laboratory Services

We serve as a reference laboratory for hospitals and clinics across India. Send samples from your facility with same-day pickup, priority processing, and results delivered through our online portal. Competitive institutional pricing available.

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